ABSTRACT
Nitric oxide synthase (eNOS/NOS3) is responsible for the endothelial synthesis of nitric oxide (NO(â¢)). G894T polymorphism leads to the amino acid substitution from Glu298Asp that causes lower NOS3 activity and basal NO(â¢) production in NOS3 894T (298Asp) allele carriers compared with the GG homozygotes. NO(â¢) acts as an antioxidant protecting against Fenton's reaction which generates highly reactive hydroxyl radicals. Allelic variation of NOS3 may influence an individual's risk of laryngeal cancer (LC). In the current study we have examined the possible relationship between NOS3 G894T genotypes and various systemic oxidative damage markers such as protein carbonyl, advanced oxidation protein products, Cu, Zn-superoxide dismutase, thiol group fractions, and lipid hydroperoxides in LC patients. Genotyping was carried out by PCR-RFLP. In LC patients with TT genotype, Cu, Zn-superoxide dismutase activities and nonprotein thiol levels were significantly higher than the controls. In patients with GT and GG genotype, high levels of lipid hydroperoxides showed statistical significance when compared to controls. Our results indicate a potential relationship among G894T polymorphism of NOS3, and impaired redox homeostasis. Further studies are required to determine the role of NOS3 gene polymorphism and impaired plasma redox homeostasis.
Subject(s)
Biomarkers, Tumor , Genotype , Laryngeal Neoplasms , Nitric Oxide Synthase Type III , Oxidative Stress , Polymorphism, Genetic , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolism , Case-Control Studies , Female , Humans , Laryngeal Neoplasms/enzymology , Laryngeal Neoplasms/genetics , Male , Neoplasm Proteins/genetics , Neoplasm Proteins/metabolism , Nitric Oxide Synthase Type III/genetics , Nitric Oxide Synthase Type III/metabolismABSTRACT
OBJECTIVE: The objective of this study was to evaluate the relationship between disease severity and biochemical parameters such as pentraxin3, CRP, fetuin-A, insulin and HOMA-IR levels in patients with psoriasis. PATIENTS AND METHODS: This study included 58 patients with psoriasis and 30 healthy controls admitted to Ankara Numune Teaching and Research Hospital between January 2011-August 2012. Serum pentraxin3, CRP, fetuin-A and insulin concentrations were determined. Also, HOMA-IR values were calculated. RESULTS: The serum values for CRP, insulin, HOMA-IR, pentraxin-3 and fetuin-A in patients with psoriasis were elevated than control subjects (p values=0.002, 0.003, 0.003, 0.006 vs 0.007, respectively). According to the PASI score, patients were divided into three groups, minimal, moderate and severe psoriasis. There were positive correlation between the levels of CRP and insulin, HOMA-IR, PASI score. In addition, PASI score values were positively correlated with insulin, HOMA-IR and fetuin-A levels. CONCLUSIONS: Elevated levels of pentraxin3, CRP, fetuin-A, insulin and HOMA-IR might play a role in the pathogenesis of psoriasis. Higher CRP, fetuin-A, insulin and HOMA-IR concentrations were associated with the severity of the psoriasis.
