ABSTRACT
We have developed an automated purification method for dye-terminator-based DNA sequencing products using a magnetic bead approach. This 384-well protocol generates sequence fragments that are essentially free of template DNA, salt, and excess dye-terminator products. In comparison with traditional ethanol precipitation protocols, this method uses no centrifugation, is rapid, completely automated, and increases the phred-20 read length by an average of 40 bases. To date, we have processed over 4 million samples with 94% averaging 641 phred-20 bases on the MegaBACE 1000 and 4000 and the ABI PRISM 3700 capillary instruments.
Subject(s)
DNA/isolation & purification , Electrophoresis, Capillary/methods , Ethanol/metabolism , Glycols/metabolism , Magnetics , Microspheres , Water/metabolismABSTRACT
The antibody response against Toxoplasma gondii was evaluated by ELISA and indirect haemagglutination test (IHA) in 100 antenatal cases with bad obstetric history. 30 per cent of the cases were found to be positive by the IgM ELISA test and 22 per cent were positive by the IHA test. ELISA was found to be a more specific, reliable and easy test for screening of antenatal cases.
Subject(s)
Enzyme-Linked Immunosorbent Assay , Hemagglutination Tests/methods , Pregnancy Complications, Infectious/diagnosis , Toxoplasmosis/diagnosis , Acute Disease , Female , Humans , PregnancySubject(s)
Blood Pressure/drug effects , Dioxanes/pharmacology , Dioxins/pharmacology , Anesthesia , Animals , Antihypertensive Agents , Dose-Response Relationship, Drug , Male , Norepinephrine/antagonists & inhibitors , Phentolamine/pharmacology , Phenylephrine/pharmacology , Rats , Sympathetic Nervous System/drug effectsABSTRACT
We have compared the influence of a series of benzodioxane alpha-adrenergic antagonists on 3H-WB-4101 and 3H-clonidine binding to alpha-receptor sites in the brain and peripheral tissues with their pharmacological properties. The drug specificity of 3H-WB-4101 binding is quite similar in central and peripheral tissues. Pharmacological potencies of benzodioxanes at postsynaptic alpha-receptors in the rat vas deferens correlate with potencies at 3H-WB-4101 but not at 3H-clonidine binding sites. These findings suggest pharmacological effects of these drugs are mediated by "alpha-1 postsynaptic receptors" labeled by 3H-WB-4101. For several benzodioxanes absolute pharmacological potencies at postsynaptic alpha-receptors of the rat vas deferens are substantially less than their potencies at 3H-WB-4101 sites. The potencies of benzodioxane analogues at 3H-clinidine binding sites are similar to their pharmacological potencies at presynaptic autoreceptors in the rat vas deferens.
Subject(s)
Piperidines/metabolism , Piperoxan/metabolism , Receptors, Adrenergic, alpha/metabolism , Receptors, Adrenergic/metabolism , Animals , Cerebral Cortex/metabolism , Clonidine/pharmacology , Hydroxydopamines/pharmacology , Male , Muscle Contraction/drug effects , Norepinephrine/metabolism , Piperoxan/analogs & derivatives , Piperoxan/pharmacology , Rats , Synaptosomes/metabolismSubject(s)
Adrenergic alpha-Antagonists/pharmacology , Dioxanes/pharmacology , Dioxins/pharmacology , Receptors, Serotonin/drug effects , Animals , Biogenic Amines/pharmacology , In Vitro Techniques , Male , Moxisylyte/pharmacology , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Phentolamine/pharmacology , Phenyl Ethers/pharmacology , Rats , Stomach/drug effects , Vas Deferens/drug effects , Yohimbine/pharmacologySubject(s)
Adrenergic alpha-Antagonists/pharmacology , Dioxanes/pharmacology , Dioxins/pharmacology , Receptors, Adrenergic, alpha/drug effects , Receptors, Adrenergic/drug effects , Animals , Drug Interactions , In Vitro Techniques , Male , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Phenyl Ethers/pharmacology , Rats , Structure-Activity Relationship , Vas Deferens/drug effectsSubject(s)
Adrenergic alpha-Antagonists/metabolism , Dioxanes/pharmacology , Dioxins/pharmacology , Receptors, Adrenergic, alpha/metabolism , Receptors, Adrenergic/metabolism , Adrenergic alpha-Antagonists/pharmacology , Animals , In Vitro Techniques , Male , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Phenyl Ethers/pharmacology , Rats , Structure-Activity RelationshipSubject(s)
Adrenergic alpha-Antagonists , Piperidines/pharmacology , Piperoxan/pharmacology , Animals , Clonidine/pharmacology , In Vitro Techniques , Male , Muscle Contraction/drug effects , Phentolamine/pharmacology , Piperoxan/analogs & derivatives , Rats , Synapses/drug effects , Yohimbine/pharmacologyABSTRACT
The effects of a number of drugs that deplete catecholamines or block their receptors were investigated on the levels of free amino acids and glutamate decarboxylase activity in rat striatum and substantia nigra, in an attempt to clarify the possible links between catecholamine and amino acid transmitter function. While none of the drugs tested were significantly active in the striatum, profound changes occurred in the substantia nigra. The data are discussed in relation to the possible mechanisms involved.
