ABSTRACT
Hepatic energy stores are essential to liver viability. We used a mouse liver perfusion model and MR spectroscopy to study the effect of adding two precursors of ATP (adenine and ribose) on ATP dynamics during ischemia and reperfusion. Using Krebs-Henseleit buffer with or without added adenine and ribose made little difference in the ATP decay rate during ischemia, but the recovery of ATP during reperfusion was more complete when adenine and ribose were added to the buffer. These findings suggest that the addition of the precursors of ATP, adenine and ribose, to perfusate after ischemia can accelerate and enhance ATP recovery.
Subject(s)
Adenosine Triphosphate/analysis , Ischemia , Liver/blood supply , Magnetic Resonance Spectroscopy , Adenine/pharmacology , Animals , Liver/analysis , Mice , Perfusion , Ribose/pharmacologyABSTRACT
A total of 14 patients with advanced visceral carcinoma which produced carcinoembryonic antigen (CEA) were treated in a Phase I study with IV infusion of affinity purified baboon anti-CEA antibody (dosage range from 0.1 to 2 mg/kg body weight). In all 14 patients, the antibody infusion caused a decrease (26% to 97%) in the plasma level of CEA. The degree of decrease depended upon the patients initial CEA level and the amount of antibody given. In 12 of the 14 patients "free" circulating antibody was observed. The plasma half-life of the antibody ranged from 0.7 to 21 h and the duration of detectable free antibody ranged from 3 to 216 h postinfusion. No toxicity was observed for the dosage range of antibody tested. In 9 of the 14 patients a low titer anti-baboon antibody response occurred between 14 and 28 days postinfusion. No clinical regression of carcinoma was documented. In 7 of the 14 patients disease remained stable during the 28-day study period. By the end of the 28-day study period plasma CEA levels had returned to at least the preinfusion level in 11 of the 14 patients.
Subject(s)
Adenocarcinoma/therapy , Antibodies, Neoplasm/therapeutic use , Carcinoembryonic Antigen/immunology , Adenocarcinoma/immunology , Adult , Aged , Animals , Antibodies, Anti-Idiotypic/analysis , Carcinoembryonic Antigen/analysis , Drug Evaluation , Female , Humans , Male , Middle Aged , Neoplasm Metastasis , Papio/immunologyABSTRACT
The CT scans of a woman with left flank and lower abdominal pain were interpreted as showing a mass adjacent to the tail of the pancreas. Imaging with Tc-99m sulfur colloid and emission computerized tomography revealed that the CT scan abnormality represented functioning splenic tissue and thereby avoided unnecessary surgical exploration.
