ABSTRACT
Preparations of low-molecular-weight DNA were obtained from gonads of various hydrobiont species by alcohol precipitation technique. An enzymatic hydrolysis-based method for producing soluble low-molecular-weight DNA was developed. All the nucleic acid components of the preparations obtained were separated by electrophoresis, and their molecular weights were determined. It was demonstrated that high activities of endogenous enzymes (nucleases and proteases) in milts of hydrobionts correlated with the degree of hydrolysis of their nucleic acids.
Subject(s)
DNA/metabolism , Deoxyribonucleases/metabolism , Endopeptidases/metabolism , Fishes/metabolism , Gonads/metabolism , Mollusca/metabolism , Oligonucleotides/metabolism , Alcohols , Animals , Chemical Precipitation , DNA/chemistry , DNA/isolation & purification , Electrophoresis , Gonads/chemistry , Hydrolysis , Male , Molecular Weight , Oligonucleotides/chemistry , Oligonucleotides/isolation & purification , Pancreatin/metabolismABSTRACT
The study of the immunotropic action of DNA from salmon milt showed that it increased the antiinfectious resistance of mice to Escherichia coli and Salmonella enteritidis, stimulated the antibody response to the thymus-dependent corpuscular antigen (sheep erythrocytes), increased the production of the antibody forming cells (AFC) in the murine spleen and intensified the absorptive and digestive activities of the cells of the mononuclear phagocytes. The immunotropic properties of the DNA permitted to broaden the DNA application spectrum with its supplementing by immunodeficiency of various genesis and diseases with phagocytic protection mechanisms. It is quite possible that the salmon milt DNA be used as a food additive.
Subject(s)
DNA/immunology , Salmonidae/immunology , Spermatozoa/immunology , Animals , Antibody-Producing Cells/drug effects , Antibody-Producing Cells/immunology , DNA/administration & dosage , DNA/isolation & purification , Escherichia coli Infections/immunology , Guinea Pigs , Hypersensitivity, Delayed/immunology , Immunity, Cellular/drug effects , Immunity, Innate/drug effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Molecular Weight , Phagocytosis/drug effects , Time FactorsABSTRACT
It has been developed a receipt of a new treatment-and-prophylactic products based on fish farce with treatment-and-prophylactic additive--DNA. It is recommended to be produced. As biological active additive keeps its initial pharmacological characteristics under technological processing this product is recommended for rendering general effect and increasing of physical and mental activity of organism.
