Effect of three-beam interference on phase-generated carrier demodulation of a fiber-optic interferometric sensor.

Homodyne demodulation using a phase-generated carrier (PGC) has been applied in fiber-optic interferometric sensors to overcome the signal fading and distortion due to the drift of the operating point. An assumption needed for the PGC method to be valid is that the sensor output is a sinusoidal function of the phase delay between the arms of the interferometer, which is readily achieved by a two-beam interferometer. In this work, we theoretically and experimentally study the effect of three-beam interference, whose output deviates from a sinusoidal function of the phase delay, on the performance of the PGC scheme. The results show that the deviation could lead to additional undesirable terms in the in-phase and quadrature components in PGC implementation, which may result in significant signal fading with the drift of the operating point. The theoretical analysis leads to two strategies for eliminating these undesirable terms so that the PGC scheme is valid for three-beam interference. The analysis and the strategies were validated experimentally using a fiber-coil Fabry-Perot sensor with two fiber Bragg grating mirrors, each having a reflectivity of 26%.

Modified phase-generated carrier demodulation of fiber-optic interferometric ultrasound sensors.

We propose and demonstrate a modified phase-generated carrier (PGC) demodulation scheme optimized for detection of ultrasound using interferometric sensors with sinusoidal fringes. The sensor used in demonstration is made from a pair of weak fiber Bragg-gratings at the ends of a coiled fiber that form a low-finesse Fabry-Perot interferometer. The phase of the laser source is modulated using an electro-optic phase modulator to generate the carrier signal and obtain 2 quadrature (the sine and cosine) terms at the first and the second order carrier frequencies. The signal of interest (ultrasound) has much higher frequency than the environmental perturbation but a very small amplitude that causes only small phase shift. Using small-signal approximation, for each of the 2 quadrature terms, we separate the contributions from the environmental perturbations (quasi-DC component) and from the ultrasound (AC component). The AC components that contain the information of the ultrasound signal are then further amplified with a large gain. The signal of interest is constructed by simple algebraic operations on the 2 quasi-DC components and the 2 amplified AC components involving multiplying and summing. This work provides a simple and robust demodulation method with potentially high sensitivity for fiber-optic interferometric ultrasound sensors.

Optothermal microbubble assisted manufacturing of nanogap-rich structures for active chemical sensing.

Guiding analytes to the sensing area is an indispensable step in a sensing system. Most of the sensing systems apply a passive sensing method, which waits for the analytes to diffuse towards the sensor. However, passive sensing methods limit the detection of analytes to a picomolar range on micro/nanosensors for a practical time scale. Therefore, active sensing methods need to be used to improve the detection limit in which the analytes are forced to concentrate on the sensors. In this article, we have demonstrated the manufacturing of nanogap-rich structures for active chemical sensing. Nanogap-rich structures are manufactured from metallic nanoparticles through an optothermally generated microbubble (OGMB) which is a laser-induced micron-sized bubble. The OGMB induces a strong convective flow that helps to deposit metallic nanoparticles to form nanogap-rich structures on a solid surface. In addition, the OGMB is used to guide and concentrate analytes towards the nanogap-rich structures for the active sensing of analytes. An active sensing method can improve the detection limit of chemical substances by an order of magnitude compared to a passive sensing method. The microbubble assisted manufacturing of nanogap-rich structures together with an active analyte sensing method paves a new way for advanced chemical and bio-sensing applications.

Print metallic nanoparticles on a fiber probe for 1064-nm surface-enhanced Raman scattering.

This Letter presents 1064-nm surface-enhanced Raman scattering (SERS) on an optical fiber probe, or 1064-nm-SERS-on-fiber. Metallic nanoparticles are printed on an optical fiber probe by using optothermal surface bubbles under ambient conditions. An optothermal surface bubble is a laser-induced micro-sized bubble that is formed on a solid-liquid interface. The SERS activity of the optical fiber probe for 1064-nm Raman microscopy is tested with rhodamine 6G in aqueous solution. The 1064-nm-SERS-on-fiber can reduce the fluorescent background noise that commonly exists in other Raman systems. It can also compensate for the decreased Raman signal due to the use of an infrared Raman laser. The 1064-nm-SERS-on-fiber will find potential applications in low-background-noise biosensing and endoscopy.

Optimization and Structural Stability of Gold Nanoparticle-Antibody Bioconjugates.

Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts have focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate to detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, and an understanding of bioconjugate stability is needed. Here, we show a method for optimizing Listeria monocytogenes polyclonal antibodies bioconjugation mechanisms to AuNPs via covalent binding at different pH values, from 2 to 11, and 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid, NaOH, HCl conditions. By fitting Lorentz curves to the amide I and II regions, we analyze the stability of the antibody secondary structure. This shows an increase in the apparent breakdown of the antibody secondary structure during bioconjugation as pH decreases from 7.9 to 2. We find variable adsorption efficiency, measured as the percentage of antibody adsorbed to the AuNP surface, from 17 to 27% as pH increases from 2 to 6 before decreasing to 8 and 13% at pH 7.9 and 11, respectively. Transmission electron microscopy (TEM) analysis reveals discrepancies between size and morphological changes due to the corona layer assembly from antibody binding to single nanoparticles versus aggregation or cluster self-assembly into large aggregates. The corona layer formation size increases from 3.9 to 5.1 nm from pH 2 to 6, at pH 7.9, there is incomplete corona formation, whereas at pH 11, there is a corona layer formed of 6.4 nm. These results indicate that the covalent binding process was more efficient at lower pH values; however, aggregation and deactivation of the antibodies were observed. We demonstrate that optimum bioconjugation condition was determined at pH 6 and MES buffer-type by indicators of covalent bonding and stability of the antibody secondary structure using Fourier transform-infrared, the morphological characteristics and corona layer formation using TEM, and low wavelength shifts of ultraviolet-visible after bioconjugation.

Fabricated nanogap-rich plasmonic nanostructures through an optothermal surface bubble in a droplet.

A rapid and cost-effective method for the fabrication of nanogap-rich structures is demonstrated in this Letter. The method utilizes the Marangoni convection around an optothermal surface bubble inside a liquid droplet with a nanoliter volume. The liquid droplet containing metallic nanoparticles reduces the sample consumption and confines the liquid flow. The optothermal surface bubble creates a strong convective flow that allows for the rapid deposition of the metallic nanoparticles to form nanogap-rich structures on any substrate under ambient conditions. This method will enable a broad range of applications such as biosensing, environmental analysis, and nonlinear optics.

Single-molecule detection at high concentrations with optical aperture nanoantennas.

Single-molecule detection has become an indispensable technology in life science, and medical research. In order to get meaningful information on many biological processes, single-molecule analysis is required in micro-molar concentrations. At such high concentrations, it is very challenging to isolate a single molecule with conventional diffraction-limited optics. Recently, optical aperture nanoantennas (OANs) have emerged as a powerful tool to enhance the single-molecule detection under a physiological environment. The OANs, which consist of nano-scale apertures on a metallic film, have the following unique properties: (1) nanoscale light confinement; (2) enhanced fluorescence emission; (3) tunable radiation pattern; (4) reduced background noise; and (5) massive parallel detection. This review presents the fundamentals, recent developments and future perspectives in this emerging field.

Optical fiber-based sensor for in situ monitoring of cadmium sulfide thin-film growth.

This work presents a scheme for in situ monitoring of thin-film growth. A fiber-optic sensor based on Fabry-Perot interferometric technique has been established for the first time to monitor in situ growth of thin films. This was applied for determining thickness of cadmium sulfide (CdS) thin films during growth. The fabrication process of CdS film was carried out in 30 mM cadmium acetate and thioacetamide solution at 60°C temperature. The estimated thickness determined during the growth was verified by scanning electron microscopy. This study shows that in situ measurement of the thickness of thin films is feasible by this new technique, and a close match of the estimated thickness was achieved.