Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 11 de 11
Filter
Add more filters










Publication year range
1.
Photodiagnosis Photodyn Ther ; 41: 103194, 2023 Mar.
Article in English | MEDLINE | ID: mdl-36402375

ABSTRACT

BACKGROUND: Antimicrobial photodymanic therapy mediated by methylene blue has been investigated as an adjunctive to periodontal treatment but the dimerization of photosensitizer molecules reduces the phototoxic effects. Sodium dodecyl sulfate is a surfactant that may control this aggregation. The aim of this study was evaluated the photodynamic effect of methylene blue in sodium dodecyl sulfate in periodontitis. METHODS: 36 participants with periodontitis were selected and allocated randomly in two group for intervention and other two for control - all of them were treated with scaling and root planing before aPDT. Three periodontal evaluations were done: at the selection time, at the day of intervention and thirty-day after this. Pre-irradiation time was 1 min and 2 min for irradiation. Laser (Therapy XT, DMC, São Carlos, Brazil) with wavelength of 660 nm and 100 mW of power was used. Two photosensitizer solutions with 100 µM methylene blue was used, one of them was in water and other in 0,25% of sodium dodecyl sulfate. Two sites of each participant were selected for the experimental procedures. Microbiological evaluations were performed to quantify microorganisms before and immediately after intervention. Quantitative microbiological evaluation was the primary outcome; morphological aspects of bacterial colony, and clinical probing depth was the secondary one. RESULTS: There was no significant difference between the groups in both bacterial reduction and the clinical parameter evaluated. CONCLUSION: The effect of methylene blue in surfactant did not cause enough phototoxic effects that could promote reduction of periodontal pocket depth.


Subject(s)
Anti-Infective Agents , Chronic Periodontitis , Periodontitis , Photochemotherapy , Humans , Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Methylene Blue/pharmacology , Methylene Blue/therapeutic use , Surface-Active Agents , Combined Modality Therapy , Sodium Dodecyl Sulfate/therapeutic use , Periodontitis/drug therapy , Anti-Infective Agents/therapeutic use , Adjuvants, Immunologic/therapeutic use , Dental Scaling , Root Planing/methods , Chronic Periodontitis/drug therapy
2.
Photodiagnosis Photodyn Ther ; 27: 132-136, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31152876

ABSTRACT

BACKGROUND: Antimicrobial photodynamic therapy (aPDT) has been investigated as an adjunctive to periodontal treatment but the dosimetry parameters adopted have discrepancies and represent a challenge to measure efficacy. There is a need to understand the clinical parameters required to obtain antimicrobial effects by using aPDT in periodontal pockets. The aim of this study was to investigate parameters relating to the antimicrobial effects of photodynamic therapy in periodontal pockets. MATERIAL AND METHODS: This randomized controlled clinical trial included 30 patients with chronic periodontitis. Three incisors from each patient were selected and randomized for the experimental procedures. Microbiological evaluations were performed to quantify microorganisms before and after treatments and spectroscopy was used to identify methylene blue in the pocket. A laser source with emission of radiation at wavelength of ʎ = 660 nm and output radiant power of 100 mW was used for 1, 3 and 5 min. One hundred µM methylene blue was used in aqueous solution and on surfactant vehicle. RESULTS: The results demonstrated the absence of any antimicrobial effect with aqueous methylene blue-mediated PDT. On the other hand, methylene blue in the surfactant vehicle produced microbial reduction in the group irradiated for 5 min (p < 0.05). Spectroscopy showed that surfactant vehicle decreased the dimer peak signal at 610 nm. CONCLUSION: Within the parameters used in this study, PDT mediated by methylene blue in a surfactant vehicle reached significant microbial reduction levels with 5 min of irradiation. The clinical use of PDT may be limited by factors that reduce the antimicrobial effect. Forms of irradiation and stability of the photosensitizers play an important role in clinical aPDT.


Subject(s)
Methylene Blue/therapeutic use , Periodontal Pocket/drug therapy , Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Surface-Active Agents/chemistry , Combined Modality Therapy , Drug Compounding , Female , Humans , Male , Methylene Blue/administration & dosage , Periodontal Index , Photosensitizing Agents/administration & dosage , Time Factors
3.
Photodiagnosis Photodyn Ther ; 23: 32-34, 2018 Sep.
Article in English | MEDLINE | ID: mdl-29807147

ABSTRACT

Antimicrobial photodynamic therapy (aPDT) has been used to treat periodontal disease, thus the aim of this study was to investigate red light (ʎ = 660 nm) attenuation in gingival tissue. This clinical trial included 30 patients with chronic periodontitis; three incisors from each patient were selected for the experimental procedures. A laser source with a radiant power output of 100 mW was used. Two digital photographs were taken of each selected incisor (in frontal and occlusal position). The images were analyzed in the ImageJ program. The results demonstrated that at a 3 mm distance from the laser probe, there is an attenuation of light intensity of 50%, along frontal and occlusal views. Light attenuation in gingival tissue should be considered when setting optimal parameters for antimicrobial photodynamic therapy or photobiomodulation.


Subject(s)
Gingiva/metabolism , Infrared Rays , Light , Periodontitis/physiopathology , Chronic Disease , Humans , Lasers, Semiconductor
4.
Photodiagnosis Photodyn Ther ; 17: 173-179, 2017 Mar.
Article in English | MEDLINE | ID: mdl-27993650

ABSTRACT

Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters, contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy that presents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitizing compound (PS) and light to generate oxidative stress that has demonstrated effective antimicrobial activity against a broad-spectrum of pathogens, including C. albicans. This fungus senses glucose inducing an upregulation of membrane transporters that can facilitate PS uptake into the cell. The aim of this study was to evaluate the effects of glucose on methylene blue (MB) uptake and its influence on PDI efficiency when combined to a red LED with central wavelength at λ=660nm. C. albicans biofilms were grown on hydrogel disks. Prior to PDI assays, MB uptake tests were performed with and without glucose-sensitization. In this system, the optimum PS administration was determined as 500µM of MB in contact with the biofilm during 30min before irradiation. Irradiation was performed during 3, 6, 9, 12, 15 and 18min with irradiance of 127.3mW/cm2. Our results showed that glucose was able to increase MB uptake in C. albicans cells. In addition, PDI without glucose showed a higher viability reduction until 6min; after 9min, glucose group demonstrated a significant decrease in cell viability when compared to glucose-free group. Taken together, our data suggest that glucose is capable to enhance MB uptake and modulate photodynamic inactivation of C. albicans biofilm.


Subject(s)
Biofilms/drug effects , Candida albicans/drug effects , Glucose/pharmacology , Methylene Blue/pharmacokinetics , Photochemotherapy/methods , Photosensitizing Agents/pharmacokinetics , Bacteriological Techniques , Methylene Blue/pharmacology , Photosensitizing Agents/pharmacology
5.
Photodiagnosis Photodyn Ther ; 15: 25-7, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27179711

ABSTRACT

Antimicrobial photodynamic therapy (APDI) has been used to treat localized infection and the aim of this study was to evaluate the effect of APDI combined with fluconazole in suspension of Candida albicans. C. albicans ATCC90028 was subcultured onto Sabouraud agar and inocula were prepared at yeast density of 1×10(6)CFU/mL. Methylene blue (MB) was used with concentration of 100mM. Yeast cells were incubated for 30min in 24-well plate and then irradiated by LED (660nm; 690mW; A=2.7cm(2); I=250mW/cm(2)) with radiant exposure of 30, 60, and 120J/cm(2). The same APDI setup was used with 2h fluconazole (0.5µg/mL) incubation. A UV-vis optical absorption spectroscopy was achieved following fractionated irradiation up to 960s. There were substantial differences in the killing effect following MB-mediated APDI and C. albicans was eradicated in the both APDI groups. The fluconazole combined to APDI delayed the complete inactivation of the yeast (p<0.05). Spectroscopy showed a decrease in absorption following irradiation for all absorption peaks. APDI presented an antagonist effect in the presence of fluconazole.


Subject(s)
Candida albicans/drug effects , Candida albicans/radiation effects , Cell Survival/drug effects , Cell Survival/radiation effects , Fluconazole/administration & dosage , Photochemotherapy/methods , Antifungal Agents/administration & dosage , Candida albicans/physiology , Cell Survival/physiology , Combined Modality Therapy/methods , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Drug Therapy, Combination/methods , Light , Photosensitizing Agents/administration & dosage
6.
Exp Biol Med (Maywood) ; 241(1): 40-5, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26202374

ABSTRACT

In this study, we looked at the possible effects of low-level laser therapy (LLLT) on blood flow velocity, and serotonin (5-HT) and cholinesterase levels in patients with chronic headache associated with temporomandibular disorders (TMD). LLLT has been clinically applied over the past years with positive results in analgesia and without the report of any side effects. The understanding of biological mechanisms of action may improve clinical results and facilitate its indication. Ten patients presenting headache associated with TMD completed the study. An 830-nm infrared diode laser with power of 100 mW, exposure time of 34 s, and energy of 3.4 J was applied on the tender points of masseter and temporal muscle. Blood flow velocity was determined via ultrasound Doppler velocimetry before and after laser irradiation. The whole blood 5-HT and cholinesterase levels were evaluated three days before, immediately, and three days after laser irradiation. Pain score after treatment decreased to a score of 5.8 corresponding to 64% of pain reduction (P < 0.05). LLLT promoted a decrease in the blood flow velocity (P < 0.05). In addition, the 5-HT levels were significantly increased three days after LLLT (P < 0.05). The cholinesterase levels remained unchanged at the analyzed time points (P > 0.05). Our findings indicated that LLLT regulates blood flow in the temporal artery after irradiation and might control 5-HT levels in patients suffering with tension-type headache associated to TMD contributing to pain relief.


Subject(s)
Blood Flow Velocity/radiation effects , Headache/therapy , Phototherapy , Regional Blood Flow/radiation effects , Serotonin/blood , Temporomandibular Joint Disorders/complications , Adult , Cholinesterases/blood , Female , Headache/physiopathology , Humans , Middle Aged , Pain/physiopathology , Severity of Illness Index , Treatment Outcome , Young Adult
7.
Photodiagnosis Photodyn Ther ; 12(1): 131-5, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25461964

ABSTRACT

BACKGROUND: The purpose of this study was to evaluate the antibacterial effects of photodynamic action of methylene blue (MB) against Aggregatibacter actinomycetemcomitans organized on biofilm. METHODS: After the biofilm growth in 96 flat-bottom well plate, the following groups were used: control group, untreated by either laser or photosensitizer (PS); MB group or dark toxicity group, which was exposed to MB alone (100µM) for 1min (pre-irradiation time); laser group, irradiated with laser for 5min in the absence of PS and three antimicrobial photodynamic inactivation (APDI) groups, with three exposure times of 1, 3 and 5min of irradiation, corresponding to fluences of 15, 45, and 75J/cm(2) respectively. The results were compared to the control group for statistical proposes. Scanning electronic microscope analysis was used to access structural changes in biofilm. RESULTS: Red laser alone and MB alone were not able to inactivate bacterial biofilm. APDI groups showed differences when compared to the control group and they were dependent on the exposure time. No statistically significant differences were observed among the APDI groups at 1 and 3min of irradiation. On the other hand, 5min of APDI showed 99.85% of bacterial reduction (p=0.0004). In addition, the biofilm loose its structure following 5min APDI. CONCLUSIONS: The results of this study suggest that A. actinomycetemcomitans biofilm can be inactivated by MB mediated APDI.


Subject(s)
Aggregatibacter actinomycetemcomitans/drug effects , Aggregatibacter actinomycetemcomitans/physiology , Biofilms/drug effects , Biofilms/growth & development , Methylene Blue/administration & dosage , Photochemotherapy/methods , Apoptosis/drug effects , Apoptosis/radiation effects , Biofilms/radiation effects , Cell Survival/drug effects , Cell Survival/radiation effects , Dose-Response Relationship, Radiation , Light , Photosensitizing Agents/therapeutic use
8.
Photochem Photobiol Sci ; 13(3): 595-602, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24496397

ABSTRACT

Antimicrobial photodynamic therapy (APDT) may become a useful clinical tool to treat microbial infections, and methylene blue (MB) is a well-known photosensitizer constantly employed in APDT studies, and although MB presents good efficiency in antimicrobial studies, some of the MB photochemical characteristics still have to be evaluated in terms of APDT. This work aimed to evaluate the role of MB solvent's ionic strength regarding dimerization, photochemistry, and photodynamic antimicrobial efficiency. Microbiological survival fraction assays on Escherichia coli were employed to verify the solution's influence on MB antimicrobial activity. MB was evaluated in deionized water and 0.9% saline solution through optical absorption spectroscopy; the solutions were also analysed via dissolved oxygen availability and reactive oxygen species (ROS) production. Our results show that bacterial reduction was increased in deionized water. Also we demonstrated that saline solution presents less oxygen availability than water, the dimer/monomer ratio for MB in saline is smaller than in water and MB presented a higher production of ROS in water than in 0.9% saline. Together, our results indicate the importance of the ionic strength in the photodynamic effectiveness and point out that this variable must be taken into account to design antimicrobial studies and to evaluate similar studies that might present conflicting results.


Subject(s)
Anti-Infective Agents/pharmacology , Methylene Blue/pharmacology , Photochemotherapy/methods , Sodium Chloride/chemistry , Water/chemistry , Bacterial Load/drug effects , Bacterial Load/radiation effects , Escherichia coli/drug effects , Escherichia coli/radiation effects , Osmolar Concentration , Oxygen/chemistry , Photochemical Processes , Photosensitizing Agents/pharmacology , Reactive Oxygen Species/chemistry , Solvents/chemistry , Spectrum Analysis
9.
Antimicrob Agents Chemother ; 57(1): 445-51, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23129051

ABSTRACT

The objective of this study was to evaluate whether Candida albicans exhibits altered pathogenicity characteristics following sublethal antimicrobial photodynamic inactivation (APDI) and if such alterations are maintained in the daughter cells. C. albicans was exposed to sublethal APDI by using methylene blue (MB) as a photosensitizer (0.05 mM) combined with a GaAlAs diode laser (λ 660 nm, 75 mW/cm(2), 9 to 27 J/cm(2)). In vitro, we evaluated APDI effects on C. albicans growth, germ tube formation, sensitivity to oxidative and osmotic stress, cell wall integrity, and fluconazole susceptibility. In vivo, we evaluated C. albicans pathogenicity with a mouse model of systemic infection. Animal survival was evaluated daily. Sublethal MB-mediated APDI reduced the growth rate and the ability of C. albicans to form germ tubes compared to untreated cells (P < 0.05). Survival of mice systemically infected with C. albicans pretreated with APDI was significantly increased compared to mice infected with untreated yeast (P < 0.05). APDI increased C. albicans sensitivity to sodium dodecyl sulfate, caffeine, and hydrogen peroxide. The MIC for fluconazole for C. albicans was also reduced following sublethal MB-mediated APDI. However, none of those pathogenic parameters was altered in daughter cells of C. albicans submitted to APDI. These data suggest that APDI may inhibit virulence factors and reduce in vivo pathogenicity of C. albicans. The absence of alterations in daughter cells indicates that APDI effects are transitory. The MIC reduction for fluconazole following APDI suggests that this antifungal could be combined with APDI to treat C. albicans infections.


Subject(s)
Candida albicans/drug effects , Candidiasis/drug therapy , Methylene Blue/pharmacology , Photosensitizing Agents/pharmacology , Virulence Factors/antagonists & inhibitors , Animals , Antifungal Agents/pharmacology , Candida albicans/growth & development , Candida albicans/pathogenicity , Candida albicans/radiation effects , Candidiasis/microbiology , Candidiasis/mortality , Cell Wall/drug effects , Cell Wall/radiation effects , Female , Fluconazole/pharmacology , Inheritance Patterns , Lasers, Semiconductor , Light , Male , Mice , Mice, Inbred BALB C , Osmotic Pressure , Oxidative Stress , Survival Analysis , Virulence Factors/metabolism
10.
Photomed Laser Surg ; 28(6): 835-9, 2010 Dec.
Article in English | MEDLINE | ID: mdl-21142725

ABSTRACT

OBJECTIVE: The aim of this study was to investigate the effect of low-level laser therapy (LLLT) on the treatment of burning mouth syndrome (BMS). In addition, the laser effect was compared on the different affected oral sites. MATERIALS AND METHODS: Eleven subjects with a total of 25 sites (tongue, lower lip, upper lip, and palate) affected by a burning sensation were selected. The affected areas were irradiated once a week for three consecutive weeks with an infrared laser (λ = 790 nm). The probe was kept in contact with the tissue, and the mucosal surface was scanned during the irradiation. The exposure time was calculated based on the fluence of 6 J/cm(2), the output power of 120 mW, and the area to be treated. Burning intensity was recorded through a visual analog scale before and after the treatment and at the 6-week follow-up. The percentage of the improvement in symptoms was also obtained. RESULTS: Burning intensity at the end of the laser therapy was statistically lower than at the beginning (p < 0.01). Patients reported an 80.4% reduction in the intensity of symptoms after laser treatment. There was no statistical difference between the end of the treatment and the 6-week follow-up, except for the tongue site. CONCLUSION: Under the investigated parameters, infrared LLLT proved to be a valuable alternative for BMS treatment, providing a significant and lasting reduction in symptoms.


Subject(s)
Burning Mouth Syndrome/radiotherapy , Low-Level Light Therapy , Adult , Aged , Female , Humans , Male , Middle Aged , Pain Measurement , Pilot Projects
11.
Photomed Laser Surg ; 22(6): 489-93, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15684748

ABSTRACT

OBJECTIVE: This in vitro study examines the efficacy of two different dental whitening agents, Opalescence Xtra and Opus White, by analyzing the change in color achieved by the treatment and the temperature increase induced in the pulpal chamber. BACKGROUND DATA: Bleaching techniques achieved significant advances with the use of coherent or incoherent radiation sources to activate the bleaching chemicals. METHODS: The bleaching agents, containing 35% of hydrogen peroxide, were stimulated with 0.9 W of xenon arc lamp and 0.9 W or 2 W of a 960-nm diode laser during 60 sec (0.9 W) and 30 sec (2 W) on 33 extracted human teeth. During irradiation, the temperature in the pulpal cavity was monitored. The color change was evaluated using the CIE L*a*b* color space measurement system. RESULTS: The treated groups showed an increase in color saturation (DeltaC*) of 3-32% and a change in whiteness (DeltaL*) of 0-8%. This study found that only some of the irradiated groups show statistically significant difference (p < 0.05) in the effectiveness of their treatment when compared to the control, whereas no significant statistical difference was obtained in between the irradiated groups. Temperature increase was 2-4 degrees C when using the xenon arc lamp, 2-8 degrees C and 4-12 degrees C when using the diode laser at 0.9 W and 2 W, respectively. CONCLUSIONS: The results of this study suggest that Opalescence Extra and Opus White are both effective to provide brighter teeth. However, according to the conditions used in this study, only the xenon arc lamp induced a safer temperature increase.


Subject(s)
Hot Temperature/therapeutic use , Laser Therapy , Peroxides/radiation effects , Polyvinyls/radiation effects , Tooth Bleaching , Urea/analogs & derivatives , Urea/radiation effects , Body Temperature , Color , Dental Pulp , Drug Combinations , Humans , Tooth Bleaching/methods , Xenon
SELECTION OF CITATIONS
SEARCH DETAIL
...