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Vopr Virusol ; 60(6): 41-5, 2015.
Article in Russian | MEDLINE | ID: mdl-27024917

ABSTRACT

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno- chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.


Subject(s)
Antibodies, Viral/blood , Antigens, Viral/blood , Biological Assay , Influenza in Birds/diagnosis , Newcastle Disease/diagnosis , Poultry Diseases/diagnosis , Animals , Chickens , Colorimetry , Enzyme-Linked Immunosorbent Assay , Hemagglutination Inhibition Tests , Influenza A virus/immunology , Influenza in Birds/blood , Influenza in Birds/immunology , Influenza in Birds/virology , Luminescence , Newcastle Disease/blood , Newcastle Disease/immunology , Newcastle Disease/virology , Newcastle disease virus/immunology , Poultry Diseases/blood , Poultry Diseases/immunology , Poultry Diseases/virology , Sensitivity and Specificity
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