[Fibrinogen determination by the Clauss method. Reagents and analyzers].

The Clauss method was recommended by the WHO to measure plasma fibrinogen levels. The purpose of this study was to assess the application of test systems made by the RENAM Research-and-Production Association (Moscow) on different types of clinical laboratory analyzers to measure fibrinogen by the Clauss method. The calibration line is shown to be of major value in obtaining correct results on each coagulometer. The findings suggest the accuracy and reproducibility of the determination of fibrinogen concentrations by the Clauss when the REHAM test systems are used to measure fibrinogen concentrations on various analyzers if accurate calibration lines are obtained.

[Standardization of coagulologic technique for antithrombin III estimation and its clinical application].

A standard assay procedure for estimating antithrombin III activity was derived from the Abildgaard technique as follows: 1) to decrease errors at the preanalytical stage, by using preliminarily defibrinated lyophilized plasma-calibrator and 2) to increase measurement accuracy, by using the concentration of a fibrinogen preparation and simplifying the analytical procedure. The estimation of plasma antithrombin activity in patients with pulmonary thromboembolism or sepsis points to the changes in antithrombin III activity.

[Stable thrombin preparations for the Klauss method of determination of thrombin time and fibrinogen level]. / Stabil'nye preparaty trombina dlia opredeleniia trombinovogo vremeni i kontsentratsii fibrinogena po Klaussu.

A method was elaborated enabling an isolation of a stable and highly active thrombin to be used in determining the thrombin time and fibrinogen concentration in plasma. The stability of the thus obtained preparations was studied, which showed no changes in the activity of their liquid variations for as long as 28 days at 2-8 degrees C.