ABSTRACT
A simple, rapid, precise and economical high performance thin layer chromatographic method has been developed and validated for determination of rosiglitazone in its tablet dosage form using caffeine as an internal standard. It was performed on silica gel 60 GF(254) thin layer chromatographic plates as a stationary phase using mobile phase methanol:toluene:chloroform:triethylamine (1:8:0.5:0.5 v/v/v/v) and the detection was carried out in the absorbance mode at 264 nm showing R(f) value 0.31 for rosiglitazone and 0.52 for caffeine. The linear regression data curve shows good linear relationship in the concentration range 1.0-7.0 µg/µl. The content uniformity test was carried out as per USP specification of the content uniformity test of 85-115%. The percent drug estimated of rosiglitazone from two different marketed formulations were found to be in the range 99.83-100.21. The recovery of drugs was carried out by standard addition method were found to be 100.21±1.06 and 100.04±0.30 by height and area respectively. The method was validated with the determination of accuracy, precision, specificity, linearity detector response and ruggedness. The proposed method provides a faster and cost effective quality control tool for routine analysis of content uniformity test for rosiglitazone in tablet formulation.
ABSTRACT
Two simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of lansoprazole and domperidone in pharmaceutical dosage form. Method A involves formation of Q-absorbance equation at 256.0 nm (isoabsorptive point) and at 294.2 nm while method B is two wavelength method where 277.6 nm, 302.1 nm were selected as lambda(1) and lambda(2) for determination of lansoprazole and 231.3 nm, 292.0 nm were selected as lambda(1) and lambda(2) for determination of domperidone. Both the methods were validated statistically and recovery studies were carried out. The Beer's law limits for each drug individually and in mixture was within the concentration range of 5-50 mug/ml. Linearity of lansoprazole and domperidone were in the range of 24-36 mug/ml and 8-12 mug/ml, respectively. The proposed methods have been applied successfully to the analysis of the cited drugs either in pure form or in pharmaceutical formulations with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
ABSTRACT
Fixed dose combination tablets containing ambroxol HCl and cetirizine HCl are clinically used as mucolytic and antiallergic. Several spectrophotometric and HPLC methods have been reported for simultaneous estimation of these drugs with other drugs. The drugs individually and in mixture obeys Beer's law over conc. range 1.2-4.4 microg/mL for cetirizine HCL and for ambroxol HCL 15-52 microg/mL at all five sampling wavelengths (correlation coeff. well above 0.995). The mean recoveries from tablet by standard addition method were 100.18% (+/-2.4) and 100.66 % (+/-2.31). The present work reports simple, accurate and precise spectrophotometric methods for their simultaneous estimation from tablet dosage form.
Subject(s)
Ambroxol/analysis , Anti-Allergic Agents/analysis , Cetirizine/analysis , Expectorants/analysis , Drug Combinations , Spectrophotometry, Ultraviolet , TabletsSubject(s)
Analgesics, Non-Narcotic/chemistry , Analgesics, Non-Narcotic/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/pharmacology , Benzimidazoles/chemistry , Benzimidazoles/pharmacology , Central Nervous System Depressants/chemistry , Central Nervous System Depressants/pharmacology , Analgesics, Non-Narcotic/toxicity , Animals , Antifungal Agents/toxicity , Benzimidazoles/toxicity , Central Nervous System Depressants/toxicity , Chemical Phenomena , Chemistry, Physical , Female , Fungi/drug effects , Lethal Dose 50 , Male , Mice , Microbial Sensitivity Tests , Pain Measurement/drug effects , Structure-Activity RelationshipABSTRACT
Two sensitive methods for the determination of phenothiazine derivatives based on the formation of coloured salts of these drugs formed with Brilliant Blue or Orange-II in the presence of 0.1 M HCl are described. The ion pair salts formed are quantitatively extracted into chloroform and the dried chloroform solutions analyzed using spectrophotometry.
