ABSTRACT
Histone deacetylase inhibitors (HDACIs) are known to alter gene expression by both up- and down-regulation of protein-coding genes in normal and cancer cells. However, the exact regulatory mechanisms of action remain uncharacterized. Here we investigated genome wide dose-dependent epigenetic and transcriptome changes in response to HDACI largazole in a transformed and a non-transformed cell line. Exposure to low nanomolar largazole concentrations (Subject(s)
Depsipeptides/pharmacology
, Enhancer Elements, Genetic
, Histone Code/drug effects
, Histone Deacetylase Inhibitors/pharmacology
, Thiazoles/pharmacology
, Acetylation
, Cell Line
, Cell Line, Transformed
, Cytostatic Agents/pharmacology
, Dose-Response Relationship, Drug
, Enhancer Elements, Genetic/drug effects
, Genome
, Histone Deacetylases/physiology
, Histones/metabolism
, Oncogenes
, Promoter Regions, Genetic
, RNA Polymerase II/metabolism
, RNA, Messenger/metabolism
