ABSTRACT
The objective was to evaluate the gamete laboratory parameters that can to determine the possibility of achieving pregnancy in in vitro fertilization (IVF). A prospective study was carried in 196 patients undergone to conventional and oocyte donation IVF. After the evaluation of oocytes, embryos and the pregnancies was established that postmature oocytes were more frequently retrieved from patients that did not get pregnant. A good embryo morphology and principally the transfer of 4-cell embryos are common events in IVF. However, there were not significant difference in embryo parameters, number of blastomeres and embryo quality, between pregnant and not pregnant patients. In conclusion, it appear that bad oocyte quality has a negative effect over the possibility of achieving pregnancy. The number of blastomeres and embryo quality were not predictive of the possibility to get pregnant.
Subject(s)
Clinical Laboratory Techniques , Fertilization in Vitro/methods , Oocytes/cytology , Pregnancy Outcome , Pregnancy/physiology , Adult , Embryo Transfer , Female , Humans , Prospective StudiesABSTRACT
A comparative analysis of potentially functional spermatozoa per ejaculate, progressive motility, hypo-osmotic swelling test, acrosome integrity and sperm viability (24 and 48 h) was carried out in a group of 40 subfertile patients with varicocele and marginal semen analysis and 40 fertile subjects, in order to identify subclinical abnormalities that may explain subfertility. Patients with varicocele had lower numbers of potentially functional spermatozoa per ejaculate, progressive motility, acrosome and membrane integrity and sperm viability. These abnormalities were not related to the grade of varicocele, testicular volume or serum FSH concentration. A positive correlation between the hypo-osmotic swelling test and progressive motility (r = 0.71) and between potentially functional spermatozoa and the hypo-osmotic swelling test (r = 0.69) was found in patients with varicocele. These data suggest that some of the deleterious effects produced by the varicocele might be related to sperm migration and viability in the female genital tract and others to sperm-zona interaction and/or sperm-egg fusion.
Subject(s)
Infertility, Male/physiopathology , Semen , Spermatozoa , Varicocele/physiopathology , Adult , Female , Follicle Stimulating Hormone/blood , Humans , Male , Semen/cytology , Sperm MotilityABSTRACT
The obstetrical outcome of 47 pregnancies which was achieved by assisted reproduction techniques was analyzed. 38 were achieved by GIFT and 9 by IVF-ET. In both series we found that the analyzed parameters are similar to those described by other groups. But in discordance with Angle-Saxon groups, in Latino-American series the principal delivery way was by cesarean section.
