Outbreak of ranavirus infection in sheatfish, Silurus glanis (L.), in Poland.

Ranavirus was detected in adult sheatfish, with clinical signs, on a Polish fish farm in February. Farm isolates induced a strong cytopathic effect in vitro and were identified by electron microscopy and PCR amplification of the ranavirus specific gene fragment. Restriction analysis with the Acc I enzyme showed that isolated ranaviruses were different from the epizootic heamatopoietic necrosis virus (EHNV). We sequenced a fragment of the major capsid protein (MCP) gene and found that isolates were similar to other strains of ranaviruses, available in GenBank.

[EFFECTIVE SCREENING METHODS FOR CARBOHYDRATE METABOLISM DISORDERS].

There has been a significant increase in diabetes morbidity in the past years. Prolonged asymptomatic progression of disease presents a problem and leads to late diagnosis and development of complications. Present study shows efficiency of screening measures to determine actual spread of carbohydrate metabolism disorders among residents of Odessa, frequency of type 2 diabetes mellitus risk factors determination and prevention of complications.

[Treatment of experimental broncho-pleural fistula with myoplasty]. / Leczenie doswiadczalnej przetoki oskrzelowo-oplucnowej metoda myoplastyki.

Surgical experiment of closing broncho-pleural fistula by muscle flap from the chest wall was conducted on the sheep model. The procedure was completed in two stages. During the first thoracotomy the middle lobe of the right lung was removed. In the second stage the vascularised muscle flap of the latissimus dorsi was inserted in the pleural space and stitched onto the bronchi stump closing broncho-pleural fistula. Microscopic investigation of lung, bronchus and muscle tissue performed after each step of procedure showed the inflammatory process primarily and the regression of it after the second stage. Well vascularised muscle flap is the best tissue for repair of broncho-pleural fistula.

[Morphologic and toxicologic evaluation of response to subcutaneous para-osseous implants Medpol-1 and Medpol-2 in rats]. / Ocena morfologiczna i toksykologiczna wplywu podskórnych przykostnych wszczepów Medpol-1 i Medpol-2 na organizm szczura.

Cobalt alloys produced in Poland and meant for orthopedic implants were morphologically and toxicologically evaluated. They were implanted subcutaneously in the para-osseous region of rats' heads. The results of the experimental tests strongly suggest the advisability of a clinical research project (study) on the examined cobalt alloys: Medpol-1 and Medpol-2.

The influence of inhibitors of cellular synthesis and UV irradiation on interferon induction by RNA from Piptoporus betulinus.

The effect of cycloheximide, chloramphenicol, ethidium bromide (EB), aurin tricarboxylic acid (ATA), and actinomycin D on the production of interferon (IFN) in human embryo fibroblasts (HAT) and L929 cells, stimulated with RNA from Piptoporus betulinus (Pb-RNA) was studied. Treatment of HAT cells with cycloheximide superinduced the production of IFN. Subsequent treatment with cycloheximide and actinomycin D chloramphenicol and actinomycin D or ATA and actinomycin D early after induction also enhanced the production of interferon, but the highest titers of interferon were observed when HAT cells were superinduced with cycloheximide and actinomycin D 11 hours after induction. In L929 cells the superinduction was efficient shortly after induction, but the IFN titers obtained were still very low. The effect of UV irradiation of HAT cells was also examined, but no enhancement of IFN production after induction with Pb-RNA was observed. Pb-RNA produced IFN in the spleen and liver of Swiss mice but no enhancement of IFN production was observed after treatment of mice with cycloheximide, ATA and EB.

The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the induction of interferon in human embryo fibroblasts.

The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the production of human interferon was studied in cultures of human embryo fibroblasts (HAT). Interferon (IFN) was induced with Hertfordshire strain of Newcastle Disease Virus (NDV-H) partially inactivated with UV irradiation (NDV-Huv). The interfering properties of various NDV-Huv doses were studied in HAT tissue cultures. The highest IFN yield (IFN U per 10(6) cells) was obtained by using 100 TCID50 NDV-Huv/cell and incubating the induced cultures for 24 h at 37 degrees C. Irradiation of HAT tissue culture with UV doses of 400 and 700 erg/mm2 did not increase IFN production as expected. However, incubation of HAT tissue culture with nontoxic doses of L-ascorbic acid 10(-3) and 10(-4) M or with nontoxic doses of 1 and 4 mM calcium chloride caused an increased IFN production by over 200%.

Studies of RNA isolated from Piptoporus betulinus as interferon inducer.

Crude RNA isolated from Piptoporus betulinus and two fractions of it obtained after the chromatography contain single-stranded RNA. The crude RNA, but not its chromatographic fractions, are resistant to the pancreatic RNA-ase. The crude preparation of RNA from P. betulinus contains beside ribose, considerable amounts of glucose, galactose and mannose. The crude RNA from P. betulinus induces in the human fibroblasts interferon with specific activity of 400 units per mg of protein.