ABSTRACT
AIM: To evaluate the efficacy of the combined use of citicoline (neipilept) and levodopa/carbidopa (nakom) in the rotenone model of Parkinson's disease in rats. MATERIAL AND METHODS: Rotenone was administrated during 14 days in dose 2 mg/kg/day subcutaneously. The duration of treatment was 7 days, intragastrically. Alteration of locomotor behavior components, muscular rigidity in resistance to passive flexion in the ankle joint and signs of extrapyramidal disorders were assessed. RESULTS AND CONCLUSION: Combined therapy led to the decrease in muscle rigidity (the decrease of gibbosity in resistance to passive flexion in the ankle joint). There was a decrease in oligokinesia that emerged in higher vertical and horizontal locomotor activity of experimental animals, decrease in the time of head turning during climbing down the pole and total time of climbing down the pole. The combination of drugs had a more pronounced therapeutic effect on extrapyramidal disorders compared to monotherapy.
Subject(s)
Antiparkinson Agents/therapeutic use , Carbidopa/therapeutic use , Cytidine Diphosphate Choline/therapeutic use , Levodopa/therapeutic use , Nootropic Agents/therapeutic use , Parkinson Disease/drug therapy , Animals , Disease Models, Animal , Drug Combinations , Drug Therapy, Combination , Male , Rats , Rats, WistarABSTRACT
This work was aimed at comparative analysis of the two experimental models of chronic arthritis induced in rats by the administration of (i) complete Freund's adjuvant (CFA) and (ii) carrageenan. The clinical and histological signs of pathology were assessed using a categorical rating system. It is established that the administration of CFA leads to the development of pathology closely resembling rheumatoid arthritis. In contrast, the administration of c.arrageenan promotes chronic inflammatory arthritis without autoimmune component. The results can serve a basis for selecting a methodological approach to assess the effectiveness of drugs with anti-rheumatoid, anti-inflammatory, and/or immunomodulatory properties.
Subject(s)
Arthritis, Experimental , Animals , Arthritis, Experimental/chemically induced , Arthritis, Experimental/metabolism , Arthritis, Experimental/pathology , Arthritis, Experimental/physiopathology , Female , Rats , Rats, WistarABSTRACT
The aim of this work was to study the behavioral and histopathomorphological signs of peripheral neuropathy development in male Wistar rats on the model of alcoholic neuropathy. Chronic consumption of ethanol solution with concentration increasing from 7.47 to 26.2% (w/w) resulted in neuropathy (allodynia) de- velopment after 8 weeks of chronic alcohol administration. The behavioral signs of allodynia became significant on the 8th week and were retained up to the end of experiment (15 weeks of ethanol administration). The reference drug gabapentin effectively reduced the manifestation of allodinia. Histological exami- nation of sciatic nerve preparations from animals killed after ethanol consumption for 5, 10 and 15 weeks revealed the development of histopathomorphological pattern with increasing duration of chronic alcoholization. At the initial stage, the morphological basis of observed behavioral manifestations was provided by excess lipid deposition in peri/epineurium of nerve specimens). The further increase in treatment duration (up to 10 and 15 weeks) was associated with demye- lination and development of inflammation of the sciatic nerve. This experimental model allows one to investigate the efficacy of new neuroprotective and ana- lgesic substances - potential drugs for both prevention and management of neuropathy.
Subject(s)
Alcoholic Neuropathy/pathology , Behavior, Animal/drug effects , Demyelinating Diseases/etiology , Disease Models, Animal , Hyperalgesia/psychology , Sciatic Nerve/drug effects , Alcoholic Neuropathy/prevention & control , Alcoholic Neuropathy/psychology , Amines/therapeutic use , Animals , Cyclohexanecarboxylic Acids/therapeutic use , Demyelinating Diseases/pathology , Demyelinating Diseases/prevention & control , Dose-Response Relationship, Drug , Ethanol/administration & dosage , Gabapentin , Hyperalgesia/prevention & control , Sciatic Nerve/pathology , Time Factors , gamma-Aminobutyric Acid/therapeutic useABSTRACT
Research has suggested that catalase plays a role in mediating ethanol's psychopharmacological effects. Catalase is an enzyme that oxidizes ethanol to acetaldehyde. It has been reported that when catalase activity is reduced by 3-amino-1,2,4-triazole (AT), rats reduce their intake and preference for ethanol. The present study assessed the effects of AT on the brain amino acids levels following ethanol administration in Wistar rats. The study consisted of three parts. In the first part, we found no effects of acute and chronic intraperitoneally administered acetaldehyde on amino acids dialysate levels in nucleus accumbens. In the second part, AT was administered five hours prior to ethanol or its vehicle. Ethanol significantly affected the levels of taurine in rat pre-treated with AT. In the final part, ethanol was administered following the pre-treatment with AT but the dependent variable was the concentration of ethanol in the brain.
Subject(s)
Acetaldehyde/pharmacology , Brain/metabolism , Ethanol/pharmacology , Taurine/metabolism , Amitrole/pharmacology , Animals , Brain/drug effects , Catalase/antagonists & inhibitors , Catalase/metabolism , Drug Interactions , Enzyme Inhibitors/pharmacology , Glutamic Acid/drug effects , Glutamic Acid/metabolism , Male , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , Rats , Rats, Wistar , Time FactorsABSTRACT
Intraperitoneal administration of marinobufagenin resulted in a reliable and dose-dependent suppression of ethanol self-administration in drug- and experimentally naive DBA/2 mice. The findings suggest that Na/K-ATPase contributes to both mediation of the ethanol reinforcing properties and the mood regulation.
Subject(s)
Alcohol Drinking , Bufanolides/pharmacology , Conditioning, Operant/drug effects , Enzyme Inhibitors/pharmacology , Reinforcement, Psychology , Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors , Alcohol Drinking/psychology , Animals , Male , Mice , Mice, Inbred DBAABSTRACT
We studied the analgesic effect of olipiphate, a product of lignin, against writhing provoked by intraperitoneal injection of acetic acid. Paracetamol was used as the reference drug. Both agents dose-dependently decreased the number of motor reactions caused by the irritant. Olipiphate possessed analgesic activity and efficiency comparable with those of paracetamol, but produced a more long-lasting effect.
