ABSTRACT
Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C). Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature. Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature. The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature. EAI and WAC of FG samples reduced significantly with rise in extraction temperature. Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
Subject(s)
Flax/chemistry , Plant Gums/chemistry , Seeds/chemistry , Temperature , Chemical Phenomena , Emulsions/chemistry , Food Handling/methods , Microscopy, Electron, Scanning , Monosaccharides/analysis , Protein Denaturation , Water/chemistryABSTRACT
Flaxseed protein isolate (FPI) was extracted from flaxseeds, and its amino acid composition and functional properties (solubility, thermal stability, emulsifying properties and electrostatic charge density, water holding and fat absorption capacities) were determined. The highest purity of FPI (90.6%) was achieved by extraction at 60°C. FPI had a low lysine to arginine ratio of 0.25, which is desired in heart-healthy foods and infant formulas. The denaturation temperature of FPI was 105°C. FPI had the highest emulsion activity index (375.51 m(2)/g), highest emulsion stability index (179.5 h) and zeta potential (-67.4 mV) when compared to those of other commonly used proteins, such as sodium caseinate (SC), whey protein isolate (WPI), gelatin (Gel) and soy protein isolate (SPI). The average emulsion droplet size of emulsions stabilized by these proteins was in the order SCSubject(s)
Amino Acids/analysis
, Flax/chemistry
, Plant Proteins/isolation & purification
, Seeds/chemistry
, Caseins/chemistry
, Emulsions
, Plant Proteins/chemistry
, Protein Stability
, Solubility
, Soybean Proteins/chemistry
, Static Electricity
, Surface Properties
, Temperature
, Water/chemistry
, Whey Proteins/chemistry
