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2.
J Infect Public Health ; 13(4): 485-490, 2020 Apr.
Article in English | MEDLINE | ID: mdl-31838001

ABSTRACT

BACKGROUND: Nosocomial Clostridioides difficile infection (CDI) complicates up to 1% of all hospital admissions and is associated with considerable health burden. AIMS: To determine the incidence and outcomes of nosocomial CDI at a major University Medical Center. METHODS: Consecutive adult nosocomial CDI cases were prospectively identified. Stool samples were collected for ribotyping and antibiotic resistance testing. Patients were followed for eight weeks after discharge for relapse. RESULTS: Over a 2-year period, 215 patients developed nosocomial CDI (incidence 2:1000) and 200 (mean age 62.2±19.6 years) gave informed consent. Mean hospital stay was 23.3±28.9 days (range 0-278). Infection was diagnosed within 7 days of admission (range 0-95) in 129 patients (64.5%). More than two-thirds (69.0%) were previously hospitalized within 12 weeks of the index hospitalization. Twenty five percent received prior antibiotics within eight weeks. Fifty-two patients (26.0%) did not receive antibiotics prior to diagnosis. Considerable comorbidities (Charlson Comorbidity Index ≥8) were noted in 33.5% of patients. Recurrence occurred in 43 patients (21.5%). On multivariate logistic regression, fluoroquinolone exposure was the only predictor of recurrence (OR=2.9, 95%CI 1.1-7.7). Overall mortality was 14.0% and CCI ≥8 was the only predictor on multivariate analysis (p=0.004). Genotyping did not identify any known hypervirulent strains and all isolates were susceptible to metronidazole and vancomycin. CONCLUSION: Antibiotic exposure, comorbidities, and prior hospitalization constitute the major risk factors for nosocomial CDI. Recurrence is common and is associated with fluoroquinolones exposure. High baseline comorbidity score was the only predictor of increased mortality in this prospective cohort.


Subject(s)
Clostridium Infections/epidemiology , Cross Infection/epidemiology , Anti-Bacterial Agents/therapeutic use , Clostridioides difficile , Clostridium Infections/drug therapy , Clostridium Infections/etiology , Cross Infection/drug therapy , Cross Infection/etiology , Female , Humans , Incidence , Lebanon/epidemiology , Length of Stay/statistics & numerical data , Male , Middle Aged , Prospective Studies , Recurrence , Risk Factors , Treatment Outcome
3.
Int J Med Microbiol ; 308(3): 358-363, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29478838

ABSTRACT

Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on the pathogen's occurrence in other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. difficile isolates stemming from hospitalised patients with diarrhoea in Lebanon. From 129 stool samples of symptomatic patients at a tertiary care University hospital in Lebanon, a total of 107 C. difficile strains were cultivated and underwent ribotyping, toxin gene detection and antibiotic resistance testing. Ribotype 014 (RT014, 16.8%) predominated, followed by RT002 (9.3%), RT106 (8.4%) and RT070 (6.5%). Binary toxin gene-positive isolates (RT023, RT078 and RT126) were rarely detected and RT027 was absent. Interestingly, within one isolate only the toxin A gene (tcdA) was detected. Multiple-locus variable-number tandem repeat analysis (MLVA) revealed strong strain diversity in most RTs. The isolates were sensitive to metronidazole and vancomycin, and only a small proportion of strains displayed resistance against moxifloxacin, rifampicin, and clarithromycin (5.6%, 1.9%, and 2.8%), respectively. The data indicate that the genetic strain composition of Lebanese strains differs markedly from the situation seen in Europe and North America. Especially the epidemic RTs seen in the latter regions were almost absent in Lebanon. Interestingly, most strains showed almost no resistance to commonly used antibiotics that are suspected to play a major role in the development of C. difficile infection, despite frequent use of these antibiotics in Lebanon. Thus, the role of antimicrobial resistance as a major driving force for infection development remains uncertain in this area.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Toxins/genetics , Clostridioides difficile/drug effects , Clostridioides difficile/genetics , Clostridium Infections/microbiology , Drug Resistance, Multiple, Bacterial , Bacterial Toxins/isolation & purification , Clostridioides difficile/isolation & purification , Clostridioides difficile/pathogenicity , Clostridium Infections/epidemiology , Enterocolitis, Pseudomembranous/epidemiology , Enterocolitis, Pseudomembranous/microbiology , Enterotoxins/genetics , Feces/microbiology , Female , Fluoroquinolones/pharmacology , Humans , Lebanon/epidemiology , Male , Metronidazole/pharmacology , Microbial Sensitivity Tests , Moxifloxacin , Multilocus Sequence Typing/methods , Phenotype , Ribotyping/methods , Vancomycin/pharmacology
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