ABSTRACT
A sample of patient's faeces containing virus-like particles (VLP) of 27-34 nm was obtained during an outbreak of hepatitis E in the Kirghiz SSR. The identity of the VLP to hepatitis E virus and etiological association with the disease were demonstrated by immune electron microscopy and infection of Macaca fascicularis monkeys. This isolate of hepatitis E virus is able to induce experimental infection in domestic piglets which was very similar to experimental hepatitis E in primates when infected orally, intravenously and by the combined routes. The clinical manifestations included acute biochemical and histological hepatitis, excretion of hepatitis E virus in faeces, icteric sclerae and skin, hepatitis virus presence in the material from mesenteric lymph nodes. Immunosuppression aggravated hepatitis E infection in piglets. Piglet-to-piglet transmission of hepatitis E virus was demonstrated. During passages of the virus in piglets a shortening of the incubation period and the absence of jaundice was observed.
Subject(s)
Hepatitis, Viral, Animal/etiology , Hepatitis, Viral, Human/microbiology , Swine Diseases/etiology , Animals , Feces/microbiology , Hepatitis Antibodies/blood , Hepatitis Viruses/immunology , Hepatitis Viruses/isolation & purification , Hepatitis Viruses/pathogenicity , Hepatitis, Viral, Animal/immunology , Hepatitis, Viral, Animal/microbiology , Hepatitis, Viral, Animal/pathology , Humans , Liver/pathology , Macaca fascicularis , Swine , Swine Diseases/immunology , Swine Diseases/microbiology , Swine Diseases/pathologySubject(s)
Hepatitis E/epidemiology , Age Factors , Altitude , Biomarkers/chemistry , Climate , Disease Outbreaks/statistics & numerical data , Feces/chemistry , Geography , Hepatitis Antibodies/analysis , Hepatitis B Core Antigens/immunology , Hepatitis B Surface Antigens/analysis , Hepatitis E/immunology , Hepatitis E/transmission , Hepatovirus/immunology , Humans , Immunoglobulin M/analysis , Kyrgyzstan/epidemiology , SeasonsSubject(s)
Arboviruses , Altitude , Climate , Ecology , Geography , Kyrgyzstan , Seasons , TemperatureABSTRACT
Districts of the Osh region were classified by a number of signs with the aid of a computer for the purpose of epidemiological prognosis of the foci of Crimean hemorrhagic fever (CHF). The results obtained were compared with the data of virological study for CHF in these districts. This work showed that there were future prospects in using the computer program for prognostication of infections with natural nidality with the aid of a computer.