ABSTRACT
The cytotoxic activity of four cyclometalated platinum(II) complexes [PtMe(vpy)(L)], containing 2-vinylpyridine (vpy) and the phosphine ligands (L) PMe2Ph (1a), PPh3 (1b), PMePh2 (1c), and P(c-Hex)3 (1d), were evaluated against human breast cancer (MDA-MB-231), human lung cancer (A549), human colon cancer (SW1116), and non-tumor epithelial breast (MCF-10 A) cell lines. The highest activity was found for 1c with IC50 values of 21.10 µM, 23.36 µM, and 12.96 µM, compared to cisplatin, which was 10.12 µM, 47.57 µM, and 19.50 µM against the A549, SW1116, and MDA-MB-231 cell lines, respectively. 1a-d showed a higher selectivity index (SI) than cisplatin. Docking studies confirmed interaction to the DNA minor groove for all complexes. Genotoxicity studies on 1c showed interactions with the genomic content of malignant cells. Compared with cisplatin as a positive control, a slight shift was found in the electrophoresis mobility, which was utilized further to study the direct interaction of 1c with DNA.
Subject(s)
Antineoplastic Agents , Coordination Complexes , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Cisplatin , Coordination Complexes/chemistry , DNA/chemistry , Humans , Platinum/chemistry , PyridinesABSTRACT
In designing high-performance, lightweight components, cellular structures are one of the approaches to be considered. The present study aimed to analyze the effect of the infill line distance of 3D printed circular samples on their compressive elastic behavior. Lightweight cellular poly-lactic acid (PLA) samples with a triangular infill pattern were exposed to cyclic compressive loading and their stiffness was investigated. PLA is one of the most commonly used thermoplastic materials in additive manufacturing using the fused filament fabrication (FFF) process. Cylindrical samples with a diameter of 11.42 mm and a height of 10 mm were printed using FFF technology with two different infill line distances (1.6 mm and 2.4 mm). Comparing the nominal compressive stress-nominal strain curves under cyclic loading showed that the first cycle response was significantly different with respect to the subsequent ones. Furthermore, an analysis of the dependence of the modulus of elasticity on the effects of cyclic loading was performed. It was found that through elastic deformation, and combined elastic and plastic deformation, the samples' properties such as stiffness could be altered.
