Subject(s)
AIDS-Related Opportunistic Infections/diagnosis , Aspergillosis/diagnosis , Aspergillus fumigatus/isolation & purification , Deafness/microbiology , Facial Paralysis/microbiology , Temporal Bone/microbiology , AIDS-Related Opportunistic Infections/epidemiology , AIDS-Related Opportunistic Infections/therapy , Aspergillosis/epidemiology , Aspergillosis/therapy , Humans , Male , Middle AgedABSTRACT
A 25-year retrospective study of 63 pediatric surgical cases of benign parotid disease was done. Inflammatory disorders accounted for 34 of the cases. The remaining 29 non-inflammatory conditions included vasoformative, solid, and cystic lesions and were nearly always asymptomatic.
Subject(s)
Parotid Diseases , Abscess/diagnosis , Abscess/therapy , Adolescent , Child , Child, Preschool , Cysts/diagnosis , Cysts/surgery , Female , Hemangioma/diagnosis , Hemangioma/pathology , Hemangioma/therapy , Humans , Infant , Lymphangioma/diagnosis , Lymphangioma/pathology , Lymphangioma/therapy , Male , Medical History Taking , Palpation , Parotid Diseases/classification , Parotid Diseases/diagnosis , Parotid Diseases/pathology , Parotid Diseases/surgery , Parotid Neoplasms/diagnosis , Parotid Neoplasms/pathology , Parotid Neoplasms/surgery , Parotitis/diagnosis , Parotitis/pathology , Parotitis/surgery , Physical ExaminationABSTRACT
The synthesis of indole-3-acetic acid (IAA) in the enzyme extracts of Nicotiana glauca, Nicotiana langsdorffii, their F1 hybrid, their amphidiploid hybrid, and the nontumorous mutant of the hybrid was investigated. Tryptamine, a possible precursor of IAA biosynthesis in Nicotiana tabacum, was not found in the callus tissue of N. glauca, N. langsdorffii, and their F1 hybrid.In petiole slices, the synthesis of IAA progressively increased during 5 hours of incubation in [(14)C]tryptophan. The rate of synthesis was about equal in the hybrid and N. langsdorffii but lower in N. glauca on either a cell or fresh weight basis. It was also found that tryptophan was about 25 times more efficient than tryptamine in promoting synthesis of IAA in petiole slices.It was found that indoleacetaldehyde oxidase, indoleacetaldehyde reductase, and tryptophan aminotransferase activities were present in all of the species examined; however, tryptophan decarboxylase activity was not found. The tryptophan aminotransferase activity in N. glauca, N. langsdorffii, and the nontumorous mutant required alpha-ketoglutaric acid and pyridoxal 5-phosphate whereas the addition of pyridoxal 5-phosphate seemed not to increase the enzyme activity in tumor plants.The tryptophan aminotransferase in the amphidiploid hybrid was partially purified by acetone precipitation. The enzyme activity had a temperature optimum at 49 C and a pH optimum at 8.9. It is suggested that there is an indolepyruvic acid pathway in the synthesis of IAA in the Nicotiana species examined.
