Epidemiology of bovine brucellosis by a combination of rose bengal test and indirect ELISA in the five districts of Uganda.

A serological survey on bovine brucellosis was carried out 3 times between 2007 and 2009 in 3 districts (Kiboga, Mpigi and Kiruhura) in western Uganda and 2 (Kumi and Mbale) in the east employing the rose bengal test (RBT) for infected-herd screening and an indirect ELISA (iELISA) for testing the serostatus of individual animals. The animal prevalence was significantly higher in the 3 districts of the west (mean 21.5% in 2009) compared with the 2 districts (mean 3.4% in 2008) in the east (P<0.0001), though a significant difference was not observed between Kumi and Mpigi in 2008. In the west, it was the lowest in Mpigi, but a significant increase was observed between 2008 (5.3%) and 2009 (30.0%), as in Kiruhura, in which the prevalence increased from 8.1% in 2007 to 16.8% in 2009. A similar trend was also observed in Kumi, namely, the seropositivity significantly increased from 2.3% in 2007 to 6.2% in 2008 and became remarkably higher than in Mbale (0.64%). As a result, the farm prevalence was also higher in the west, especially in Kiboga in 2007 (77.8%) and 2008 (65.6%), and Mpigi in 2009 (70.8%). The linear predictor of the fitted generalized linear model proved that the logit of RBT positivity increased linearly over the increase in percent positivity values. This study demonstrated an example of an unaided self-help survey as one of the control measures in Uganda.

Effects of season and agro-ecological zone on the microbial quality of raw milk along the various levels of the value chain in Uganda.

Dairy production in Uganda is pasture-based and traditional Ankole cattle make up 80% of the cattle herd, reared in both pastoral and agro-pastoral ecological zones. Regardless of the zone, milk quality is lowest in production basin during the dry season when ambient temperatures are highest and water is scarce. Poor hygiene and quality management contributed to the deterioration of raw milk quality during its storage and delivery to the final consumer, and concealed the seasonal effect when milk reached urban consumption areas. Poor milk quality is a challenge for the Ugandan Dairy Development Authorities who wish to make the milk value chain safe. This study provides baseline information for the implementation of an HACCP-based system to ensure the hygienic quality of milk from the farm to the market place.