Trace element levels in serum and gastric mucosa in patients with Helicobacter pylori positive and negative gastritis.

BACKGROUND: Most trace elements are inhibited by Helicobacter pylori-infection, and variations in specific element levels are linked to the development of stomach cancer. This is the first study to show the relationship between serum and tissue concentrations of twenty-five trace elements and H. pylori infection status. This study purposed to define serum and tissue trace element levels of 25 healthy individuals with Helicobacter pylori-positive gastritis and Helicobacter pylori-negative gastritis and to reveal their relationship with the disease. METHODS: Study groups consisted of sixty-two patients with Helicobacter pylori-positive, thirty-seven patients with Helicobacter pylori-negative, and thirty healthy individuals. Serum and tissue concentrations of twenty-five elements (aluminum, boron, arsenic, barium, calcium, beryllium, copper, cadmium, iron, chromium, mercury, lithium, potassium, magnesium, sodium, manganese, nickel, phosphorus, lead, scandium, strontium, selenium, tellurium, titanium, zinc) were defined by inductively coupled plasma optical emission spectrometry. RESULTS: Except for copper, lithium, and strontium elements in serum samples, other trace elements differed significantly between the groups (p < 0.05). The serum chromium (p = 0.002), mercury (p = 0.001), boron (p < 0.001), and cadmium (p < 0.001) levels of H. pylori-negative gastritis and H. pylori-positive gastritis participants were significantly different, and their serum concentrations were less than 0.5 µ/l. Boron, barium, beryllium, chromium, lithium, phosphorus and strontium elements in tissue samples did not differ significantly between the groups (p > 0.05). Manganese, nickel, tellurium and titanium elements were not detected in tissue and serum samples. The mean concentrations of calcium, beryllium, chromium, iron, potassium, lithium, magnesium, scandium, and selenium were higher in the tissues of patients with H. pylori gastritis compared to healthy control tissues. Also, cadmium could not be detected in tissue samples. There was a significant difference between H. pylori-infected tissue and serum chromium levels (p = 0.001), with lower levels detected in tissue samples. CONCLUSION: This is the first study that we are knowledgeable of that reports the concentrations of twenty five elements in both serum and tissue samples, as well as the relationship between trace elements and Helicobacter pylori-infection status. Dietary adjustment is indicated as an adjunct to medical therapy to stabilize trace elements because Helicobacter pylori bacteria cause inflammation and impair element absorption in gastritis patients. We also think that this study will shed light on studies on the relationship between Helicobacter pylori-trace elements and serum-tissue/healthy serum-tissue trace element levels of patients with Helicobacter pylori gastritis.

APTMS-BCAD modified magnetic iron oxide for magnetic solid-phase extraction of Cu(II) from aqueous solutions.

Fe3O4@SiO2-3-aminopropyltrimethoxysilane-1,8-bis (3-chloropropoxy) anthracene-9,10-dione was synthesized as a new, sustainable, and environmentally friendly adsorbent for magnetic solid-phase extraction of Cu(II) from aqueous solutions. The structure of the adsorbent was characterized by FTIR, XRD, SEM, EDX, and TEM analysis. Optimum conditions for Cu(II) adsorption were determined as adsorbent dose 0.04 g, pH 5.0, contact time 120 min, and beginning concentration of 30 mg/L in the adsorption process. The adsorption capacity for Cu(II) ions was 43.67 mg/g and the removal efficiency was 84.72 percent. The Langmuir isotherm and the pseudo-second-order model fit the experimental data better. Adsorption was a spontaneous and endothermic process based on the obtained thermodynamic properties such as ΔG°, ΔH°, and ΔS°. The results showed that the sorbent has good selectivity in the presence of competing ions. The method was determined to be accurate and effective using real water samples and CRM.

Serum trace element levels of gastrointestinal cancer patients in an endemic upper gastrointestinal cancer region.

Gastrointestinal (GI) cancers are an important cause of the death rate in Turkey and in the world. Especially upper (esophageal and gastric) GI cancers are the leading types of cancer by 20-27% in Eastern Turkey. Trace elements are involved in many physiological and metabolic processes in the human body. Recent studies suggest that the increase or decrease of trace elements in the body may be related to the formation and development of many diseases such as cancers, diabetes mellitus and cardiovascular diseases etc. METHOD AND MATERIALS: In this study some trace elements; cadmium (Cd), cobalt (Co), Copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), nickel (Ni), lead (Pb), zinc (Zn) concentrations were investigated in the blood serums of esophageal, gastric and colon cancer patients and healthy controls in Van city area, an endemic upper (esophageal and gastric) GI region of Eastern Turkey. The analysis of the elements was realized with a flame atomic absorption spectrophotometer (FAAS). RESULTS: Mean serum levels of Cd and Ni in esophageal and gastric cancer patients were very significantly lower compared to controls (p < 0.001 and < 0.005). In addition, the mean serum Co level of esophageal cancer patients was very significantly lower than in controls and gastric cancer patients (p < 0.002). Also, the mean serum levels of Fe and Mn in all (esophageal, gastric and colon) cancer groups were very significantly lower than in controls (p < 0.001 and 0.002). Mean serum levels of Cu, Mg, Pb and Zn were not significantly different in all cancer patients compared to controls. CONCLUSION: We have detected the deficiency of five trace elements (Fe, Mn, Cd, Ni, Co) in esophageal, gastric and colon cancer patients. The deficiencies of trace elements may be due to malign over consumption of the nutrients and to the malnutrition of the advanced cancer patients. Nutritional therapy rich in trace elements should be considered as one of the strategies to fight against cancers.

Adsorption performance of activated carbon synthesis by ZnCl2, KOH, H3PO4 with different activation temperatures from mixed fruit seeds.

In this study, new activated carbons (ACs) were synthesized from a mixture of lemon and orange seeds (LOS) for toxic metal removal from aqueous solutions. Adsorbents have been produced by chemical activation with different activation agents (with H3PO4, ZnCl2 and KOH) and activation temperature (600°C, 700°C, 800°C). The elemental analysis, FT-IR, XRD and SEM analyses were performed to determine the characterization of the ACs. A series of batch adsorption experiments were done to research the influence of various parameters such as pH, adsorbent dosage, contact time, temperature, the complexing agents on the toxic metal ions (Cu(II), Cr(III) and Ni(II)) removal capacity of ACs. Adsorption equilibration time was 60 min, the adsorption capacity was 118.02 mg/g for Ni(II) ions, 146.03 mg/g for Cr(III) ions and 150.45 mg/g for Cu(II) ions. The adsorption of toxic metal ions was observed as a maximum at pH = 5 on AC-ZnCl2 (600°C) produced under N2 atmosphere. The adsorption process was fitted with a Langmuir isotherm model and a pseudo-second-order kinetic equation, showing the metal ions adsorption on AC was monolayer coverage. Thermodynamic studies show that the adsorption is endothermic and spontaneous in nature. AC produced from the LOS mixture has not been encountered in the literature. A simple, easy-to-apply method has been developed for a new adsorbent prepared from a mixture of LOS with environmentally friendly, easy to produce, reusable, cost-effective, non-toxic and with good adsorption-desorption capacity. Therefore, this study will contribute to the world economy in terms of environmental and wastewater cleaning.