Study of Laser Light Scattering Methods in Rapid Viability Assessment of Microorganisms under Antibiotics Exposure for Adaptation in Lab-on-A-Chip Format.

The antibiotic resistance (ABR) problem is becoming increasingly disturbing and it is important to implement express methods of ABR testing to allow operative antibiotic therapy decisions. The application of laser light scattering (LLS) in microbiological analysis for express ABR testing of microorganisms has been considered. The ways of miniaturization of laser light scattering for creating the bases of their integration into microbiological laboratory-on-a-chip (MLOC) for clinical express diagnostics have been analysed. The advantage of miniaturization in the context of clinical express analysis realization problems are investigated. A system of parallel measuring cells and illumination, enabling simultaneous testing of a group of antibiotics, was tested by splitting a laser beam with a two-dimensional collimator prepared of nanoporous anodic aluminum oxide. It has been demonstrated that the application of LLS methods, providing high concentration and mass sensitivity as well as a miniaturization potential, is an effective approach in the development of new generation diagnostic instruments. The studies have demonstrated the ability of methods to register effects of antibiotics on microbiological samples within 10 min. The following microorganisms were used in the study: Escherichia coli M-17, Lactobacillus plantarum, Bifidobacterium bifidum, Stenotrophomonas maltophilia.

Case Report: Oncocytic Schneiderian Papilloma Originating From the Sphenoid Sinus.

A rare case of oncocytic Schneiderian papilloma originating from the sphenoid sinus characterised, for 3 years, by non-specific symptoms of severe headache, a block of nasal breathing, and deprecating sense of smell was presented by an elderly female patient. Sphenoid sinus functional endoscopic sinus surgery (FESS), with a one-block tumour excision, through an endonasal approach, with a histological study of removed tumour masses, were performed on the patient. Long observation in the post-operative period was necessary, considering the risk of recurrence and malignancy of oncocytic Schneiderian papilloma (OSP). Although the oncocytic papilloma of the sphenoid sinus is rare, non-specific symptoms make this pathology easily misdiagnosed. Thus, any isolated unilateral process in the paranasal sinuses with long-existing symptoms must be given careful attention due to the chance of this process being an inverted papilloma with malignization. CT scan indicating a unilateral opacification of paranasal sinuses with local calcifications is a typical manifestation, and endoscopic sphenoidotomy can be recommended as a treatment of choice.