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1.
Acta Neurochir Suppl ; 118: 39-43, 2013.
Article in English | MEDLINE | ID: mdl-23564101

ABSTRACT

BACKGROUND: Heat-shock protein 90 (Hsp90) inhibitor geldanamycin was found to be neuroprotective in various experimental models of brain disease. The effect was attributed to the induction of heat-shock proteins and/or disruption of cellular signaling. METHODS: In Sprague-Dawley rats, the middle cerebral artery was occluded for 90 min using the intraluminal suture method. Geldanamycin (300 mg/kg) or vehicle was injected intraperitoneally 15 min before onset of ischemia or reperfusion. Animals were sacrificed at 2, 4 or 24 h after ischemia onset and brain samples were processed for infarct volume measurement, Western blot analysis or immunofluorescent staining of Hsp90, Raf-1, p38, and p44/42 mitogen-activated protein kinases (MAPKs). RESULTS: Geldanamycin treatment during ischemia or reperfusion reduced infarct volume by 79 and 61 % respectively. Geldanamycin decreased Raf-1 and activated p44/42 MAPK proteins, but did not alter levels of activated p38 MAPK during early reperfusion. Hsp90 was co-localized with Raf-1 and activated p44/42 MAPK in the cytoplasm of ischemic neurons. CONCLUSION: Geldanamycin-induced protection against transient focal cerebral ischemia may in part be based upon depletion of Raf-1 and blockade of p44/42 MAPK activation.


Subject(s)
Benzoquinones/therapeutic use , Infarction, Middle Cerebral Artery/therapy , Lactams, Macrocyclic/therapeutic use , Mitogen-Activated Protein Kinase 3/metabolism , Myocardial Reperfusion Injury/drug therapy , Neuroprotective Agents/therapeutic use , Analysis of Variance , Animals , Brain/drug effects , Brain/metabolism , Brain/pathology , Brain Infarction/prevention & control , Disease Models, Animal , Enzyme Activation/drug effects , Gene Expression Regulation, Enzymologic/drug effects , Male , Myocardial Reperfusion Injury/complications , Proto-Oncogene Proteins c-raf/metabolism , Rats , Rats, Sprague-Dawley
2.
Transl Stroke Res ; 3(1 Suppl 1): 180-7, 2012 Jul 01.
Article in English | MEDLINE | ID: mdl-23162674

ABSTRACT

Ischemic preconditioning (IPC) provides protection against subsequent severe ischemic injury. A recent study found that cerebral IPC prolongs bleeding time. In this study, we examined whether IPC protects against intra-cerebral hemorrhage (ICH)-induced brain edema formation and whether IPC affects blood coagulation. There were three sets of experiments in this study. In the first set, male Sprague-Dawley rats were preconditioned with either 15 min of left middle cerebral artery occlusion, an IPC stimulus, or a sham operation. Three days later, rats received an infusion of autologous whole blood in the ipsilateral or contralateral caudate. Rats were killed 24 h later for brain water content measurement. In the second set, rats underwent 15 min of IPC or a sham operation. Three days later, rats were used for bleeding and thrombin clotting time tests. In the third set, the levels of p44/42 mitogen-activated protein kinases (MAPKs), heme oxygenase-1 (HO-1), transferrin (Tf), and transferrin receptor (TfR) in the brain 24 or 72 h after IPC were examined. We found that IPC reduced ICH-induced brain edema when blood was injected into the ipsilateral caudate but it did not when blood was injected into the contralateral caudate. IPC resulted in prolongation of bleeding time and thrombin clotting time. IPC also induced the activation of p44/42 MAPKs and upregulation of HO-1, Tf, and TfR levels in the ipsilateral caudate. These results suggest that IPC protects against ICH-induced brain edema formation and decreases blood coagulation. The protection of IPC against ICH is mainly due to local factors in the brain and may be related to activation of p44/42 MAPKs and upregulation of HO-1, Tf, and TfR.

3.
Stroke ; 38(4): 1362-7, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17322079

ABSTRACT

BACKGROUND AND PURPOSE: An increased risk of hemorrhagic transformation is a major factor limiting the use of tissue plasminogen activator for stroke. Increased hemorrhagic transformation is also found in animals undergoing transient focal cerebral ischemia with hyperglycemia; this study examined whether hyperbaric oxygen (HBO) could reduce such hemorrhagic transformation in a rat model. METHODS: Rats received an injection of 50% glucose (6 mL/kg intraperitoneally) and had a middle cerebral artery occlusion 10 minutes later. Rats were treated with HBO (3 ATA for 1 hour) 30 minutes after middle cerebral artery occlusion. Control rats received normobaric room air. Rats underwent reperfusion 2 hours after middle cerebral artery occlusion. Blood-brain barrier permeability (Evans blue), hemorrhagic transformation (hemoglobin content), brain edema, infarct volume, and mortality were measured. RESULTS: HBO treatment reduced Evans blue leakage in the ipsilateral hemisphere (28.4+/-3.5 versus 71.8+/-13.1 microg/g in control group, P<0.01) 2 hours after reperfusion and hemorrhagic transformation (0.13+/-0.13 versus 0.31+/-0.28 mg hemoglobin in the control group, P<0.05) 22 hours later. Mortality was less in the HBO group (4% versus 27% in controls, P<0.05). Mean infarct volume and swelling in the caudate were also less in HBO-treated rats (P<0.05), but HBO failed to reduce brain water content in the ipsilateral hemisphere (P>0.05). CONCLUSIONS: Early intraischemic HBO treatment reduces the blood-brain barrier disruption, hemorrhagic transformation, and mortality after focal cerebral ischemia suggesting that HBO could be used to reduce hemorrhagic conversion in patients with stroke.


Subject(s)
Cerebral Hemorrhage/etiology , Cerebral Hemorrhage/prevention & control , Cerebral Infarction/complications , Hyperbaric Oxygenation/methods , Ischemic Attack, Transient/complications , Oxygen/pharmacology , Animals , Blood-Brain Barrier/drug effects , Blood-Brain Barrier/physiopathology , Cerebral Hemorrhage/physiopathology , Cerebral Infarction/physiopathology , Disease Models, Animal , Fibrinolytic Agents/adverse effects , Hemoglobins/metabolism , Infarction, Middle Cerebral Artery/complications , Infarction, Middle Cerebral Artery/physiopathology , Ischemic Attack, Transient/physiopathology , Male , Oxygen/therapeutic use , Rats , Rats, Sprague-Dawley , Reperfusion Injury/complications , Reperfusion Injury/physiopathology , Tissue Plasminogen Activator/adverse effects , Treatment Outcome
4.
Neurosurgery ; 57(3): 565-72; discussion 565-72, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16145537

ABSTRACT

OBJECTIVE: To develop a highly reproducible rat model and behavioral tests for acute subdural hematoma (ASDH) and to investigate the role of intravascular coagulation and thrombin in the pathogenesis of brain injury in this model. METHODS: A new method was implemented to inject 200 microl of autologous blood subdurally in rats. Immunohistochemistry was used to investigate intravascular fibrin deposition and thrombin levels in the cortex underlying the ASDH. Effectiveness of systemic heparin, argatroban, or ginkgolide B treatment was determined by histological lesion volume, number of occluded microvessels, and neurological deficits. Neurological deficits were monitored for 7 days after ASDH by use of forelimb placing, forelimb use asymmetry, and corner turn tests. RESULTS: Consistent brain damage and sensorimotor deficits were observed in all animals with ASDH. Histological analysis demonstrated occluded microvessels and enlarged perivascular spaces in the underlying cortex starting 1 hour after hematoma induction. Fibrin and thrombin immunoreactivity were increased in the lesioned cortical parenchyma at 4 and 24 hours. However, no intravascular fibrin deposition was detected. Heparin induced hemorrhagic transformation in the cortical lesion and did not attenuate microvessel occlusion. Argatroban and ginkgolide B did not induce hemorrhage but failed to improve microvessel occlusion, lesion volume, and neurological deficits. CONCLUSION: Intravascular coagulation and thrombin are not the major mediators of brain damage after ASDH. The model and behavioral tests presented in this study can be used to investigate other putative mechanisms of injury and to test future therapeutic interventions in ASDH.


Subject(s)
Anticoagulants/therapeutic use , Disease Models, Animal , Diterpenes/therapeutic use , Fibrinolytic Agents/therapeutic use , Hematoma, Subdural, Acute/drug therapy , Lactones/therapeutic use , Pipecolic Acids/therapeutic use , Animals , Arginine/analogs & derivatives , Behavior, Animal , Extremities/physiopathology , Ginkgolides , Hematoma, Subdural, Acute/pathology , Hematoma, Subdural, Acute/physiopathology , Male , Movement/drug effects , Movement/physiology , Psychomotor Performance/drug effects , Psychomotor Performance/physiology , Rats , Reproducibility of Results , Sulfonamides , Time Factors
5.
Eur J Nucl Med Mol Imaging ; 31(5): 733-9, 2004 May.
Article in English | MEDLINE | ID: mdl-14985868

ABSTRACT

In this study we wished to determine whether technetium-99m annexin V, an in vivo marker of cellular injury and death, could be used to noninvasively monitor neuronal injury following focal middle cerebral artery (MCA) occlusion/reperfusion injury. Sixteen adult male Sprague-Dawley rats (along with four controls) underwent left (unilateral) MCA intraluminal beaded thread occlusion for 2 h followed by reperfusion. One hour following tail vein injection of 5-10 mCi of (99m)Tc-annexin V, animals underwent either single-photon emission computerized tomography (SPECT) or autoradiography followed by immunohistochemical analyses. There was abnormal, bilateral, multifocal uptake of (99m)Tc-annexin V in each cerebral hemisphere as seen by both SPECT and autoradiography at 4 h and 1, 3, and 7 days after initiation of occlusion. The average maximal annexin V uptake at 4 h was 310%+/-85% and 365%+/-151% above control values (P<0.006) within the right and left hemispheres, respectively, peaking on day 3 with values of 925%+/-734% and 1,194%+/-643% (P<0.03) that decreased by day 7 to 489%+/-233% and 785%+/-225% (P<0.01). Total lesional volume of the left hemisphere was 226%, 261%, and 451% ( P<0.03) larger than the right at 4, 24, and 72 h after injury, respectively. Annexin V localized to the cytoplasm of injured neurons ipsilateral to the site of injury as well as to otherwise normal-appearing neurons of the contralateral hemisphere as confirmed by dual fluorescent microscopy. It is concluded that there is abnormal bilateral, multifocal annexin V uptake, greater on the left than on the right side, within 4 h of unilateral left MCA ischemic injury and that the uptake peaks at 3 days and decreases by 7 days after injury. This pattern suggests that neuronal stress may play a role in the response of the brain to focal injury and be responsible for annexin V uptake outside the region of ischemic insult.


Subject(s)
Annexin A5 , Hypoxia-Ischemia, Brain/diagnostic imaging , Neurons/diagnostic imaging , Organotechnetium Compounds , Reperfusion Injury/diagnostic imaging , Animals , Apoptosis , Cerebral Arteries/diagnostic imaging , Cerebral Arteries/injuries , Cerebral Arteries/pathology , Disease Models, Animal , Hypoxia-Ischemia, Brain/etiology , Hypoxia-Ischemia, Brain/pathology , Male , Neurons/pathology , Oxidative Stress , Radiopharmaceuticals , Rats , Rats, Sprague-Dawley , Reperfusion Injury/complications , Reperfusion Injury/pathology , Tomography, Emission-Computed, Single-Photon/methods
6.
J Cereb Blood Flow Metab ; 24(2): 159-66, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14747742

ABSTRACT

There has been considerable interest in the use of thrombin inhibitors to reduce the occurrence of stroke or to potentiate tissue plasminogen activator-induced reperfusion. However, there is growing evidence that thrombin may also have extravascular effects that influence ischemic brain injury. Male Sprague-Dawley rats were subjected to either 90 minutes of temporary middle cerebral artery (MCA) occlusion or sham operation to examine thrombin and protease activated receptor-1 (PAR-1) expression. In another set of rats, the MCA was occluded for 90 minutes and 10 U of hirudin or the same volume of vehicle was injected into the caudate followed by reperfusion for up to 28 days, to test the effects of local thrombin inhibition on ischemic damage, neurologic outcome and cerebral blood flow (CBF). Thrombin immunoreactivity was increased in the ischemic caudate at 4 and 24 hours, whereas PAR-1 expression was unchanged. Hirudin reduced infarct volume in the caudate at 24 hours (79 +/- 41 vs. 115 +/- 20 mm3, P < 0.05) and resulted in a larger residual tissue volume in the caudate at 28 days (17.6 +/- 3.9 vs. 11.8 +/- 6.3 mm3, P < 0.05). Hirudin treatment also had a beneficial effect on body weight and ameliorated neurologic deficits tested by forelimb placing and forelimb use asymmetry during 28 days survival. These beneficial effects of hirudin were not associated with improved regional CBF during reperfusion. These results suggest that, in addition to their effects on coagulation and circulation, thrombin inhibitors also have direct neuroprotective properties and may be considered in stroke therapy.


Subject(s)
Brain Ischemia/metabolism , Brain Ischemia/pathology , Cerebrovascular Circulation/physiology , Fibrinolytic Agents/metabolism , Hirudins/metabolism , Animals , Body Weight , Brain/anatomy & histology , Brain/metabolism , Brain/pathology , Infarction, Middle Cerebral Artery , Male , Microinjections , Motor Activity , Neuroprotective Agents/metabolism , Rats , Rats, Sprague-Dawley , Receptor, PAR-1/metabolism , Regional Blood Flow , Thrombin/metabolism
7.
J Neurosurg ; 98(6): 1271-6, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12816275

ABSTRACT

OBJECT: In this study the authors examined the influence of mild hypothermia on early expression of nitric oxide synthase (NOS) isoforms and peroxynitrite generation after experimental stroke. METHODS: In 82 male Sprague-Dawley rats, middle cerebral artery occlusion was performed for 2 hours by using the intraluminal suture model. The rats were maintained at their normal body temperature or exposed to 2 hours of intraischemic or postischemic (2-hour delay) mild hypothermia. Brains were collected 2, 6, and 24 hours after onset of ischemia for immunohistochemical and Western blot analysis of neuronal (n)NOS and inducible (i)NOS expression and peroxynitrite generation. CONCLUSIONS: Western blots showed significantly increased nNOS and iNOS expression in the ischemic cortex at 2, 6, and 24 hours compared with sham-operated animals. The NOS expression was highest at 24 hours. Postischemic hypothermia attenuated nNOS expression at 6 and 24 hours to a greater extent than intraischemic hypothermia. Intraischemic hypothermia reduced iNOS expression at both 2 and 24 hours, whereas postischemic hypothermia decreased iNOS expression at 24 hours. Results of immunohistochemical studies showed that nNOS colocalized with the neuronal marker MAP-2 at all time points, whereas iNOS was initially localized to vessels, and then localized to activated microglia by 24 hours. Intraischemic but not postischemic hypothermia decreased the number of nitrotyrosine-positive cells in the ischemic cortex at 24 hours. Mild hypothermia significantly but differentially attenuates increases in NOS isoforms, with more robust nNOS suppression when cooling is delayed. This may have important implications for understanding the mechanism of hypothermic neuroprotection and for stroke therapy.


Subject(s)
Brain Ischemia/enzymology , Brain Ischemia/pathology , Brain/enzymology , Brain/pathology , Hypothermia, Induced/methods , Nitric Oxide Synthase/metabolism , Animals , Blotting, Western , Immunohistochemistry , Isoenzymes , Male , Models, Animal , Neurons/enzymology , Nitric Oxide Synthase Type I , Nitric Oxide Synthase Type II , Rats , Rats, Sprague-Dawley , Time Factors
8.
J Cereb Blood Flow Metab ; 23(5): 589-98, 2003 May.
Article in English | MEDLINE | ID: mdl-12771574

ABSTRACT

Nuclear factor-kappaB (NFkappaB) is a transcription factor that is activated after cerebral ischemia. NFkappaB activation leads to the expression of many inflammatory genes involved in the pathogenesis of stroke. The authors previously showed that mild hypothermia is protective even when cooling begins 2 h after stroke onset. In the present study, they examined the influence of hypothermia on NFkappaB activation. Rats underwent 2 h of transient middle cerebral artery occlusion. Brains were cooled to 33 degrees C immediately after or 2 h after occlusion, and maintained for 2 h. After normothermic ischemia (brain temperature at 38 degrees C), NFkappaB cytoplasmic expression, nuclear translocation, and binding activity were observed as early as 2 h in the ischemic hemisphere and persisted at 24 h. Hypothermia decreased NFkappaB translocation and binding activity but did not alter overall expression. Hypothermia also affected the levels of NFkappaB regulatory proteins by suppressing phosphorylation of NFkappaB's inhibitory protein (IkappaB-alpha) and IkappaB kinase (IKK-gamma) and decreasing IKK activity, but did not alter overall IKK levels. Hypothermia suppressed the expression of two NFkappaB target genes: inducible nitric oxide synthase and TNF-alpha. These data suggest that the protective effect of hypothermia on cerebral injury is, in part, related to NFkappaB inhibition due to decreased activity of IKK.


Subject(s)
Hypothermia, Induced , Infarction, Middle Cerebral Artery/metabolism , Infarction, Middle Cerebral Artery/therapy , NF-kappa B/metabolism , Animals , Blotting, Western , Encephalitis/immunology , Encephalitis/metabolism , Encephalitis/therapy , Gene Expression , I-kappa B Kinase , I-kappa B Proteins/metabolism , Immunohistochemistry , Infarction, Middle Cerebral Artery/immunology , Male , NF-KappaB Inhibitor alpha , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase Type II , Phosphorylation , Protein Serine-Threonine Kinases/metabolism , Rats , Rats, Sprague-Dawley , Tumor Necrosis Factor-alpha/genetics
9.
J Neurosci ; 22(10): 3921-8, 2002 May 15.
Article in English | MEDLINE | ID: mdl-12019311

ABSTRACT

Mild hypothermia is neuroprotective, but the reasons are not well known. Inflammation contributes to ischemic damage; therefore, we examined whether the protection by hypothermia may be attributable to alterations in the inflammation. We examined whether hypothermia might alter the inflammatory cell-associated inducible nitric oxide synthase (iNOS) and subsequent nitric oxide (NO) and peroxynitrite generation in experimental stroke and inflammation. Rats underwent 2 hr of middle cerebral artery occlusion (MCAO). Brain inflammation was modeled by intravenous lipopolysaccharide (LPS) (2 mg/kg) injection. Temperature was maintained at 33 degrees C for 2 hr immediately after MCAO and LPS injection, delayed 2 hr after MCAO or maintained at 38 degrees C. Cultured microglia were activated with LPS and then incubated at 33 or 37 degrees C. Both intraischemic and delayed mild hypothermia attenuated infarct size by 40% (p < 0.05). Immunohistochemistry was performed to identify cell type, iNOS, and peroxynitrite. The majority of iNOS- and peroxynitrite-positive cells were activated microglia-macrophages, and mild hypothermia significantly decreased the numbers of immunoreactive cells at 72 hr by >50% (p < 0.05). After ischemia, mild hypothermia decreased NO production by 40%. Similarly, hypothermia attenuated NO and iNOS in LPS-injected rats, as well as in cultured microglia. Aminoguanidine, an iNOS inhibitor, also attenuated infarct size and NO in ischemic and inflammation models. We conclude that mild hypothermia significantly inhibits the inflammatory response by affecting microglial iNOS-NO generation. Therapies directed against microglia or their activation may be useful in treating stroke.


Subject(s)
Encephalitis/metabolism , Hypothermia, Induced , Nitric Oxide Synthase/metabolism , Reactive Nitrogen Species/biosynthesis , Stroke/metabolism , Tyrosine/analogs & derivatives , Animals , Cells, Cultured , Disease Models, Animal , Encephalitis/chemically induced , Encephalitis/pathology , Hypothermia, Induced/methods , Infarction, Middle Cerebral Artery/complications , Infarction, Middle Cerebral Artery/metabolism , Infarction, Middle Cerebral Artery/pathology , Lipopolysaccharides , Male , Microglia/cytology , Microglia/metabolism , Monocytes/metabolism , Monocytes/pathology , Nitric Oxide Synthase Type II , Rats , Rats, Sprague-Dawley , Stroke/complications , Stroke/pathology , Tyrosine/metabolism
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