ABSTRACT
AIM: Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burneli persistence in dynamics of infectious process in patients with Q fever. MATERIALS AND METHODS: 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time. PCR (RT-PCR) (marker - groEL gene fragment). RESULTS: Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). CONCLUSION: This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.
Subject(s)
Bacterial Proteins/genetics , Chaperonin 60/genetics , Coxiella burnetii/genetics , Q Fever , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Real-Time Polymerase Chain Reaction , Adult , Female , Humans , Male , Middle Aged , Q Fever/diagnosis , Q Fever/geneticsABSTRACT
The article deals with the results of study of 35 patients with Q-fever and 30 donors to determine the content of rosette-forming neutrophils, lysozyme activity of blood and saliva. On the second week of disease it was revealed the activation of compensative immune mechanisms and on third and fourth week inhibition of these mechanisms.
Subject(s)
Muramidase/blood , Neutrophils/metabolism , Q Fever/blood , Saliva/metabolism , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Muramidase/immunology , Neutrophils/immunology , Q Fever/immunology , Saliva/immunologyABSTRACT
AIM: To ascertain regularities of development of cellular immunity in patients with Astrakhan fever (AF) with reference to AF severity, treatment and course. MATERIAL AND METHODS: Cellular factors of immunity--relative and absolute contents of T-lymphocytes (early and late), T-helpers and T-suppressors, B-lymphocytes, neutrophils (early and late)--were studied in 75 patients with AF (63 patients with moderate and 12 patients with severe disease) in the course of the disease progression (weeks 1-5). The patients received antibacterial treatment--doxicyclin, rifampicin and doxicycline in combination with alpha 2-realdiron and gamma-reaferon. RESULTS: Examination of immunocompetent cells has revealed suppression of T- and B-immunity for 2 weeks in moderate AF and 3 weeks in severe AF. The number of T-helpers was low for 3 weeks while of T-suppressors rose beginning from week 2. Immunoregulatory index Tx/Tc remains low till the end of the disease. The number of early and late neutrophils increased since weeks 2 of the disease in moderate and week 3 in severe AF. CONCLUSION: Doxicycline efficiency was higher than that of rifampicin. Interferon preparations decrease relative number of early and late T-lymphocytes, neutrocytes and T-helpers but their absolute number is at the control level. Relative content of T-suppressors and B-lymphocytes is as in controls but their absolute content is higher.
Subject(s)
B-Lymphocytes/immunology , Boutonneuse Fever/immunology , T-Lymphocytes/immunology , Adolescent , Adult , Aged , Boutonneuse Fever/drug therapy , Doxycycline/therapeutic use , Humans , Interferon Type I/therapeutic use , Interferon alpha-2 , Interferon-alpha , Middle Aged , Recombinant Proteins , Rifampin/therapeutic useABSTRACT
In 65 patients with bacteriologically confirmed gastrointestinal salmonellosis of moderate severity (41 males and 24 females) the disease in an acute phase was characterized by activation of complement system and specific antibody formation, by a growth in the levels of IgA, G and M. In early convalescence IgA and IgM remained high, IgG in females returned to normal, in males was still high. An inverse correlation was found between functional activity of complement components C3, C4 and C5 and levels of IgM, IgG.