[Comparative study of the safety, reactogenicity and antigenic activity of chemical and live brucellosis vaccines in a controlled epidemiological trial]. / Sravnitel'noe izuchenie bezvrednosti, reaktogennosti i antigennoi aktivnosti brutselleznoi khimicheskoi i zhivoi vaktsin v usloviiakh kontroliruemogo épidemiologicheskogo opyta.

The complex evaluation of the reactogenicity characteristics revealed that after immunization with chemical brucellosis vaccine systemic reactions observed in most of the vaccinees were mildly pronounced and local reactions, mildly and moderately pronounced, their duration not exceeding 48-72 hours. During 4 months after immunization the antigenic and immunogenic potency of chemical brucellosis vaccine was no different from that of live brucellosis vaccine; seropositive persons immunized with chemical and live brucellosis vaccines showed no statistically significant differences in the geometric mean of their antibody titers, as determined in a number of serological tests, for a year after immunization. The examination of the vaccinees 4 and 12 months after immunization revealed that the sensitizing activity of chemical brucellosis vaccine was, respectively, 12.2 and 2.5 times lower than the allergenic action of live brucellosis vaccine.

[Determination of the optimal dose of a cultured inactivated vaccine for preventing Japanese encephalitis]. / Opredelenie optimal'noi dozy kul'tural'noi inaktivirovannoi vaktsiny dlia profilaktiki iaponskogo éntsefalita.

The article presents the summarized results of the controlled field trial of a new inactivated liquid absorbed tissue-culture vaccine developed in the USSR. The optimal immunization dose has been determined: 1.5 ml of the vaccine introduced with a syringe or a jet injector. The intensity and character of postvaccinal immunity have been studied.

[Methodological principles of the determination of the information value of laboratory evaluation of the immunogenicity and reactivity of vaccines]. / O metodicheskikh printsipakh opredeleniia informativnosti laboratornykh testov otsenki immunogennosti i reaktogennosti vaktsin.

The correlation between the reactogenicity and immunogenicity characteristics of different smallpox vaccines, determined under the conditions of a strictly controlled field trial, has been studied by a number of laboratory tests. The most informative and reproducible laboratory methods for evaluating the provisory antigenic and immunogenic potency of smallpox vaccines are the evaluation of the character of lesions developing in rabbits as a result of the intradermal injection of the virus and the determination of the capacity of the virus for multiplication in the skin of a rabbit (reproduction). The most informative and reproducible method for evaluating the provisory reactogenicity of smallpox vaccines for humans is the determination of the capacity of the virus for multiplication at elevated temperature.

[Determination of the optimal inoculation dose of Brucella chemical vaccine]. / Opredelenie optimal'noi privivochnoi dozy brutselleznoi khimicheskoi vaktsiny.

Brucellosis chemical vaccine, when injected intramuscularly, proved to be low-reactogenic, postvaccinal reactions, both systemic and local, becoming less frequent and intense with the decrease of the dose from 2 to 0.5 mg. The highest antigenic and immunogenic activity was observed with a dose of 1 mg; out of 4 tested doses this dose produced the highest percentage of seroconversions, the highest titers of agglutinins and the longest time of their persistence in the subjects. When introduced in doses of up to 2 mg, brucellosis chemical vaccine did not induce pronounced sensitization. A high degree of correlation between the indices of seroconversion frequency in Wright's agglutination test and Huddleson's test was established after vaccination with all tested doses. This fact indicates that one of these tests can be used for the assessment of the antigenic and immunogenic activity of the vaccine at an early period after immunization (the term of observation not exceeding 3 months). On the basis of the complex comparison of the indices characterizing the reactogenicity and the antigenic and immunogenic activity of 4 tested doses of brucellosis chemical vaccine a dose of 1 mg was chosen as the optimum vaccination dose.

[Comparative study of the smallpox vaccines from B-51, EM-63 and L-IVP in a controlled epidemiological experiment. II. The characteristiics of the immunogenicity of the smallpox vaccines]. / Sravnitel'noe izuchenie ospennykh vaktsin iz shtammov B-51, EM-63 i L-IVP v usloviiakh kontroliruemogo épidemiologicheskogo opyta. Soobshchenie II. Kharakteristika immunogennosti ospennykh vaktsin.

Immunogenicity of smallpox vaccines prepared of EM-63, L-IVP, and B-51 strains was studied under conditions of strict controlled epidemiological trial. Skin reactions to revaccination and vaccines antigenic activity indices were detemined in the persons vaccinated. Changes in the virus-neutralizing and antibodies suppressing hemagglutination was the same in persons vaccinated with any of the preparations tested. The maximal virus-neutralizing antibodies level was determined 1 month after the vaccination and persisted without any essential changes for one year. The titre of hemagglutination inhibiting antibodies also reached the maximum in one month, but diminished gradually by the end of one year after the vaccination. There were found no significant differences in the antigenic activity of the vaccines. The vaccines studied also displayed no difference in the number and character of skin reactions to revaccination. In comparing the antibodies level and the character of skin reactions to revaccination it was found that the titres of hemagglutination inhibiting antibodies and virus-neutralizing antibodies of 1:40 and over were in the great majority of cases determined in the blood sera of the vaccinated persons with the immediate and negative reactions to revaccination, i. e. in those with intensive postvaccinal immunity.

[Comparative study of smallpox vaccines from strains B-51, EM-63 and L-IVP in a controlled epidemiologic trial. I. Reactogenic characteristics of the smallpox vaccines]. / Sravnitel'noe izuchenie ospennykh vaktsin iz shtammov B-51, EM-63, L-IVP v usloviiakh kontroliruemogo épidemiologicheskogo opyta. Soobshchenie I. Kharakteristika reaktogennosti ospennykh vaktsin.

A comparative study of smallpox vaccines prepared from the l-ivp zm-63 and b-51 strains was carried out under conditions of controlled epidemiological trial. Children subject to planned vaccination were scarified with commercial batches of the preparations under study (with the same infectious activity). As a result of investigations it was found that in the group of those scarified with the vaccine from zm-63 strain strong local and catarrhal reactions and some disturbances of general condition were more frequent than in those scarified with preparations from the l-ivp and b-51 strain. Along with this, the temperature elevation of various intensity showed no statistically significant difference in the groups a vaccinated with the vaccines compared. Integral analysis of signs characterising different manifestations of reactigenic properties of the vaccines compared led to the conclusion that preparations made of zm-6 strain had greater reactogenic properties.

[Relationship between the incidence of neurologic complications following smallpox vaccination and the generation and infectious activity of vaccine from strain L-IVP]. / Zavisimost' chastoty nevrologicheskikh oslozhnenii pri vaktsinatsii protiv ospy ot generatsii i infektsionnoi aktivnosti vaktsiny iz shtamma L-IVP.

A strictly controlled epidemiological trial was applied to the study of the incidence of neurological complications in children inoculated with vaccine from the L-IVP strain of the 2nd, 3rd, and 5th passages (generations). To ascertain a possible connection between the neuropathogenicity with the infectious activity the persons vaccinated were divided into 3 groups in accordance with the infectiousness level of the preparation obtained: the I group--3--5-10(8)OOU/ml; the II group--6--8-9-10(8) OOU/ml; the III group over 9-10(8) OOU/ml. The least number of neurological complications was recorded among children primarily inoculated with the vaccine of the 3rd passage with the infectiousness titre of over 9-10(8) OOU/ml.