ABSTRACT
A simple method for preparing human and animal fetal pancreatic cell cultures has been developed. It is based on enzymatic treatment of the fetal pancreatic tissue with collalitine in combination with microdissection. As a result of subsequent cultivation there form floating cytotypic and organotypic cultures consisting mainly of B cells in different phases of the secretory cycle. The floating cultures prepared by the above-described method produce insulin and can be successfully used in experimental and clinical transplantology.
Subject(s)
Culture Techniques/methods , Pancreas/embryology , Humans , Pancreas/cytologySubject(s)
Diabetes Mellitus/surgery , Islets of Langerhans Transplantation , Abdominal Muscles/surgery , Adolescent , Adult , Blood Glucose/analysis , Cells, Cultured , Diabetes Mellitus/blood , Evaluation Studies as Topic , Female , Humans , Insulin/administration & dosage , Male , Middle Aged , Spleen/surgery , Time FactorsSubject(s)
Arm/physiology , Physical Exertion , Posture , Ventricular Function , Adult , Blood Pressure , Humans , Male , Oxygen/bloodABSTRACT
Before and after a 5 day head-down tilt of 4.5 degrees blood samples were withdrawn from different areas of the cardiovascular system of healthy male volunteers. Blood was sampled by means of selective catheterization. Parameters of acid-base equilibrium and activities of aminotransferases and alkaline phosphatase were measured. After immobilization the most significant changes were observed in the blood outflowing from the brain in which significant metabolic acidosis and increased activity of aspartate aminotransferase occurred. A slight increase in the activity of aspartate aminotransferase was also observed in the blood outflowing from the liver and kidneys. It is concluded that brain is the organ which is subjected to short-term simulated weightlessness in the highest degree.
