ABSTRACT
The study included 15 patients with purulent inflammatory diseases of maxillofacial area and 25 patients with facial bone necrosis induced by synthetic drugs. Pro- and anti-inflammatory cytokines levels in saliva and wound fluid were analyzed in two groups. The results proved cytokines to play important role in jaw necrosis induced by drugs containing red phosphorus.
Subject(s)
Cytokines/immunology , Illicit Drugs/adverse effects , Jaw Diseases/chemically induced , Morphine Derivatives/adverse effects , Osteonecrosis/chemically induced , Adult , Cytokines/analysis , Female , Humans , Interleukin-10/analysis , Interleukin-10/immunology , Jaw Diseases/immunology , Male , Middle Aged , Morphine Derivatives/administration & dosage , Osteonecrosis/immunology , Saliva/chemistry , Saliva/immunology , Transforming Growth Factor beta/analysis , Transforming Growth Factor beta/immunology , Tumor Necrosis Factor-alpha/analysis , Tumor Necrosis Factor-alpha/immunology , Young AdultABSTRACT
Molecular study of congenital immune factors was conducted in 45 patients with inflammatory diseases of maxillofacial area. The study focused on expression of cationic low-molecular peptides α- and Β-defensines in oral mucosa. These peptides are involved in antibacterial activity and regulation of immune reactions. The results showed 20-fold increase in defensines expression in oral mucosa of patients with inflammatory diseases of maxillofacial area when compared to control group (12 healthy individuals) thus proving an important role they play in the development of the disease. Therapy with cytokines may be indicated in these cases.
Subject(s)
Cellulitis/immunology , Maxilla , Mouth Mucosa/immunology , alpha-Defensins/immunology , beta-Defensins/immunology , Cellulitis/surgery , Cellulitis/therapy , Cytokines/immunology , Cytokines/therapeutic use , Face , Gene Expression , Humans , alpha-Defensins/analysis , alpha-Defensins/genetics , beta-Defensins/analysis , beta-Defensins/geneticsABSTRACT
Levels of expression of hBD-1 gene (beta-defensin 1) and Toll-like receptors (TLR1, TLR2, TLR6) in cells of cervical mucosa in healthy nonpregnant and healthy pregnant women as well as in pregnant women with urogenital infection was measured by developed RT-PCR systems. During normal pregnancy compared with nonpregnant women, increase of TLRs genes expression which was correlated with increase of hBD-1 gene expression was observed. During urogenital infection in pregnant women compared with healthy pregnant, 10- fold and 50-fold increase of TLR1 and TLR2 genes expression respectively was associated with 2.5-fold decrease of hBD-1 gene expression in cervical mucosa. In group of women with untrauterine infection more marked increase of TLRs genes expression was observed. Thus significant changes (TLRs, antimicrobial peptides, cytokines etc.) in cells of cervical mucosa can be used as prognostic criteria for development of intrauterine infection.
