[Economic aspects in selection of treatment method for tibial and femoral shaft fractures]. / Ekonomiczny aspekt w wyborze metody leczenia zlaman trzonów kosci udowej i piszczeli.

The aim of the study was the analysis of the costs of the treatment the patients with tibial and femur shaft fractures treated operatively and conservatively. There were 643 adults and among them 145 patients with femur shaft fractures treated by intramedullary nailing using open Küntscher method (48 patients), closed Küntscher method (35 patients), plating (35 patients) and conservatively (19 patients). There were also 498 patients with tibial shaft fractures treated by intramedullary nailing using closed Küntscher method (105 patients), plating (109 patients), screws (82 patients) and conservatively (202 patients). The cost of the treatment was the lowest in groups of patients with femoral and tibial shaft fractures treated by intramedullary nailing using closed Küntscher method.

The activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in erythrocytes of rats with experimental neoplastic disease.

In erythrocytes of rats bearing Morris hepatoma 5123 the activities of superoxide dismutase, glutathione peroxidase and glutathione reductase as well as the level of reduced glutathione increased on the 10th day after transplantation of the tumor. In the second phase of the tumor growth (20 days after transplantation), the activities of glutathione peroxidase, glutathione reductase and the level of reduced glutathione in erythrocytes of the experimental animals were lower than in controls, whereas the activity of superoxide dismutase was at that time higher than in controls. On the other hand, the activity of catalase did not significantly differ from that found in healthy rats.

The content of N-acetylneuraminic acid in glycoproteins of erythrocyte membranes in Morris hepatoma 5123 bearing rats.

Changes in the content of N-acetylneuraminic acid in rat erythrocyte membranes at different stages of experimental tumour (Morris hepatoma 5123) development were examined. Its content was lowered on the 30th and 40th day after transplantation of the tumour cells, as compared to the results for normal healthy rats. As a result of the tumour growth, the content of N-acetylgalactosamine, galactose and mannose in rat erythrocyte membranes became lowered, whereas that of glucose remained unchanged. The content of fucose was raised at early stage of tumour growth, and remained at this high level till the 40th day of experiment.

The effect of an experimental neoplastic disease on the flux of sodium and potassium ions across red blood cells and on the lipid composition of their membranes.

The erythrocytes from Morris Hepatoma 5123 bearing rats took up Na+ and K+ ions from the incubation medium and released Na+ into the extracellular space at lower rates than did erythrocytes from intact control rats. The lipid composition of erythrocytes membranes from the tumor-bearing rats differed from that of membranes from unaffected rats, showing increased contents of phospholipid phosphorus and a decreased content of cholesterol, resulting in decreased cholesterol:phospholipid molar ratios.

[Modified surgical technique for treating hepatic echinococcosis]. / Modyfikacja metody operacyjnego leczenia bablowca watroby.

The authors present modified operational technique in hepatic echinococcosis. Echinococcus in 14 patients was removed by either peri-cystic excision or hepatic lobe excision with the closure of blood inflow into the liver by construction the blood vessels in hepato-duodenal ligament for 20 minutes. All patients survived. Biliary fistulas lasting for a few days up to 3 weeks were the only complications encountered. Presented modified operational technique of surgery is safe; it shortens the duration of surgery and prevents major intraoperative haemorrhages.

Polymorphonuclear neutrophils function in splenectomized patients.

Some essential functions of polymorphonuclear neutrophils (PMN) were evaluated in 30 patients splenectomized because of rupture of the spleen. These cells revealed normal random migration, adherence, unstimulated O2- and H2O2 production. Phagocytosis of viable staphylococci was higher than in controls, whereas chemotaxis, bactericidal capacity, aggregation and stimulated O2- and H2O2 production were significantly impaired. PMN from splenectomized patients manifested also the decreased intracellular myeloperoxidase activity. The percentage of cells with receptor for Fc IgG in peripheral blood was markedly decreased. Plasma of these patients induced increased adherence of autologous as well as control neutrophils. The possible mechanisms leading to the observed events are discussed.

[Glucosephosphate dehydrogenase activity and reduced glutathione level in the erythrocytes of children with lymphoblastic leukemia]. / Aktywnosc dehydrogenazy glukozo-6-fosforanowej i poziom zredukowanego glutationu w krwinkach czerwonych dzieci z ostra bialaczka limfoblastyczna.

The activity of glucose-6-phosphate dehydrogenase and the level of reduced glutathione were determined in the erythrocytes of children with acute lymphoblastic leukaemia treated by intensive combined chemotherapy (according to the authors from Memphis). The determinations were done before, during and after completion of treatment lasting about 3 years. A significant rise was observed in the activity of this enzyme and in the level of reduced glutathione in the groups of treated children and their fall to normal values after completion of treatment.

[Biochemical and morphological parameters of rats subjected to intensive physical effort]. / Wybrane parametry biochemiczne i morfologiczne u szczurów poddanych intensywnemu wisilkowi fizycznemu.

Lactate dehydrogenase activity was determined in the liver and skeletal muscle and types of muscle fibers were studied in m.gastrocnemius of male rats subjected to intensive physical exercise. In the skeletal muscle LDH4+5 isozymes activity and percent of white muscle fibers were increased. No changes in LDH activity and morphological parameters were found in the liver.

Glycogen resynthesis in the rat muscle and liver after physical exercise.

Glycogen resynthesis was studied in muscles and liver of Wistar rats after a single bout of physical exercise, which included 30 min of enforced swimming in water at 32 degrees C. The experiment was carried out on untrained rats which rested after the exercise from 30 min to 24 h, receiving food ad libitum. During the postexercise restitution a rise was observed in the glycogen content of both tissues. This rise was more rapid in the muscles than in the liver. During the first 9 hours of rest the muscle glycogen content reached 92.6% of the initial value, and after 24 hours it exceeded this value by 20%. On the other hand, the resynthesis of hepatic glycogen was 67% of the initial value after 9 hours, and after 24 hours the liver glycogen content reached only 76.5% of this value. It has been pointed out also that the restitution of glycogen in the muscles occurs in two steps of the intensive increase, while in the liver it is more steady.

[Glutathione reductase activity in erythrocytes of rats with transplantable Morris 5123 hepatoma]. / Aktywnosc reduktazy glutationowej w krwinkach czerwonych szczurów z przeszczepialnym watrobiakiem Morrisa 5123.

The activity of NADPH- and NADH-dependent erythrocyte glutathione reductase was determined in rats with Morris 5123 hepatoma at different stages of tumor development (10, 20, 30 and 40 days after transplantation). During the early stage of tumor growth the activity of glutathione reductase with either of these coenzymes was increased. In the late stage of the disease the activity of NADPH-dependent glutathione reductase fell below control values. The obtained results are discussed in the light of previous observations on the effects of this neoplasm on the metabolism of erythrocytes.

[Changes in the activity of glucose-6-phosphate dehydrogenase and in the content of reduced glutathione in red blood corpuscles in the case of tumorous diseases in the maxillofacial region (author's transl)]. / Die Aktivitätsveränderungen der Glukose-6-phosphat-dehydrogenase und des Gehalts an reduziertem Glutathion der Erythrozyten bei Tumorerkrankungen im Kiefer-Gesichtsbereich.

The activity of glucose-6-phosphate dehydrogenase and the glutathione content of red blood corpuscles were determined in twenty-five patients with tumors (of which nineteen were malignant and six, nonmalignant) as well as in a control group of twelve normal subjects. The activity of glucose-6-phosphate dehydrogenase was markedly higher in all of the tumor patients than it was in the controls. Also, the activity of glucose-6-phosphate dehydrogenase was found to increase with the time of existence of an untreated tumor.--Increased glutathione contents were determined especially in the early stages of tumor development. In patients with a relatively long record of malignant and nonmalignant tumorous diseases as well as in normal subjects there was observed no or only an inconsiderable increase in the content of glutathione.--This method of examination is assuming considerable importance for the differential diagnosis of tumors located in the maxillofacial region.

Effect of exercise on glycogen level in muscles and liver in rats.

The effect of exercise of glycogen level in skeletal muscles and liver was studied in Wistar rats. The previously untrained animals were subjected to one-time exercise in form of swimming in water at 32 degrees C for 10, 20 and 30 min. The glycogen level in the muscles (in g per 100 g of tissue) fell down during the first 10 minutes of the exercise by a mean value of 0.45 g. During the following 10 minutes the decrease was smaller amounting on the average to 0.1 g. After 30 min the glycogen level in the muscles was about 0.1 g/100 g of tissue. Respective falls of glycogen level in the liver were on the average 0.99 g and 0.40 g/100 g of tissue. After 30 min of exercise the glycogen level in the liver was 1.2 g/100 g of tissue. The fall of glycogen level in the muscles was similar at all times during exercise in all animals, but in the liver fairly significant differences were observed in the first 10 min between individual groups of rats. Later on during exercise the differences in the liver glycogen falls decreased.