[AEROMONAS BACTERIA ISOLATED FROM BITHYNIIDAE MOLLUSKS AND THEIR HABITATS: SPECIES COMPOSITION AND BIOLOGICAL PROPERTIES. COMMUNICATION 1].

The purpose of this investigation was to study the species composition and biological properties of Aeromonas bacteria isolated from Bithyniidae mollusks and their habitat (a water reservoir). The Bithyniidae mollusks and water from their habitat were the material to be studied. A total of 176 Aeromonas strains were isolated from the mollusks and water. A. veronii, A. hydrophila, and A. ichthiosmia were most common in the mollusks and A. veronii and A. ichthiosmia were in the water. All the strains isolated had hemolytic activity and no lysozyme or plasma coagulase activity. The magnitude of lecithinase and antilysozymic activities and biofilm formation of the Aeromonas bacteria varied with the isolation source of their strains.

[THE STRUCTURE AND SOME BIOLOGICAL PROPERTIES OF GRAM-NEGATIVE BACTERIA CONSTITUTING THE MICROSYMBIOCENOSIS OF PROSOBRANCHIA MOLLUSKS (BITHYNIIDAE). COMMUNICATION 2].

The goal of this investigation was to study the structure and biological properties (antilysozymic, activity and biofilm formation) of gram-negative bacteria isolated from Bithyniidae mollusks and their habitat (water reservoir waters and soil). A total of 160 gram-negative bacterial strains isolated from the mollusks of the Bithyniidae family and their habitat were the material to be, studied. Psedomonas, Comamonas, and Acinetobacter held the lead in the structure of microbiocenosis of Bithyniidae mollusks, the first intermediate host of Opisthorchis filineus, while Acinetobacter did in the habitat. The antilysozymic activity of the water strains was shown to be an order of magnitude higher than that of the strains isolated from the mollusks.

[BIOLOGICAL PROPERTIES OF BACTERIA OF THE FAMILY ENTEROBACTERIACEAE AS COMPONENTS OF MICROSYMBIOCENOSIS OF THE FIRST INTERMEDIATE HOSTS OF O.FELINEUS].

The objective of the investigation was to study the biological properties (antilysozyme activity (ALA), biofilm formation (BFF), and virulence factors) of different Enterobacteriaceae species isolated from Bithyniidae mollusks and their habitats. A total of 117 strains isolated from Bithyniidae mollusks of the genera Codiella and Bithynia and those from their habitats were the material to be studied. Thus, comparison of the mean values of ALA in Enterobacteriaceae species suggests that the strains isolated from the mollusks and their aqueous habitat did not virtually differ in this indicator. Also, there were no statistically significant differences in the detection rate of the Enterobacteriaceae strains having a pronounced antilysozyme activity and in that of mollusks circulating in the aqueous habitat when compared with the strains isolated from the mollusks. Comparison of BFF in the aqueous bacterial strains and mollusk microbiota representatives revealed the highest values in the former; just lower value was noted in the latter. Soil Enterobacteriaceae isolates had very low BFF values.

[RATIONAL ASPECTS OF BACTERIOPHAGES USE].

AIM: Analysis of existing aspects of bacteriophage use and study features of their lytic activity by using various techniques. MATERIALS AND METHODS: Effect of monophages and associated bacteriophages (staphylococci, piopolyvalent and piocombined, intestiphage, pneumonia klebsiella and polyvalent klebsiella produced by "Microgen") was studied with 380 strains of Staphylococcus aureus and 279 cultures of Klebsiella pneumoniae in liquid and solid nutrient media. From patients with intestinal disorder, sensitivity was analyzed to 184 strains of Salmonella genus bacteria 18 serological variants to salmonella bacteriophages, 137 strains of Escherichia coli (lactose-negative, hemolytic), as well as some members of OKA groups (21 serovars) to coli-proteic and piopolyvalent bacteriophages. Lytic ability of the piobacteriophage against Klebsiella and Proteus genus bacteria was determined. RESULTS: Staphylococcus aureus was sensitive to staphylococcus bacteriophage in 71.6% of cases and to piobacteriophage--in 86.15% of cases. A 100% lytic ability of salmonella bacteriophage against Salmonella spp. was established. Sensitivity of E. coli of various serogroups to coli-proteic and piobacteriophage was 66 - 100%. Klebsiella, Proteus genus bacteria were sensitive to piobacteriophage in only 35% and 43.15% of cases, respectively. CONCLUSION: A more rational use of bacteriophages is necessary: development of a technique, evaluation of sensitivity of bacteria to bacteriophage, introduction of corrections into their production (expansion of bacteriophage spectra, determination and indication of their concentration in accompanying documents).

[Microsymbiocenosis of Codiella mollusks as a basis for symbiotic relations in the parasite-host system in opisthorchiasis].

The purpose of the first step of microparasitocenosis investigation was to study the microbiocenosis of the first intermediate hosts of O. felineus--prosobranch gastropods of the genus Codiella, as well as their habitats. Materials were collected in the Iryum River of the Ob-Irtysh basin. The microflora of mollusks, water, and soil from their habitats was examined. The predominant flora was Aeromonas species in the biocenosis of mollusks and Enterobacteriaceae in the microbiocenosis of the water basin and soil. Examination of the microbial communities in the mollusks and their habitats showed that the range of microbial populations of mollusks was wider in species composition as compared to the microbiocenosis of soil and water.