ABSTRACT
The present study demonstrates the siderophore production of two strains of Pseudomonas fluorescens and two of Ps. chlororaphis. The antimicrobial activities of these strains were studied against Salmonella typhimurium, Staphylococcus aureus, Fusarium culmorum, F. oxysporum and Aspergillus niger. Despite equal siderophore activities with various Pseudomonas spp. as measured by the chrome azurol S assay, the study shows how siderophore activity does not correlate with the antibacterial activity against food pathogens or with the antimould activity against pathogenic moulds. Furthermore, the results illustrate how siderophores are able to act both as growth inhibitors and stimulators.
Subject(s)
Bacteria/drug effects , Fungi/drug effects , Pseudomonas/metabolism , Siderophores/biosynthesis , Anti-Bacterial Agents , Anti-Infective Agents/metabolism , Anti-Infective Agents/pharmacology , Foodborne Diseases/microbiology , Foodborne Diseases/prevention & control , Humans , Pseudomonas/growth & development , Siderophores/pharmacology , Species SpecificityABSTRACT
Boneless loins from both sides of 20 pig carcasses were divided into five sections each and assigned equally to five packaging treatments: 100 % CO(2); 50 % CO(2)/50 % N(2); 25 % CO(2)/ 75 % N(2); 25 % CO(2)/65 % N(2)/10 % O(2) and vacuum. Loin sections were packaged in bags of low O(2) permeability, then stored in darkness at 1 °C for up to 22 days. Retail chops were cut from the sections and displayed in oxygen-permeable film under light at 3 °C for 3 additional days. The O(2) concentrations in packages with nominally anoxic atmospheres were 0.1-0.4 %. Sections stored in 25 % CO(2)/65 % N(2)/10 % O(2) had more surface greying and greening, stronger off-odour and psychrotropic counts after storage were more than one log(10) higher compared to sections from the other four treatments. Displayed chops from sections stored in 25 % CO(2)/65 % N(2)/10 % O(2) also had greying/greening at an outer layer of the chops. Off-odour of chops was most pronounced for treatments with 10 % O(2) and vacuum. Drip loss from loin sections was highest for those in 100 % CO(2) (4.2 %) and lowest for those in vacuum (3.2 %). In conclusion, storage in CO(2) or CO (2)N (2) atmospheres benefitted the overall shelf life of pork.
ABSTRACT
A microbiological screening method for the detection of irradiation of frozen poultry meat was developed on the basis of the combined use of total cell count by the direct epifluorescent filter technique (DEFT) and viable cell count by the aerobic plate count method (APC). Samples of ground, deboned poultry leg were irradiated or not with dose levels of 3, 5 and 7 kGy using an electron beam accelerator. All samples were frozen before the irradiation treatment. The average values of the differences between DEFT and APC counts in control samples and those irradiated with doses of 3, 5 and 7 kGy were 1.14 log units for control samples, and 3.16, 3.68 and 3.79 log units for the irradiated samples. A difference of at least 2 log units can therefore be considered as a limit value indicating probable irradiation treatment necessitating further investigations.
