ABSTRACT
In this study 252 poultry samples comprised of poultry meat (n = 228) and poultry eggs (n = 24) were screened for the isolation of Escherichia coli (E. coli). A total of 62 E. coli isolates were recovered from poultry meat. The E. coli isolates belonged to different serogroups based on O serotyping of the isolates viz O29 (10.8%), O8 (7.7%), O40 (6.15%), O2 (4.61%), O60 (3.08%), O106 (3.08%), 42 (1.54%), O 87 (1.54%), and 01 serotypes of O1, O7, O30, O45, O59, O66, O105, O1116, O136, O141, O147, O148, O166, and O172. Sixteen (24.62%) of the isolates were UT (untypable) and 6 (9.23 %) were rough types. Molecular characterisation of the isolates was performed, targeting stx1 and stx2 virulence gene fragment. Out of 62 E. coli isolates, 10 (16.12%) were carrying virulence gene stx2, whereas none of the isolate was carrying stx1 gene. The E. coli isolates showed wide variation in resistance pattern against the antimicrobial agents that we used (9-90%). Among E. coli isolates, maximum resistance was observed against cefuroxime (89.1%) and penicillin (89.4%), followed by ampicillin (80.43%), vancomycin (74.1%), co-trimoxazole (73.1%), cephalothin (60.8%), ceftriaxone (28.2%), tetracycline (17.4%), gentamicin (13%), amikacin (13.04%), ofloxacin (13%), and ciprofloxacin (6.5%). A high degree of susceptibility was observed against amikacin (84.7%) and ciprofloxacin (76%) followed by gentamicin (71.73) and ofloxacin (60.86%). High multiple antibiotic resistances were observed and a total of 34 resistance patterns were identified.
Subject(s)
Anti-Bacterial Agents/pharmacology , Eggs/microbiology , Escherichia coli/classification , Escherichia coli/drug effects , Food Microbiology , Meat/microbiology , Poultry/microbiology , Animals , Drug Resistance, Bacterial/genetics , Escherichia coli/genetics , Escherichia coli/isolation & purification , Genes, Bacterial/physiology , India , Microbial Sensitivity TestsSubject(s)
Anti-Bacterial Agents/pharmacology , DNA Gyrase/genetics , DNA Topoisomerases/genetics , Drug Resistance, Bacterial , Fluoroquinolones/pharmacology , Salmonella Infections, Animal/microbiology , Salmonella typhimurium/drug effects , Salmonella typhimurium/enzymology , Animals , Buffaloes , DNA Gyrase/metabolism , Feces/microbiology , India , Meat/microbiology , Mutation , Poultry , Salmonella typhimurium/genetics , Salmonella typhimurium/isolation & purificationABSTRACT
AIM: This study was conducted to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic resistance pattern. MATERIALS AND METHODS: A total of 136 samples (skin and nasal swab) from cattle and buffalo were collected. MRSA was identified by conventional bacterial culture techniques which were further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction (PCR). The isolates were further analyzed for the presence of mec A gene by PCR. The antimicrobial susceptibility profiling was performed by disc diffusion method. RESULTS: The prevalence of MRSA in the current study was 28.57% and 34.28% in cattle nasal and skin swab, respectively, with an overall prevalence of 31.43% MRSA among cattle. Buffalo nasal and skin sample showed MRSA prevalence of 54.55% and 39.4%, respectively, with 46.9% overall prevalence. PCR could detect mec A gene in 36.4% and 58% MRSA isolates from cattle and buffalo, respectively. Antimicrobial susceptibility test found MRSA resistant to penicillin and oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%). 100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin. Three (16.7%) MRSA isolates from buffalo were found resistant to vancomycin. CONCLUSION: Cattle and buffalo were identified as a potential carrier of MRSA in Bihar (India). The isolation of vancomycin-resistant S. aureus (VRSA) in the current study indicates the emergence of VRSA in animal population which may be transmitted to the human beings working in close contact to the animals.
ABSTRACT
Brucella abortus, a gram negative, facultative intracellular pathogen causes brucellosis in many animal species and humans. Although live, attenuated vaccines are available against this infection, they suffer from certain limitations. Therefore, the development of an effective subunit vaccine against brucellosis is an area of intense research. The outer membrane proteins (OMPs) of Brucella species have been extensively studied for its immunogenicity and protective ability. We have investigated the potential of CpG ODN to enhance the immunogenicity and protective efficacy of recombinant 28 kDa outer membrane protein (rOMP28) of Brucella melitensis. The study demonstrated vigorous immunoglobulin G (IgG) response of OMP28. The administration of rOMP28 with CpG caused increased cell mediated immune response in terms of induced IgG2a, T-cell proliferation and up-regulation of type I cytokine expression. In contrast, the free antigen suppressed the interferon gamma (type I cytokine) production on in-vitro stimulation of spleenocytes. The result indicates the role of OMP28 in the down regulation of IFN-gamma production. Moreover, the B. abortus S-19 vaccinated mice showed highest production of IL-4 and IFN-gamma. The protective ability of the antigen was evaluated by systemic bacterial clearance after challenging the mouse with B. abortus 544 pathogen. The level of protection was significant in rOMP28+CpG treated mice but was lower than the required level. The results of the present study indicate that rOMP28 could be an immunogen capable of inducing both humoral and cellular immune response. The humoral response was biased towards Th1 type when it was co-administered with CpG.
