[The influence of an air ionizer on the air ionic composition].

The air ionic composition in a classroom was determined during the operation of a Neo Tec XJ-2100 ionizer (Germany). The amount of ions in the air was measured before and after the switch-on of the ionizer. It was shown to vary depending on the mode of operation of the device and differ from the recommended normal values. The necessity of checking up the work of air ionizers both in living quarters and at workplaces is discussed with the application of air ion counters making it possible to carry out monitoring of the air ionic composition and estimate its compliance with the sanitary and hygienic norms.

[Antiherpetic effect of RNA-tilorone molecular complex in cell culture].

The authors studied the antiviral effect of an interferonogenic yeast RNA-tilorone molecular complex (MC) compared to the Virolex, videly used antiherpetic drug, and standard interferon (IFN) alpha/beta inducer poly(I)poly(C) in Vero cells culture infected with herpes simplex type I virus (HSV-1). The tilorone contained by MC has been shown to be twice less toxic and twice more active against HSV than its free molecules. The value of chemotherapeutic index (CI) of Virolex in experiments with Vero cells reaches 2500, CI poly(I)poly(C) and MC being 324; the last value meets the requirements for promising drugs.

[Interferon inducers--from theory to practice]. / Induktory interferonu--vid teoriï do praktyky.

The authors present up-to-date information concerning different inducers of different interferon types in vivo and in vitro. Interactions between the structure and interferon-inducing activity of high and low molecular weight inducers are in the focus of this paper. There are also a lot of data, concerning different biological properties of interferon-inducing compounds. Different aspects of inducers use as antiviral and immunomodulating drugs are also discussed.

[Effect of interferonogenic molecular complex of yeast RNA--tilorone on DNA, RNA and protein synthesis in vitro]. / Vplyv interferonohennoho molekuliarnoho kompleksu drizhdzhova RNK--tyloron na syntez DNK, RNK ta bilka in vitro.

In the experiments in vitro using the primary mononuclear cells (MNC) culture of the human peripheral blood the influence of interferonogenic yeast RNA-tilorone molecular complex on the DNA, RNA and protein synthesis was studied. The complex was shown to inhibit the insertion of 3H-thymidine, 3H-uridine and 3H-leucine into DNA, RNA and protein of MNC total pool (by 13, 1 and 40% respectively); that was practically conformed with this synthesis inhibition upon to a natural origin polynucleotide interferon inducers--lariphan (9, 0 and 57% respectively) and ridostin (9, 0 and 56% respectively) action, and at the same time rather less than poly(I)-poly(C) (14, 5 and 62% respectively). In the case of preliminary cell stimulation by the mitogen PHA the complex revealed comitogenic action at a concentration 25 micrograms/ml, that corresponded to optimal for interferonogenesis; the increase of the doses till 100-1000 micrograms/ml lead to in the reversal effect. To proceed from mutual relation between interferonogen preparations influence on the mentioned synthesis and their cytotoxicity the conclusion was about made the complex promising usage as an interferon inducer both in vitro and in vivo conditions.

[Thermal denaturation of molecular complexes formed by single stranded RNA with tilorone]. / Temperaturna denaturatsiia molekuliarnykh kompleksiv odnolantsiugovoï RNK tyloronom.

The study of thermal denaturation for molecular complexes formed during interaction between phage MS2 single-stranded RNA and tilorone has been carried out. Both certain increase of Tm (by on 1.1) and the narrowing of complexes melting interval, delta T, (by 5.6 degrees C), have been detected. The value of hyperchromic effect, H, increases twice. The conclusion is made concerning the formation of double-stranded regions in single-stranded RNA molecules in the process of RNA--tilorone interactions. These results are discussed as an additional proof of our previous presumption concerning stabilization of spontaneously forming double-stranded RNA regions by tilorone binding.

[Spectrophotometric study of the interaction of single-stranded RNA with tilorone]. / Spektrofotometrychne vyvchennia vzaiemodiï odnolantsiuhovoï RNK z tyloronom.

The spectrophotometric study of complex formation between phage MS2 single-stranded RNA and tilorone has been carried out. It was found that with components concentration forming "strong linkage" the absorption spectra of RNA-tilorone complex in UV-region experience hyperchromic and bathochromic shifts; besides, a new absorption maximum appears at 280 nm. The changes of spectral properties have been also detected in the long-waved region of the complex spectra. The conclusion has been made that in the course of single-stranded RNA with tilorone interactions a complex formation takes place due to interacalation between spontaneously appeared two-stranded regions in the structure of a single-stranded RNA and tilorone; which component stabilizes these structures and assures there by the previously described interferonogenic activity of a single-stranded RNA--tilorone complexes.

[The depression of the reproduction of the human immunodeficiency virus by the use of Bogomolets antireticular cytotoxic serum]. / Pryhnichennia reproduktsiï virusu imunodefitsytu liudyny za dopomohoiu antyretykuliarnoï tsytotoksychnoï syrovatky Bohomol'tsia.

The action of the Bogomolets' antireticular cytotoxic serum (ACS) as a HIV-1 reproduction inhibitor was studied on the model of human lymphoblastoid cell line MT-4/BRU (acute infection). The HIV-1 reproduction inhibition was estimated according to cell protection level for virus cytopathic action and p24 antigen level in the cultural media. The both tests data on ACS usage in dilutions 1:160-1:640 prove that the virus inhibition effect which may be compared with anti-HIV action of the well-known agent, azidothymidine, was observed. At this dilutions range ACS is devoid of the cell toxicity. The conclusion about perspectivity of ACS usage in the AIDS complex therapy was drawn.