Diversity of ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes.

Diversity of Ukrainian winter common wheat varieties was studied with respect to the storage protein loci Gli-A1, Gli-B1, Gli-D1, Glu-A1, Glu-B1, Glu-D1, Gli-A3, Gli-B5, and Gli-A6 (362 varieties) and markers for the Lr34/Yr18/Pm38/Sr57/Bdv1 gene conferring moderate resistance to a number of biotrophic pathogens, the Tsn1 gene for sensitivity to the toxins A of the necrotrophic fungi Pyrenophora triticirepentis and Stagonospora nodorum, the Tsc2 gene for sensitivity to the toxin B of P. triticirepentis, and the TDF_076_2D gene for moderate resistance to Fusarium head blight (181 varieties). Significant differences in frequencies of alleles at these marker loci between groups of varieties developed in different soil and climatic zones were revealed. The retention of a set of predominant alleles of a certain zone in different periods of breeding was confirmed. At the same time, the appearance of new allele associations in the groups of varieties of the Steppe (in particular Gli-A1g and Glu-B1al) and the Central Forest-Steppe (1AL/1RS and Glu-B1d) in the last two decades has been noted. Nonrandom associations between alleles of disease resistance genes as well as alleles of disease resistance genes and storage protein alleles were revealed

[Allelic state of the molecular marker for the golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world cultivars of potato (Solanum tuberosum ssp. tuberosum)].

The purpose of our investigation was determination of allelic state of the H1 resistance gene against the pathotypes Ro1 and Ro4 of golden potato cyst nematode (Globodera rostochiensis) among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars. The allelic condition of the TG689 marker was determined by PCR with DNA samples isolated from tubers of potato and primers, one pair of which flanks the allele-specific region and the other one was used for the control of DNA quality. Among analyzed 77 potato cultivars the allele of marker associated with the H1-type resistance was found in 74% of Ukrainian and 90% foreign ones although some of those cultivars proved to be susceptible to the golden potato nematode in field. The obtained data confirm the presence of H1-resistance against golden nematode pathotypes Ro1 and Ro4 among the Ukrainian potato cultivars and efficiency of the used marker within the accuracy that has been declared by its authors.

[The efficacy of hepatotropic agent Remaxol in oncological patients with postoperative liver dysfunction].

The study of the efficiency and safety of the hepatotropic drug Remaxol in oncological patients with minimal, subclinical manifestations of liver dysfunction in the postoperative period was performed. It is shown that using of Remaxolcontributes to leveling the biochemical liver dysfunction: decrease of the concentration of total bilirubin, a decrease serum activity of lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. The unwanted effects of Remaxol were not identified.

[Concurrent chemoradiotherapy with moderate dose brachytherapy as remission induction for patients surgically treated for esophageal cancer].

The locally advanced cancer of thoracic esophagus was treated by induction chemo-irradiation therapy (IHRT) with intraluminal medium dose-rate brachytherapy (IMBT). From July 2009 to February 2012 twenty five patients (mean age 54.3 +/- 1.3 years) were included in the study. The length of the primary tumor was up to 6 cm in 10 pts and more than 6 cm in 15 pts. Induction therapy consisted of three IMBT sessions (7 Gy X 3; q7d) and two cycles of chemotherapy (PF; q28d), 26-31 days later the surgery was performed. Subtotal resection of the esophagus type of I Lewis was performed in 23 pts, transtracheal extirpation of the esophagus was performed in 2 pts.In all the cases 3F lymphodissection was performed. After IHRT in 15 of 25 pts. was obtained complete or partial response, in 10 of 25 pts was obtained disease stabilization. Perioperative complications occurred in 17 (68%) patients receiving conservative treatment, one patient (4%) died of treatment complications. In 6 cases (24%) morphology had shown the complete regression of primary tumor. Thus, the combination of intraluminal medium dose-rate brachytherapy and chemotherapy lead to reduction of primary tumor local spread and can be an efficient factor in improving the results of surgical treatment.

[Perspectives of development in anesthesiology and intensive care in oncology].

Despite of anaesthesiology and intensive care progress in oncology there are some problems that need to be solved. These include low individual significance of perioperative risk scale, the lack of approaches for postoperative correction of risk value, estimation of surgical stress value, approach to detection of pain mechanisms etc. The studies which will resolve these problems allow improving the quality of medical care for cancer patients.

[Identification of allelic state of leaf rust-resistance Lr34 gene in the cultivars of soft winter wheat of Ukrainian breeding].

The distribution of alleles at the Lr34 locus associated with leaf rust resistance among winter common wheat (Triticum aestivum L.) cultivars of Ukrainian breeding was studied. Co-dominant molecular-genetic marker cssfr5 was used for detection of the allelic condition of the Lr34 locus. The cultivars with detected allele Lr34(+) were identified as potentially "resistant" and the cultivars in which detected allele Lr34(-) were identified as potentially "susceptible". The collection of the cultivars (81 ones), created in the main plant breeding centers of Ukraine was analyzed. The allele Lr34(+) was identified in 44% cultivars. Results were compared with the data about the distribution of the Lr34(+) in cultivars created in different countries.

[Influence of intraoperative sodium adenosine triphosphate infusion on leukocyte dynamics following colonic resection in cancer patients].

The paper evaluates effects of different methods of systemic intraoperative anesthesia on the dynamics of leukocytes following colonic resection in tumor patients. Lymphocytes levels rose on day 2 after operation in patients receiving non-selective purine receptor agonists (sodium adenosine triphosphate) as an intraoperative analgesic and were significantly higher than in controls treated with conventional drugs (p

[Purine analgesia: experimental results and clinical perspectives].

The high rate of dissatisfaction with analgesia in patients undergoing surgery and by those with chronic pain prompts a search for new pain relief methods. Purine analgesia may considerably ease suffering in these patient groups. This review considers the results of the studies dealing with the analgesic activity of non-selective purine receptor agonists, such as adenosine and adenosine triphosphate, on experimental models and the feasibilities of their clinical application. The paper points to the experimental studies, the results of which are the basis for the clinical use of purines. Much attention is given to the studies using purine analgesia as a component of general anesthesia. It is no less than important that good results of treatment were obtained after administration of purine agonists to patients with neuropathic pain syndromes. Thus, purine analgesia should be regarded as a promising line of the development of anesthesiology.

Experimentally induced ischaemic pain in healthy humans is attenuated by the adenosine receptor antagonist theophylline.

AIMS: Endogenous adenosine is considered a prominent pain mediator in ischaemia. In contrast, it has been shown that exogenous adenosine can reduce tourniquet induced ischaemic pain in healthy volunteers. The aim of this study was to investigate if pharmacological antagonism of endogenous adenosine actions with an intravenous infusion of theophylline could attenuate experimentally induced ischaemic pain. METHODS: Nineteen healthy volunteers, 11 males, eight female, received theophylline 7 mg kg-1 or placebo intravenously, in a randomized, double blind and crossover fashion, prior to a sub-maximum effort forearm tourniquet test. Experiments were carried out with 1-week intervals to avoid pre-conditioning. Pain scores [visual analogue scale (VAS), 0-100] were assessed every minute up to a maximum of 30 min. RESULTS: The sum of pain scores (accumulation of VAS scores) was attenuated by theophylline, 691 [200-1550 (median and 25-75% percentiles)], compared with placebo, 1231 (545-1675), P < 0.001. Also, peak VAS pain was lower during theophylline treatment, 48 +/- 38 (mean +/- SD), compared with placebo, 74 +/- 27, P < 0.001. Blood pressure increased during the experiment with no difference between treatments. Heart rate was not affected by tourniquet or drug treatment. CONCLUSIONS: It is concluded that the adenosine receptor antagonist theophylline is able to attenuate the development of ischaemia pain during experimental ischaemia in humans. This implies a role for adenosine as both facilitatory mediator and a modulator of ischaemia skeletal muscle pain.

[Rheography of extracorporeal circulation: verification of method and calibration of the monitor]. / Reografiia iskusstvennogo krovoobrashcheniia: proverka metoda i kalibrovka monitora.

Cardiopulmonary bypass as a reference flow model for impedance cardiography monitoring is described in 34 children during open-heart surgery. Such an approach not only proved the accuracy and precision of impedance cardiac output measurement, but also gave a possibility to make intra-operative calibration of any cardiac output monitor which maintains its accuracy during the post-bypass period.

[Thermodilution and rheography: the coincidence and reproducibility of results]. / Termodiliutsiia i reografiia: sovpadenie i vosproizvodimost' rezul'tatov.

A comparison of the pulmonary artery thermodilution and tetrapolar impedance cardiography of cardiac output measurements based on 312 dual determinations has shown a good general coincidence, but better reproducibility of the impedance cardiography data and more evident character of the errors and artifacts. The authors' analysis of the mechanisms and causes of such phenomena is based both on their own conclusions and on a wide spectrum of the data of previous investigations.

[Rheographic monitoring of intraoperative hemodynamics]. / Reograficheskii monitoring intraoperatsionnoi gemodinamiki.

The authors summed up the experiences with using noninvasive monitoring of hemodynamics during 420 operations. The method was based on computed procession in the real time of the integral rheogram of the body. The advantages, shortcomings and technological details of the method were analyzed.