ABSTRACT
Specificity of regulation of genes expression at the transcriptional and posttranscriptional levels is typical for spermatogenesis in Drosophila and mammals, including humans. It becomes apparent in the existence of testis specific NXF (nuclear export factor). We have shown that the Dm NXF1 (SBR) protein is present in considerable amounts at all stages of the spermatogenesis. Using the antibody for the C-terminal part of the Dm NXF1 protein we have shown the cytoplasmic localization of the Dm NXF1 protein at early stages of the spermatogenesis. This protein is localized in the nuclear envelope at the stage of rounded spermatid. During the period of elongation, the Dm NXF1 protein has a polar localization, and is located only along one side of the extended spermatid nucleus. At the stage of spermatid individualization, this protein in the form of large cytoplasmic granules moves to the tail of the spermatozoon.
Subject(s)
Drosophila Proteins/physiology , Drosophila melanogaster/physiology , Nuclear Proteins/physiology , RNA-Binding Proteins/physiology , Spermatocytes/metabolism , Spermatogenesis , Animals , Cytoplasm/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Immunohistochemistry , Male , Nuclear Envelope/metabolism , Nuclear Proteins/metabolism , RNA, Messenger/metabolism , RNA-Binding Proteins/metabolismSubject(s)
Multiple Myeloma/pathology , Plasma Cells/pathology , Adult , Aged , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Autoradiography , Cell Division/drug effects , Combined Modality Therapy , Cytophotometry , Female , Humans , Male , Middle Aged , Multiple Myeloma/drug therapy , Multiple Myeloma/mortality , Plasma Cells/drug effects , Prognosis , Recurrence , Remission InductionSubject(s)
Anemia, Aplastic/blood , Bone Marrow Diseases/blood , Hematopoiesis , Preleukemia/blood , Adolescent , Adult , Anemia, Aplastic/diagnosis , Bone Marrow Diseases/diagnosis , Bone Marrow Diseases/etiology , Bone Marrow Examination , Chronic Disease , Diagnosis, Differential , Female , Hepatitis/blood , Hepatitis/complications , Hepatitis/diagnosis , Humans , Leukocyte Count , Male , Middle Aged , Preleukemia/diagnosisABSTRACT
Feulgen-DNA cytophotometry and 3H-thymidine autoradiography were used to evaluate therapeutic cytapheresis effects on cell cycle distributions of peripheral blood leukemic cells in 15 acute leukemia, 5 CML and 8 CLL patients and of bone marrow erythroblasts in 18 PV patients. Both individual cytapheresis procedures and a long-term program of cytapheresis altered cell cycle distributions and biochemistry which may be summarized as follows: An increase in percentage of cells in S-phase (acute leukemia, CML); a decrease in the differences between values of S-cell number obtained by cytophotometry and autoradiography (acute leukemia, CML, PV); a decrease in some cases in enlarged fraction of cells in G2 (blast crisis of CML, PV); an activation of G0----G1 transition and an increase in the PAS-positive lymphocyte fraction in CLL patients. An apparent increase of blasts in S-phase was found after a pheresis in acute leukemia patients who responded to subsequent chemotherapy. In "nonresponders", a pheresis had no effect on cell cycle distributions. The results of the study demonstrate that despite the wide variability of individual responses, cytapheresis activates cell proliferation and metabolic processes in patients with hematological malignancies.
Subject(s)
Bone Marrow/pathology , Cell Cycle , Leukapheresis , Leukemia/blood , Polycythemia Vera/pathology , Blast Crisis , Erythroblasts/pathology , Polycythemia Vera/blood , Time FactorsSubject(s)
Multiple Myeloma/pathology , Plasma Cells/pathology , Adult , Aged , Autoradiography , Cell Division , Female , Humans , Male , Middle AgedSubject(s)
Leukemia, Lymphoid/pathology , Adolescent , Adult , Bone Marrow/pathology , Humans , Leukemia, Lymphoid/immunology , Lymphocytes/immunology , MaleABSTRACT
Electrophoretic mobility of murine lymphocytes of the spleen was investigated during the development of reaction to damage and to the initial stages of induced cancerogenesis. These two processes differ in kinetics data of the change of electrophoretic mobility. A correlation was found between the electrophoretic mobility and ability of the cell for blast transformation under the influence of lectin PHA.
