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Chem Commun (Camb)
; 52(45): 7284-7, 2016 Jun 07.
Article
in English
| MEDLINE
| ID: mdl-27181840
ABSTRACT
Site-specific RNA modification with methyl cyclopropene moieties is performed by T7 in vitro transcription. An existing unnatural base is functionalized with a cyclopropene moiety and used in transcription reactions to produce site-specifically cyclopropene-modified RNA molecules. The posttranscriptional inverse electron demand Diels-Alder cycloaddition reaction with a selected tetrazine-fluorophore conjugate is demonstrated.