ABSTRACT
Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway.
Subject(s)
Antigens, Ly/physiology , Cell Proliferation , Interleukin-6/physiology , Membrane Proteins/physiology , STAT3 Transcription Factor/physiology , Signal Transduction/physiology , Submandibular Gland/physiopathology , Animals , Inflammation/etiology , Inflammation/physiopathology , Ligation/adverse effects , Male , Mice , Mice, Inbred C57BL , Regeneration/physiology , Submandibular Gland/cytologyABSTRACT
In the colony of Sprague-Dawley (SD) strain, we found that there were rats expressing a mutant AQP5, which has a point mutation at nt 308 (G308A), leading to a replacement of (103)Gly with (103)Asp in the 3rd transmembrane domain. The mutant molecule scarcely expressed in the acinar cells, probably because of ineffective trafficking. The mutant molecule, however, showed normal water permeability when assessed by the oocyte system.
