Applications of Plant-Made Fibroblast Growth Factor for Human Pluripotent Stem Cells.

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) hold great potential in regenerative medicine. These cells can be expanded indefinitely in theory and are able to differentiate into different types of cells for cell therapies, drug screening, and basic biology studies. The reliable and effective propagation of hESCs and hiPSCs is important for their downstream applications. Basic fibroblast growth factor (bFGF) is critical to hESCs and hiPSCs for maintaining their pluripotency. Plant-produced growth factors are safe to use without potential contamination of infectious viruses and are less expensive to produce. In this study, we used rice cell-made basic fibroblast growth factor (RbFGF) to propagate hESCs and hiPSCs for at least eight passages. Both hESCs and hiPSCs cultured with RbFGF not only maintained the morphology but also the specific expression (OCT4, SSEA4, SOX2, and TRA-1-60) of PSCs, similar to those cultured with the commercial Escherichia coli-produced bFGF. Furthermore, both gene chip-based PluriTest and TaqMan hPSC Scorecard pluripotency analysis demonstrated the pluripotent expression profile of the hESCs cultured with RbFGF. In vitro trilineage assays further showed that these hESCs and hiPSCs cultured on RbFGF were capable of giving rise to cell derivatives of ectoderm, mesoderm, and endoderm, further demonstrating their pluripotency. Finally, chromosome stability was also maintained in hESCs cultured with RbFGF as demonstrated by normal karyotypes. This study suggests broad applications for plant-made growth factors in stem cell culture and regenerative medicine.

A modified approach to Industrial Pollution Projection System for the assessment of sectoral pollution loads in Bangladesh.

Industrial pollution in Bangladesh has posed a serious threat to human health, economic activity, and the environment. By emphasizing industries that produce major pollutants, substantial improvements can be made to pollution mitigation measures. In countries where primary pollution data is not readily available, the Industrial Pollution Projection System (IPPS) could be used to calculate the pollution load utilizing total industrial output or employment data. IPPS data, which was designed for developed countries like the USA, had been used directly for other countries without any normalization in previously reported studies. The main purpose of this study is to modify the current IPPS approach for any other country by incorporating specific correction factor for a specific country. In this study, a specific correction factor for Bangladesh was determined, taking into account the country's major polluting industries, and used to estimate the pollution scenario for the year 2020. The accuracy of the specific pollution intensities was also evaluated by comparing the data obtained using both gross output and employee number. According to this study, the top three air-polluting industries are structural clay products, cement-lime-plaster industry, and iron and steel industry. Similarly, for water pollution, the food industry, paper and paper product industry, and textile industry are the largest pollutant contributors. The detailed pollution load matrix in terms of air and water pollution is also developed, and can be used to predict both short-term and long-term scenarios of industrial pollution in Bangladesh, which eventually will assist the policy makers to adopt appropriate pollution management approach. Moreover, the methods developed in this study will help to tailor the IPPS data for any country and increase the accuracy of the pollution load.