Halo-perfluoroalkoxylation of gem-difluoroalkenes with short-lived alkali metal perfluoroalkoxides in triglyme.

Alkali metal alkoxides play a pivotal role in nucleophilic alkoxylation reactions, offering pathways for the synthesis of ethers, including the increasingly sought-after trifluoromethyl ethers. However, the synthesis of long-chain perfluoroalkyl ethers remains a substantial challenge in this field. Through the innovative use of triglyme to encapsulate potassium ions, we enhanced the stability of short-lived, longer-chain perfluoroalkoxy anions, thereby facilitating efficient nucleophilic perfluoroalkoxylation reactions. This method provides a new precedent for the halo-perfluoroalkoxylation of gem-difluoroalkenes and offers a versatile tool for the design of perfluoroalkyl ethers, including those containing complex moieties of heterocycles and drug molecules. We also demonstrated the utility of the resulting halo-perfluoroalkoxyl adducts through various chemical transformations to valuable diverse perfluoroalkyl ethers.

Effects of repetitive insertion/removal cycles and simulated occlusal loads on retention of denture retainers.

The purpose of this study was to investigate the effects of repetitive insertion/removal cycle tests on denture retainers with simulated occlusal loads on the retentive force and deformation of clasp. Abutment teeth in the form of mandibular secondary premolars and clasp in the form of Akers clasps were prepared. The retentive force of the clasp on the abutment teeth were evaluated before and after undergoing repetitive insertion/removal cycle tests with or without cyclic loading. Changes in the clasp shape were monitored using a 3D scanner and scanning electron microscope. The initial retentive force was approximately 10 N and this value later decreased due to deformation of the clasp tips. In contrast to the non-load group, the load group exhibited a reduction in retentive force during earlier stages. Therefore, cyclic loading was related to a decrease in retentive forces, specifically in the early stages of repetitive insertion/removal cycles.

Influence of reinforcement bar on accuracy of removable partial denture framework fabricated by casting with a 3D-printed pattern and selective laser sintering.

PURPOSE: The purpose of this study was to investigate the accuracy of removable partial denture frameworks fabricated by 3D-printed pattern casting (AM-Cast) and selective laser sintering (SLS) under different co nditions with a reinforcement bar. METHODS: A partially edentulous model was scanned with a dental laboratory scanner, and CAD software was used to design the framework. Reinforcement bars (n=0-2) were set on the lingual side of the framework. 3D scanning of the fabricated frameworks by AM-Cast and SLS was performed, and the obtained data were overlapped with the design data. The differences in shape among setting conditions of the bar were statistically compared using the Bonferroni method after the Kruskal-Wallis test. RESULTS: The ranges in differences of the AM-Cast-0, AM-Cast-1, and AM-Cast-2 were -0.167 to 0.128 mm, -0.101 to 0.105 mm, and -0.185 to 0.015 mm, respectively. The ranges of SLS-0, SLS-1, and SLS-2 were -0.166 to 0.035 mm, -0.182 to 0.049 mm, and -0.138 to 0.038 mm, respectively. Large discrepancies were observed at the joining area of the lingual bar on the right side of the AM-Cast. A significant difference was found between the AM-Cast-0 and AM-Cast-1, and between the AM-Cast-0 and AM-Cast-2. CONCLUSIONS: The setting conditions of the reinforcement bar affected the accuracy of the lingual bar in the AM-Cast; however, no effect was observed on the displacement of the central area of the lingual bar in SLS. Setting a single reinforcing bar on the retentive latticework contributed to improving the accuracy of the lingual bar in the AM-Cast but not the displacement of the central area of the lingual bar in SLS.

Accuracy of removable partial denture framework fabricated by casting with a 3D printed pattern and selective laser sintering.

PURPOSE: The present study aimed to compare the accuracy of removable partial denture (RPD) frameworks fabricated by 3D-printed pattern casting and those fabricated by selective laser sintering (SLS). METHODS: A partially edentulous mandibular model was used for the simulation model. Scanning of the model was performed using a dental scanner. The framework was designed by using CAD software. The 3D-printed resin pattern was formed using a 3D printer and casting was performed (AM-Cast framework), and a direct metal laser sintering machine was used for the framework of SLS (SLS framework). 3D scanning of fabricated two types of framework were performed, and these data were overlapped with design data. Fabrication accuracy was verified using the Mann-Whitney U test to compare the discrepancy between the AM-Cast and SLS frameworks. RESULTS: The range of differences for the AM-Cast and SLS framework were -0.185±0.138 to 0.352±0.143mm and -0.166±0.009 to 0.123±0.009mm, respectively. Statistically significant differences were observed at the rests, proximal plates, connectors, and clasp arms. Regarding the rests, both lateral and medial displacement in the two types framework was observed in relation to the design data. Large lateral discrepancies of the connectors were observed at the joining area on the tooth-supported side of the lingual bar for the AM-Cast framework. Localized discrepancies were observed at the center of the lingual bar for the SLS framework. CONCLUSION: The accuracies of RPD frameworks fabricated by AM-Cast and SLS differ depending on the specific structural component of the RPD.

Accuracy of Clasps Fabricated with Three Different CAD/CAM Technologies: Casting, Milling, and Selective Laser Sintering.

PURPOSE: To compare the fabrication accuracy of clasps made using different CAD/CAM technologies. MATERIALS AND METHODS: Five clasps were created using three CAD/CAM technologies: casting from a resin pattern using additive manufacturing; computer numerical controlled milling; and selective laser sintering (SLS). Differences between the scan data of the clasps and the CAD data were statistically analyzed. RESULTS: There were significant differences in all parts of the clasps. The difference values were small overall for the SLS clasp. CONCLUSION: This study demonstrated that SLS for clasp fabrication is outstanding in terms of fabrication accuracy and reproducibility.

Resistance of Fetal Guinea Pig Ventricular Myocardium to Hypoxia: Maintained Intracellular ATP Prevents the Opening of ATP-Sensitive Potassium Channels.

The presence and function of the ATP-sensitive potassium channel current (IKATP) were examined in the guinea pig myocardium to clarify the mechanisms for the resistance of the fetal myocardium to hypoxia. Experimental hypoxia markedly reduced the action potential duration and contractile force in isolated ventricular myocardium from the adult, but only moderately in those from the fetus. In isolated ventricular cardiomyocytes, the density of the IKATP activated by cromakalim, as well as their sensitivity to intracellular ATP concentration, were not different between the fetus and adult. The tissue ATP content was similar between the fetal and adult myocardium under normal condition, but the hypoxia-induced decrease was smaller in the fetus. Confocal microscopic analysis revealed that the mitochondria in the fetal cardiomyocyte is less in quantity than that in the adult and is more localized to the cell center. These results indicate that IKATP in the fetal guinea pig myocardium has a current density and ATP sensitivity similar to those of the adult, but is not activated under hypoxic conditions because the energy metabolism of the fetal myocardium is less dependent on oxidative phosphorylation.

Discovery of novel 5,5-diarylpentadienamides as orally available transient receptor potential vanilloid 1 (TRPV1) antagonists.

We have developed a novel and potent chemical series of 5,5-diphenylpentadienamides for targeting TRPV1 in vitro and in vivo. In this investigation, we examined a variety of replacements for the 5-position of dienamides with the goal of addressing issues related to pharmacokinetics. Our data suggest that substitution with alkoxy groups on the phenyl ring at the 5-position increases their ability to penetrate the blood-brain barrier. This investigation culminated in the discovery of compound (R)-36b, which showed a good pharmacokinetic profile. In vivo, compound (R)-36b was found to be effective at reversing mechanical allodynia in rats in a dose-dependent manner, and it reversed thermal hyperalgesia in a model of neuropathic pain induced by sciatic nerve injury.

H2receptor-mediated positive inotropic effect of histamine in neonatal guinea-pig left atria.

The receptor type mediating the positive inotropic effect of histamine was examined in left atria from neonatal guinea pigs. The positive inotropic effect of histamine, as well as its action potential prolonging effect, was antagonized by ranitidine, but not by chlorpheniramine or thiperamide. The positive inotropic effect was enhanced by isobutylmethylxantine. Receptor binding studies revealed the presence of both H1 and H2 receptor types. These results suggest that the positive inotropic effect of histamine in the neonatal guinea-pig atrium is mediated by H2 receptors.