ABSTRACT
The tomato is one of the most important vegetable crops. The successful development of tomato cultures in Kazakhstan depends on the implementation of intensive agricultural methods, including breeding and selecting for new tomato varieties resistant to plant pathogens. Common tomato viruses, although not detected in our country to date, may potentially have a deleterious impact on agriculture if allowed to spread. The implementation of tomato breeding programs based on molecular markers of resistance is therefore an important preventive measure for protecting the agriculture and food safety of Kazakhstan. In the present work, we used nine molecular markers associated with resistance to three tomato viruses, i.e., tomato mosaic virus (ToMV), tomato spot wilt virus (TSWV), and tomato yellow leaf curl virus (TYLCV), to test the local breeding collection for the presence of genetic resistance factors. Two tomato varieties, 'Zhiraf' (Russia) and 'Sunnik' (Armenia), were revealed to possess the resistant allele marker PrRuG86-151 against ToMV; three hybrid forms had the same allele in the heterozygous state. One hybrid, based on the 'Mirsini' F1 variety from the Netherlands, had resistance to TSWV, which was confirmed by four markers: NCSw003, NCSw007, NCSw011, and NCSw012. Two cultivars, 'Nicola' and 'Malinovyi Slon' (Russia), and the local hybrid based on 'Yarkiy Rumyanets' had two to three resistant alleles of markers based on locus Tm-3 of resistance to TYLCV. The obtained results have demonstrated that the collection of tomato varieties involved in breeding programs in Kazakhstan lacks well-known genetic resistance factors to the considered tomato viruses. Thus, the prospective breeding programs require introduction of known resistant genetic resources to establish resistance to viruses using marker-assisted selection.
ABSTRACT
BACKGROUND AND AIM: Milk producers need to ensure that their cows are producing high-quality, nutritional milk, which is influenced by the breed, age, nutrition, and health status of lactating animals. The aim of this study was to determine the effect of animal breed, season, and milk production on the physicochemical characteristics of milk and predicting the effect of these changes on the composition and quality of milk products. MATERIALS AND METHODS: In total, 80 milk samples (40 Simmental and 40 Holstein) were analyzed from LLP "Kirova" of Pavlodar region (Simmental breed) and JSC "Astana-Onim" of Akmola region (Holstein breed) in the Republic of Kazakhstan. The physicochemical parameters, including fatty acid (FA) content, were studied. RESULTS: The Simmental cows had the lowest mass fraction of fat in the spring at 3.94% and the highest fat content in the winter at 4.09%, which was the overall highest fat content measured in both breeds (p<0.001). The lowest protein in the Simmentals was also measured in the spring. The Holstein cows had the highest fat content at 3.8% and the highest protein content at 3.3% in autumn. CONCLUSION: It was found that the season and breed significantly affected the physicochemical parameters and the FA contents in cow milk. The superiority of the Simmental cows over the Holstein cows throughout the year was statistically significant.
ABSTRACT
The main objective of dairy farming is to obtain highly productive animals that produce milk with high protein content and have good technological properties. Currently, with the development of molecular genetics and molecular biology, it is possible to identify genes directly or indirectly associated with the milk production of animals. The identification of variants of such genes that are preferable from the standpoint of selection will allow, apart from the conventional selection of animals, to perform selection at the level of deoxyribonucleic acid technologies, i.e. according to genotype. The paper includes the study of milk productivity indicators and physicochemical quality indicators of milk from cows of Simmental and Alatau breeds by loci of the kappa casein gene, and also determines the cheese suitability and technological properties of milk of cows of Simmental and Alatau breeds with different genotypic affiliations for kappa casein for the production of semi-hard cheeses. The determination of polymorphism by the kappa casein gene was carried out with the use of the polymerase chain reaction method for genotyping of the major milk proteins of kappa casein gene. In a comparative aspect, the interrelation of genotypes with milk productivity was studied, the frequencies of allelic polymorphism of cows of Simmental and Alatau breeds in the Republic of Kazakhstan were identified, the nature of milk productivity was determined for each genotype. Scientifically based data on the assessment of biological value, technological properties, cheese suitability of milk of cows of Simmental and Alatau breeds are obtained.
