Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Database
Language
Publication year range
1.
Transbound Emerg Dis ; 58(2): 179-85, 2011 Apr.
Article in English | MEDLINE | ID: mdl-26353053

ABSTRACT

We investigated bovine coronavirus (BCoV) as an etiological agent in cattle with clinical respiratory and digestive signs using 147 feces and 199 nasal swab samples. A total of 18 test samples (16 feces and 2 nasal swap samples) were detected positive by ELISA and/or RT-PCR targeting the BCoV N gene. The partial S1 gene regions of BCoVs (An-4 and An-11) detected in feces samples from two herd-mate dairy calves were compared. Virological and serological results indicated that BCoVs are widespread in Turkey and are likely etiological agents in diarrhea cases in calves.


Subject(s)
Cattle Diseases/epidemiology , Coronavirus Infections/veterinary , Coronavirus, Bovine/genetics , Spike Glycoprotein, Coronavirus/genetics , Animals , Cattle , Cattle Diseases/virology , Coronavirus Infections/epidemiology , Coronavirus Infections/virology , Coronavirus, Bovine/isolation & purification , Coronavirus, Bovine/metabolism , Enzyme-Linked Immunosorbent Assay/veterinary , Feces/virology , Molecular Sequence Data , Nose/virology , Phylogeny , Sequence Analysis, DNA/veterinary , Spike Glycoprotein, Coronavirus/metabolism , Turkey/epidemiology
2.
Vet Microbiol ; 135(3-4): 374-9, 2009 Mar 30.
Article in English | MEDLINE | ID: mdl-19019578

ABSTRACT

Blood samples from sheep and/or goats from eight small ruminant flocks in the Turkish provinces of Aydin and Burdur were tested for the presence of Pestiviruses using an antigen-capture ELISA. From clinically affected animals, pathological and immunohistochemical findings were recorded. Post mortem examination of a virus-positive lamb showing abnormal fleece and paralysis of the hind legs revealed nonsuppurative meningoencephalomyelitis with hypomyelinogenesis. By immunohistochemistry Pestivirus antigen was detected in all parts of the brain including cerebellum, cerebral hemispheres and midbrain. Two Pestivirus isolates from a sheep and a goat kid, respectively, were isolated from samples that were positive in the antigen-capture ELISA. Genetic typing using the 5'-NTR (288bp) and N(pro) (738bp) showed that both were Border disease virus (BDV) isolates. By phylogenetic analysis, they formed a cluster clearly separated from the known clusters BDV-1 to BDV-6 and might therefore represent a new subgroup (BDV-7?). This is the first report confirming the occurrence and partial characterisation of BDV infection in small ruminants in Turkey.


Subject(s)
Border Disease/epidemiology , Border disease virus/pathogenicity , Animals , Antibodies, Viral/immunology , Antigens, Viral/analysis , Antigens, Viral/blood , Border disease virus/classification , Border disease virus/genetics , Cerebrum/virology , Genotype , Geography , Goat Diseases/blood , Goat Diseases/epidemiology , Goat Diseases/virology , Goats/virology , Hindlimb/virology , Pestivirus/genetics , Pestivirus/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , Sheep , Sheep Diseases/blood , Sheep Diseases/epidemiology , Sheep Diseases/virology , Turkey/epidemiology
3.
Dtsch Tierarztl Wochenschr ; 112(7): 271-4, 2005 Jul.
Article in English | MEDLINE | ID: mdl-16124702

ABSTRACT

Serum samples of 15,909 cattle from 31 dairy herds located in various regions of Turkey were tested for the presence of antibodies against bovine leucosis virus (BLV) using Agar Gel Immuno-diffusion technique (AGID). 48.3% (15/31) of the herds had seropositive animals and positivity rates were detected from 0.5-34.4% in these herds. In an EBL control/eradication programme all seropositive animals were culled in the infected herds. Thereafter, a total of 74,347 sera were tested for the presence of BLV specific antibodies. The serological results and detail of EBL control/eradication programme were shown in this paper.


Subject(s)
Antibodies, Viral/blood , Enzootic Bovine Leukosis/prevention & control , Leukemia Virus, Bovine/immunology , Age Factors , Animals , Cattle , Dairying/methods , Enzootic Bovine Leukosis/epidemiology , Female , Immunodiffusion/veterinary , Seroepidemiologic Studies , Turkey/epidemiology
4.
Acta Vet Hung ; 51(3): 425-31, 2003.
Article in English | MEDLINE | ID: mdl-14516168

ABSTRACT

In this study, bovine viral diarrhoea virus (BVDV) was detected and bio-typically characterised in clinical samples using reverse transcriptase-polymerase chain reaction (RT-PCR). The RT-PCR technique produced two different amplicons (402 and approx. 680 bp in size) in case of the presence of both biotypes (cp and ncp) in the sample. The mixture of the biotypes as detected by RT-PCR was verified by the immunoplaque assay (IPA). Purification of biotypes was carried out by native plaque isolation and subsequent RT-PCR revealed single products (402 or approx. 680 bp in size) in each clone. The results showed that RT-PCR can be used for accurate molecular differentiation between the BVDV biotypes.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/diagnosis , Diarrhea Viruses, Bovine Viral/genetics , Diarrhea Viruses, Bovine Viral/isolation & purification , RNA, Viral/analysis , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Animals , Bacterial Typing Techniques/veterinary , Cattle , Electrophoresis, Agar Gel/veterinary , Predictive Value of Tests
5.
J Oral Sci ; 43(2): 123-8, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11515596

ABSTRACT

There are numerous materials used to provide temporary coverage for teeth while permanent restorations are being fabricated. The biocompatibility of these materials is important for the protection of teeth and gingiva, but there is little information on this subject. This study aimed to examine possible time-dependent toxic effects of provisional crown and bridge restoration materials, manipulated intraorally or extraorally, on epithelia cells. A total of 20 discs, 4 sample discs from each product having dimensions of 4 mm diameter and 2 mm thickness, were prepared. After sterilization, toxicity of these discs was evaluated in the Madin Darby Bovine Kidney (MDBK) cell line. Morphological cell changes were observed microscopically at the 3rd, 6th, 12th, and 24th hours by the filter diffusion test method. After the 3rd hour, Artglass and Structur produced cytotoxic symptoms. Temdent had the least toxic effects at the end of the 12th hour. However, after the 24th hour, the toxic values were similar for all materials. The results of this study show that although the toxicity response of chemically and light curing materials had changed in different time periods, all of them had the same toxic effects at the end of the 24th hour.


Subject(s)
Crowns , Dental Materials/toxicity , Dental Restoration, Temporary , Denture, Partial, Temporary , Animals , Cattle , Cell Culture Techniques , Dental Cements/toxicity , Epithelial Cells/drug effects , Glass Ionomer Cements/toxicity , Kidney , Materials Testing , Methacrylates/toxicity , Methylmethacrylate/toxicity , Polymethyl Methacrylate/toxicity , Silicate Cement/toxicity , Time Factors , Toluidines/toxicity
6.
Dtsch Tierarztl Wochenschr ; 101(10): 390-1, 1994 Oct.
Article in English | MEDLINE | ID: mdl-7851298

ABSTRACT

In this study, a serological survey in goat herds for antibodies against Caprine Arthritis Encephalitis Virus (CAEV) in Turkey is described. 808 serum samples were collected from three state farms and seven private flocks in different regions of Turkey. The serologic examination was performed by Agar Gel Immunodiffusion (AGID) technique. 16 (1.9%) out of 808 sera deriving from two state farms (n = 14) and one private herd (n = 2) were found to be serologically positive. The findings are discussed with regard to the relatively low prevalence of CAEV in Turkey, age and race dependency and the kind of origin of the serologically screened animals.


Subject(s)
Antibodies, Viral/blood , Arthritis-Encephalitis Virus, Caprine/immunology , Goat Diseases/epidemiology , Lentivirus Infections/veterinary , Animals , Goats , Immunodiffusion/veterinary , Lentivirus Infections/epidemiology , Prevalence , Seroepidemiologic Studies , Turkey/epidemiology
SELECTION OF CITATIONS
SEARCH DETAIL
...