ABSTRACT
A strain of Japanese encephalitis (JE) virus has been isolated from a pool of female mosquitoes of C. tritaeniorhynchus, using C. bitaeniorhynchus cell line. This is the first report of JE virus isolation from mosquitoes in Gorakhpur district of Uttar Pradesh, north India.
Subject(s)
Culicidae/virology , Encephalitis Virus, Japanese/isolation & purification , Animals , Female , India , Mice , Virus Cultivation/methodsABSTRACT
A survey of ixodid ticks was carried on in 1977 to determine the crimean haemorrhagic fever (CHF) virus activity in Jammu & Kashmir state, India. In all, 412 immatures and 3258 adults belonging to 7 genera and 16 species of ticks were collected as ectoparasites of small and large mammals. These included one species each of the genera Boophilus, Dermacentor and Nosomma, 2 species of Rhipicephalus, 3 species of Ixodes and 4 species each of Haemaphysalis and Hyalomma. Haem. bispinosa was the predominant species followed by B. microplus, Haem. montgomeryi, R. haemaphysaloides and Hyal. m. isaaci. The four Hyalomma species viz., Hyal. a. anatolicum (incriminated as the vector of CHF virus), Hyal. detritum, Hyal. dromedarii and Hyal. m. isaaci were found parasitizing goat, sheep, camel and cattle and were collected in the districts of Jammu, Rajouri, Poonch and Udhampur. Four species viz., Haem. intermedia, Hyal. detritum, Hyal. dromedarii and Hyal. m. isaaci are new records for the state. A total of 138 pools comprising eight species under six genera of ticks were processed for isolating the virus. CHF virus was not isolated. The role of ixodid ticks, particularly those of the genus Hyalomma in the dissemination of CHF virus is highlighted.
Subject(s)
Bunyaviridae/isolation & purification , Hemorrhagic Fever Virus, Crimean-Congo/isolation & purification , Mammals/parasitology , Tick Infestations/veterinary , Ticks/microbiology , Animals , Female , India , Larva/microbiology , Male , Nymph/microbiology , Tick Infestations/parasitologyABSTRACT
Tubers of three potato cultivars were stored at room temperature (20-39 degrees C, 30-75% RH), under an insulated storage run on passive evaporative cooling (16-30 degrees C, 70-90% RH) and a refrigerated storage (2-4 degrees C, 90-95% RH) for 14 weeks and studied periodically for storage losses, reducing sugar content and dry matter percent of tubers, in order to explore the possibility of storing potatoes for processing at higher temperatures. Physiological losses in tubers remained less than 10% until 12 weeks of storage under evaporatively cooled storage. Reducing sugar contents increased by only 52.4-242.1% in tubers stored in evaporatively cooled storage as compared to 90.5-484.2% increase in tubers stored in refrigerated storage until 14 weeks. Potatoes stored in evaporatively cooled store were more suitable for processing into chips and french fries due to lower physiological losses and lower reducing sugar content of tubers.