Effect of brassinolide on tyrosine phosphorylation of pea leaf proteins.

Brassinosteroid-induced phosphorylation of tyrosine residues in proteins was studied. Proteins of crude extract of pea leaves were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed 7 and 13 tyrosine-phosphorylated proteins, respectively. Brassinolide increased the phosphorylation level of most of these proteins. With inhibitors of tyrosine protein phosphatases, such as phenylarsine oxide and orthovanadate, the level of tyrosine phosphorylation of these proteins increased.

[Effect of antioxidant Mannich base acetate on the growth, cyclic AMP level, and Ca(2+) uptake by yeast cells in Saccharomyces cerevisiae]. / Vliianie antioksidanta atsetata osnovaniia Mannikha na rost, soderzhanie tsAMF i pogloshchenie Ca(2+) kletkami drozzhei Saccharomyces cerevisiae.

We have studied the effect of Mannich base acetate (MBA), an antioxidant, on growth of the yeast, as well as on the level of cAMP and Ca2+ uptake by them. We demonstrate that the antioxidant induced an increase in the level of cAMP in yeast cells and a decrease in Ca2+ uptake by the cells. The effect of the antioxidant depends on the level of Ca2+ in the growth medium.

Influence of (9Z)-12-hydroxy-9-dodecenoic acid and methyl jasmonate on plant protein phosphorylation.

The products of the lipoxygenase pathway, methyl jasmonic acid (MeJA) and (9Z)-12-hydroxy-9-dodecenoic acid (HDA), hardly changed the relative level of phosphorylated polypeptides (RLPPs) during 2 h of incubation: 15 and 17 kDa RLPPs were enhanced by HDA, but decreased by MeJA. RLPPs of 73 and 82 kDa were increased by both compounds. MeJA and HDA treatment induced specific and unspecific effects in some RLPPs. It was shown that HDA and MeJA increased protein kinase activity in the presence of 1 microM cAMP.

[The effect of pyrimidine derivatives on the sulfhydryl status of immunocompetent cells in vivo: the interrelationship with Ca2(+)-ATPase and antimutagenic activity]. / Vliianie pirimidinovykh proizvodnykh na sul'gidril'nyi status immunokompetentnykh kletok in vivo: vzaimosviaz' s Ca2(+)-ATFaznoi i antimutagennoi aktivnost'iu.

It was demonstrated in experiments on guinea pigs that a 30 mg/kg dose of ximedon changes the content of SH-groups in immunocompetent cells depending on the duration of drug administration and the organ to which the cells under test belong. Maximum increase of the SH-groups was recorded in the thymocytes. The dynamics of changes in Ca(2+)-ATPase activity repeated the fluctuations in the SH-group content in the splenic and bone marrow cells. In the bone marrow cells of CBA x C57B1 mice 3 mg/kg ximedon suppressed the induction of chromosome aberrations caused by cyclophosphane. The interrelationship of changes in the cell sulfhydryl status with Ca(2+)-ATPase activity and the antimutagenic effect of ximedon is discussed.

[Effect of pyrimidine derivatives on the regulation of active transport of calcium in the immunocompetent cells]. / Vliianie pirimidinovykh proizvodnykh na sistemu reguliatsii aktivnogo transporta kal'tsiia v immunokompetentnykh kletkakh.

Experiments with guinea pig immunocompetent cells and nonadherent and adherent mononuclear fractions of the peripheral blood of healthy donors, and patients with rheumatoid arthritis and purulent surgical infection disclosed different levels of Ca(2+)-ATPase activity. Thymectomy in adult guinea pigs led to diminution of activity of the enzyme in all lymphoid organs. The effect of ximedon (30 mg/kg) on Ca(2+)-ATPase activity depended on the thymus and duration of treatment with the drug, and was of a two-phase character.