ABSTRACT
Results of comparative immunoenzymatic study of matrix metalloproteinase (MMP) 2, 8 and 9, interleukins (IL) If and 6, tissue MMP inhibitors (TIMP-1and TIMP-2) and TNF-a in oral fluid of patients with different teeth and denture constructive materials show that MMP-9 content in oral fluid can serve as a marker of chronic generalized periodontitis because it is elevated in all patients irrespective of presence or absence of metallic tooth restorations. MMP-8 level is elevated as compared to control only in periodontitis patients with metallic restorations. The character of correlative relationships between the parameters studied in various patients'groups demonstrate relative similarity of MMP, IL and TIMP secretion regulation in patients with intact periodontal. In patients with inflammatory destructive periodontal lesions both with and without metallic restorations the correlation data reveal a cascade of biochemical reactions in response to etiologic factors. More pronounced response is observed in periodontitis patients with metallic orthopedic constructions. The presence of chromium-cobalt or chromium-nickel constructions leads to an increase of MMP-2, IL-lf and IL-6 content in oral fluid.
Subject(s)
Cytokines/metabolism , Dental Prosthesis , Matrix Metalloproteinases/metabolism , Periodontitis/metabolism , Saliva/metabolism , Tooth/metabolism , Adolescent , Adult , Case-Control Studies , Cytokines/genetics , Dentition, Permanent , Female , Humans , Male , Matrix Metalloproteinases/genetics , Metal Ceramic Alloys/chemistry , Metal Ceramic Alloys/pharmacology , Metals/pharmacology , Middle Aged , Saliva/drug effects , Tissue Inhibitor of Metalloproteinase-1/genetics , Tissue Inhibitor of Metalloproteinase-1/metabolism , Tissue Inhibitor of Metalloproteinase-2/genetics , Tissue Inhibitor of Metalloproteinase-2/metabolism , Tooth/drug effectsABSTRACT
We present the results of comparative enzyme-linked immunosorbent assay of matrix metalloproteinases MMP-2, MMP-8, MMP-9; IL-1ß and IL-6; tissue inhibitors of MMP (TIMP-1, TIMP-2), and TNF-α. The object of study was oral fluid of practically healthy subjects with intact periodontium and patients with chronic generalized periodontitis with various structural materials of dental restorations. It was found that MMP-9 in oral fluid could be considered as a marker of chronic generalized periodontitis irrespective of the presence or absence of metal dental restorations. The level of MMP-8 surpassed the normal only in oral fluid of patients with chronic generalized periodontitis with metal restorations. The correlations between the studied parameters in patients attest to relatively similar regulation of MMP, IL and TIMP secretion in oral fluid in practically healthy subjects with intact periodontium. In patients with inflammatory destructive periodontal diseases with and without metal dental restorations, the correlation coefficients indicate initiation of biochemical cascade accompanied by activation of cytokine production in response to etiological factors. Patients with periodontitis and metal orthodontic structures showed more pronounced reaction. Orthodontic structures made from chromium-cobalt, or chromium-nickel alloys in the oral cavity of these patients increased the levels of MMP-2, IL-1ß and IL-6 in oral fluid.
Subject(s)
Chronic Periodontitis/metabolism , Cytokines/metabolism , Gingival Crevicular Fluid/metabolism , Matrix Metalloproteinases/metabolism , Adolescent , Adult , Enzyme-Linked Immunosorbent Assay , Female , Humans , Interleukin-6/metabolism , Male , Matrix Metalloproteinase 8/metabolism , Matrix Metalloproteinase 9/metabolism , Middle Aged , Tissue Inhibitor of Metalloproteinase-1/metabolism , Tissue Inhibitor of Metalloproteinase-2/metabolism , Tumor Necrosis Factor-alpha/metabolism , Young AdultABSTRACT
The content of MMP-8 and MMP-9 in oral fluid of 145 patients (95 women and 50 men, 18-52 years) was measured by enzyme immunoassay. We examined 63 subjects with the intact periodontium and 82 patients with chronic generalized periodontitis (25 patients with mild disease, 45 patients with moderate disease, and 12 patients with severe disease). All patients were examined during the remission of chronic periodontitis and did not have clinical signs of associated somatic diseases. No significant differences were found in the content of MMP-8 and MMP-9 in oral fluid from periodontitis patients and subjects with the intact periodontium. The content of MMP-8 an MMP-9 in oral fluid of patients with severe periodontium was slightly higher than that in other patients and subjects with the intact periodontium. The depth of the periodontal pocket was shown to be the most reliable clinical sign for the state of periodontal tissues. A strong correlation was revealed between this criterion and MMP-9 content in oral fluid.