ABSTRACT
Gas-liquid chromatography was used to study the composition of fatty acids and hydrocarbons of different types of Mycobacteria, including M. tuberculosis, opportunistic Mycobacteria and acid-fast saprophytes. In terms of composition of fatty acids and hydrocarbons, clinical and laboratory strains of M. tuberculosis are very similar. The cellular higher fatty acids of M. tuberculosis differ much from those of opportunistic Mycobacteria and acid-fast saprophytes. The findings can be used for the identification and differentiation of different types of Mycobacteria.
Subject(s)
Bronchoalveolar Lavage Fluid/microbiology , Carbohydrate Metabolism , Fatty Acids/metabolism , Mycobacterium tuberculosis/classification , Sputum/microbiology , Tuberculosis, Pulmonary/microbiology , Carbohydrates/chemistry , Chromatography, Gas , Fatty Acids/chemistry , Humans , In Vitro Techniques , Mycobacterium tuberculosis/metabolismABSTRACT
The role of intracellular protein fractions, isolated from M. tuberculosis virulent strains in the process of the electrophoretic separation, in the development of delayed hypersensitivity reactions has been studied. Low reactogenicity of protein fractions has been established on the basis of the development of faint immunopathological reactions in sensitized animals. High-molecular fraction 1 may be used as a sensitive preparation for the differentiation of individual mycobacterial species.
Subject(s)
Bacterial Proteins/immunology , Hypersensitivity, Delayed/etiology , Mycobacterium/immunology , Animals , Bacterial Proteins/isolation & purification , Cell Adhesion/immunology , Diagnosis, Differential , Guinea Pigs , Hypersensitivity, Delayed/diagnosis , Hypersensitivity, Delayed/pathology , Leukocytes/immunology , Lung/pathology , Mycobacterium Infections/diagnosis , Mycobacterium avium/immunology , Mycobacterium phlei/immunology , Mycobacterium tuberculosis/immunology , Nontuberculous Mycobacteria/immunology , Tuberculin TestSubject(s)
Bacterial Proteins/analysis , Mycobacterium tuberculosis/classification , Densitometry , Electrophoresis, Polyacrylamide Gel , Mycobacterium bovis/analysis , Mycobacterium bovis/classification , Mycobacterium phlei/analysis , Mycobacterium phlei/classification , Mycobacterium tuberculosis/analysis , Species SpecificitySubject(s)
Glomerulonephritis/microbiology , L Forms , Mutation , Animals , Chronic Disease , Humans , L Forms/isolation & purification , MiceABSTRACT
Bacteriological analysis of urine of 150 patients with chronic pyelonephritis was performed. As a result mycoplasma was isolated from urine of 25 patients. Mycoplasma and Coli bacteria or Proteus were isolated simultaneously from urine of 10 patients. Biochemical properties and sensitivity to antibiotics of 9 isolates were studied. The data provided recommendation of the urine analysis for the presence of mycoplasma.
Subject(s)
Mycoplasma/isolation & purification , Pyelonephritis/microbiology , Urine/microbiology , Anti-Bacterial Agents/pharmacology , Chronic Disease , Culture Media , Humans , Microbial Sensitivity Tests , Mycoplasma/metabolism , Penicillin Resistance , Pyelonephritis/urine , Time FactorsABSTRACT
The authors present the data concerning the study of 200 patients suffering from chronic pyelonephritis; in 28 of these L-forms of bacteria were revealed in the urine. Of 46 L-cultures isolated from these patients 13 reversed into bacterial forms, 8 failed to reverse and were referred to the stable L-forms; the rest 25 L-cultures perished during the 8th--10th passage. This led to a supposition that the relapses and exacerbations of the infectious process in pyelonephritis were associated with the change of the L-forms into bacterial ones, and that the persistence of the L-forms in the kidney tissue promoted the maintenance of the chronic process.
