ABSTRACT
Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.
Subject(s)
Monensin/biosynthesis , Propionates/pharmacology , Streptomyces/metabolism , Drug Resistance, Microbial , MutationABSTRACT
A diffusion plate method for the assay of the activity of lipases in large series of samples was worked out. When using this method the maximum deviation was found to be +/- 3.8%.
Subject(s)
Candida/enzymology , Lipase/analysis , Streptomyces/enzymology , Animals , Diffusion , Kinetics , Methods , Pancreas/enzymology , SwineABSTRACT
Daunomycinone, aglycone of the anthracycline antibiotic daunomycin, was transformed by a washed mycelia of Streptomyces aureofaciens B-96 in a buffer solution containing sucrose; the obtained product, dihydrodaunomycinone (9-(1-hydroxyethyl)-7,8,9,10-tetrahydro-6,7,9,11-tetrahydroxy-4-methoxy-5,12-naphthacenequinone), was identified by measuring basic physicochemical characteristics (IR, UV and visible spectra, mass spectra and NMR, optical rotation and m.p.).
