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1.
Am J Physiol ; 269(5 Pt 1): C1105-11, 1995 Nov.
Article in English | MEDLINE | ID: mdl-7491897

ABSTRACT

Changes in a membrane sterol exchange of sickle red blood cells (SS RBC) induced by deoxygenation were studied using the fluorescent cholesterol analogue dehydroergosterol (DHE). DHE uptake by SS RBC membrane was measured by the incubation of SS RBC with small unilamellar vesicles (SUV) containing DHE. Deoxygenation of SS RBC, but not normal RBC, increased the rate of DHE uptake. DHE membrane content after 5 h of incubation with SUV in the cell-to-SUV ratio of 1:1 (mol lipid) was 16.25 +/- 0.94 and 12.22 +/- 0.85% of total sterol for deoxygenated and oxygenated cells, respectively. Membrane spicules isolated from these deoxygenated SS RBC had three-fold higher DHE content, suggesting that the increased sterol exchange was localized to spicules. When isolated spicules were incubated with DHE-SUV directly, 91 +/- 3% of membrane sterol was rapidly exchanged, in contrast to intact RBC, in which a maximum of 33% of sterol could be exchanged. The results suggest that spicule formation in SS RBC alters membrane cholesterol structure, such that a domain of cholesterol that is normally nonexchangeable becomes readily exchangeable with exogenous sterol.


Subject(s)
Anemia, Sickle Cell/blood , Cholesterol/blood , Erythrocyte Membrane/metabolism , Oxygen/metabolism , Chromatography, High Pressure Liquid , Ergosterol/analogs & derivatives , Ergosterol/metabolism , Fluorescence , Humans , Membrane Lipids/metabolism , Sterols/metabolism
2.
Mol Membr Biol ; 12(1): 113-9, 1995.
Article in English | MEDLINE | ID: mdl-7767369

ABSTRACT

Membrane cholesterol is distributed asymmetrically both within the cell or within cellular membranes. Elaboration of intracellular cholesterol trafficking, targeting and intramembrane distribution has been spurred by both molecular and structural approaches. The expression of recombinant sterol carrier proteins in L-cell fibroblasts has been especially useful in demonstrating for the first time that such proteins actually elicit intracellular and intraplasma membrane redistribution of sterol. Additional advances in the use of native fluorescent sterols allowed resolution of transbilayer and lateral cholesterol domains in plasma membranes from cultured fibroblasts, brain synaptosomes and erythrocytes. In all three cell surface membranes, cholesterol is enriched in the inner, cytofacial leaflet. Up to three different cholesterol domains have been identified in the lateral plane of the plasma membrane: a fast exchanging domain comprising less than 10% of cholesterol, a slowly exchanging domain comprising about 30% of cholesterol, and a very slowly or non-exchangeable sterol domain comprising 50-60% of plasma membrane cholesterol. Factors modulating plasma membrane cholesterol domains include polyunsaturated fatty acids, expression of intracellular sterol carrier proteins, drugs such as ethanol, and several membrane pathologies (systemic lupus erythematosus, sickle cell anaemia and aging). Disturbances in plasma membrane cholesterol domains alter transbilayer fluidity gradients in plasma membranes. Such changes are associated with decreased Ca(2+)-ATPase and Na+, K(+)-ATPase activity. Thus, the size, dynamics and distribution of cholesterol domains within membranes not only regulate cholesterol efflux/influx but also modulate plasma membrane protein functions and receptor-effector coupled systems.


Subject(s)
Cell Membrane/chemistry , Cholesterol/metabolism , Adult , Anemia, Sickle Cell/blood , Anemia, Sickle Cell/metabolism , Anemia, Sickle Cell/pathology , Cell Membrane/metabolism , Cholesterol/analysis , Ergosterol/analogs & derivatives , Ergosterol/blood , Erythrocyte Aging , Erythrocytes/chemistry , Erythrocytes/metabolism , Erythrocytes/ultrastructure , Fluorescence Polarization , Half-Life , Humans , Lipid Bilayers/chemistry , Male
3.
Biochemistry ; 33(24): 7682-90, 1994 Jun 21.
Article in English | MEDLINE | ID: mdl-8011635

ABSTRACT

Sterols are not randomly distributed in membranes but appear to be localized in multiple kinetic domains. Factors that regulate these sterol domains are not well-understood. A recently developed fluorescence polarization assay that measures molecular sterol transfer [Butko, P., Hapala, I., Nemecz, G., of Schroeder, F. (1992) J. Biochem. Biophys. Methods 24, 15-37] was used to examine the mechanism whereby anionic phospholipids and liver sterol carrier protein-2 (SCP2) enhance sterol transfer. Two exchangeable and one very slowly or nonexchangeable sterol domain were resolved in phosphatidylcholine (POPC)/sterol small unilamellar vesicles (SUV). Inclusion of 10 mol % anionic phospholipids enhanced sterol exchange primarily by redistribution of sterol domain sizes rather than by alteration of half-times of exchange. This effect was dependent primarily on the percent content rather than the net charge per anionic phospholipid. In contrast, SCP2 simultaneously altered both the distribution of sterol molecules between kinetic domains and the exchange half-times of exchangeable sterol domains. The effects of SCP2 were much more pronounced when 10% acidic phospholipid was incorporated in the SUV. Compared to spontaneous sterol exchange, in the presence of 1.5 microM SCP2, the rapidly exchanging pool was increased by 36 to 330%, depending on the SUV phospholipid composition. Concomitantly, exchange half-times for rapidly and slowly exchangeable sterol were reduced by 60 to 98% for 1t1/2 and 14 to 85% for 2t1/2, respectively. The stimulatory effect of SCP2 was saturable and dependent both on protein concentration and on content of acidic phospholipids in membranes.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Carrier Proteins/pharmacology , Cholesterol/metabolism , Liposomes/metabolism , Membrane Lipids/metabolism , Neoplasm Proteins , Plant Proteins , Anions , Carrier Proteins/administration & dosage , Dose-Response Relationship, Drug , Drug Synergism , Electrochemistry , Fatty Acid-Binding Proteins , Fluorescence Polarization , Hydrogen-Ion Concentration , Kinetics , Liposomes/chemistry , Membrane Lipids/analysis , Phosphatidylcholines/analysis , Phosphatidylserines/analysis , Phospholipids/analysis , Phospholipids/pharmacology , Potassium Chloride/pharmacology
4.
Biochemistry ; 33(10): 2880-90, 1994 Mar 15.
Article in English | MEDLINE | ID: mdl-8130201

ABSTRACT

Structural domains of cholesterol and their regulation in the erythrocyte membrane are poorly understood. Dehydroergosterol fluorescence polarization change was used to continuously monitor the kinetics of sterol exchange and sterol domain size in erythrocyte ghost membranes. Direct correlation between molecular sterol exchange and steady-state dehydroergosterol fluorescence polarization measurements was obtained without separation of donor and acceptor membranes. Three important observations were made. First, sterol exchange between small unilamellar vesicles (SUV) with the same cholesterol/phospholipid ratio as the erythrocyte membrane (1-palmitoyl-2-oleoylphosphatidylcholine/cholesterol = 1:1) was resolved into three kinetic cholesterol domains: 23 +/- 9% of total sterol was rapidly exchangeable, with t1/2 = 23 +/- 6 min; 59 +/- 9% of total sterol was slowly exchangeable, with t1/2 = 135 +/- 3 min; and 19 +/- 9% of total sterol was essentially nonexchangeable, with a t1/2 of days. Second, the substitution of erythrocyte ghosts for SUV as an acceptor significantly altered the kinetic parameters of sterol exchange from donor SUV, graphically showing that both the properties of the acceptor and spontaneous desorption of cholesterol from the donor SUV influenced spontaneous cholesterol transfer. Third, studies of exchange between erythrocyte ghosts revealed multiple kinetic pools of sterol differing from those in the SUV: 4 +/- 2% of total sterol was rapidly exchangeable, with t1/2 = 32 +/- 9 min; 29 +/- 3% of total sterol was very slowly exchangeable, with t1/2 = 23 +/- 7 h; and a surprisingly large 67 +/- 2% of total sterol was nonexchangeable, with a t1/2 of days.


Subject(s)
Cholesterol/blood , Erythrocyte Membrane/chemistry , Membrane Lipids/blood , Ergosterol/analogs & derivatives , Erythrocyte Membrane/metabolism , Erythrocyte Membrane/ultrastructure , Fluorescence Polarization/methods , Humans , Kinetics , Liposomes , Mathematics , Models, Biological , Phosphatidylcholines , Sterols/blood
5.
Biochim Biophys Acta ; 1189(1): 52-60, 1994 Jan 03.
Article in English | MEDLINE | ID: mdl-8305459

ABSTRACT

The factors which regulate intermembrane sterol domains and exchange in biomembranes are not well understood. A new fluorescent sterol exchange assay allowed correlation of changes in polarization to sterol transfer. Analysis of spontaneous sterol exchange between L-cell plasma membranes indicated two exchangeable and one very slowly or nonexchangeable sterol domain. The exchangeable domains exhibited half-times of 23 and 140 min with fractional contributions of 5 and 30%, respectively. Sterol carrier protein-2 (SCP-2) enhanced sterol exchange between L-cell plasma membranes and altered sterol domain size in a concentration dependent manner. Previous model membrane studies indicate that SCP-2 alters sterol domains and exchange through interaction with anionic phospholipids. In contrast to these observations, the ionic shielding agents KCl, low pH, or neomycin were either totally or partially ineffective inhibitors of SCP-2 action in L-cell plasma membrane exchanges. Thus the mechanism of SCP-2 in sterol transfer appears to be less charge dependent in L-cell plasma membranes than in model membranes. The cholesterol lowering drug probucol was also capable of altering the sterol exchange kinetics.


Subject(s)
Carrier Proteins/metabolism , Cell Membrane/metabolism , Plant Proteins , Sterols/metabolism , Amino Acid Sequence , Animals , Cell Membrane/drug effects , Fluorescence Polarization , Hydrogen-Ion Concentration , L Cells , Mice , Molecular Sequence Data , Neomycin/pharmacology , Peptides/pharmacology , Potassium Chloride/pharmacology , Probucol/pharmacology
6.
Biochemistry ; 31(46): 11589-94, 1992 Nov 24.
Article in English | MEDLINE | ID: mdl-1445894

ABSTRACT

We have examined the idea that membrane enzymes are regulated by the viscosity of surrounding lipids using data compiled from the literature for the effect of the change in membrane viscosity ([symbol: see text]) at the gel- to liquid-crystal-phase transition on the activities of several enzymes. The analysis was not extended explicitly to the problem of viscosity-dependent regulation of membrane enzymes in liquid-crystalline lipids because of the absence of exact data for values of [symbol: see text] in liquid-crystalline phases of variable composition. For most membrane enzymes studied, energies of activation are discontinuous, while kcat is continuous, at the main-phase transition. We consider that the energy of activation contains terms related to the height of the chemical barrier to reaction and terms due to the mechanical properties of the bilayer, such as the work of expansion during the catalytic cycle and the temperature dependence of [symbol: see text]. We find that the differences in energies of activation, above and below the break points in Arrhenius plots, are orders of magnitude larger than can be accounted for by the above mechanical factors. Thus, discontinuities in energies of activation at the phase transition appear to reflect changes in the chemical barrier to reaction, which is independent of [symbol: see text]. The theorectical analysis indicates too that values of [symbol: see text] for bilayers in the liquid-crystalline phase would have to be several orders of magnitude larger than those for gel phases in order to provide a basis for viscosity-dependent regulation of membrane enzymes in liquid-crystalline phases.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Glucuronosyltransferase/chemistry , Membrane Fluidity , Membrane Proteins/chemistry , Animals , Cell Membrane/enzymology , Glucuronosyltransferase/metabolism , Lipid Bilayers , Membrane Proteins/metabolism , Microsomes, Liver/enzymology , Rats , Solvents , Temperature , Viscosity
7.
Biochemistry ; 31(1): 162-8, 1992 Jan 14.
Article in English | MEDLINE | ID: mdl-1731869

ABSTRACT

The effects of high pressure on the kinetic properties of microsomal UDP-glucuronosyltransferase (assayed with 1-naphthol as aglycon) were studied in the range of 0.001-2.2 kbar to clarify further the basis for regulating this enzyme in untreated microsomes. Activity changed in a discontinuous manner as a function of pressure. Activation occurred at pressure as low as 0.1 kbar, reaching one of two maxima at 0.2 kbar. As pressure was increased above 0.2 kbar, activity decreased, reaching a minimum at about 1.4 kbar followed by a second activation. The pathway for activation at pressure greater than 1.4 kbar was complex. The immediate effect of 2.2 kbar was nearly complete inhibition of activity. The inhibited state relaxed, however, over about 10 min (at 10 degrees C), to a state that was activated as compared with enzyme at 0.001 kbar or enzyme at pressures between 1.4 and 2.2 kbar, which was the highest pressure we could test. Examination of the detailed kinetic properties of UDP-glucuronosyltransferase indicated that the effects of pressure were due to selective stabilization of unique functional states of the enzyme at 0.2 and 2.2 kbar. Activation at 0.2 kbar was reversible when pressure was released. This was true as well as for activation at pressure greater than 1.4 kbar, but after prolonged treatment at 2.2 kbar, UDP-glucuronosyltransferase became activated irreversibly on release of pressure. The process by which prolonged treatment at 2.2 kbar led to permanent activation of UDP-glucuronosyltransferase after release of pressure was not reflected, however, by time-dependent changes in the functional state of UDP-glucuronosyltransferase at this pressure.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Glucuronosyltransferase/metabolism , Microsomes, Liver/enzymology , Animals , Catalysis , Enzyme Activation , Enzyme Stability , Glucuronosyltransferase/chemistry , Kinetics , Male , Pressure , Rats , Structure-Activity Relationship
8.
Bratisl Lek Listy ; 92(6): 301-3, 1991 Jun.
Article in Slovak | MEDLINE | ID: mdl-1651800

ABSTRACT

Bilayer lipid membranes (BLM) were used to reconstitute the hormonal reception system for insulin and glucagon. The effect of the hormones on both BLM and BLM with incorporated rat liver plasma membrane fragments (BLM-PM) was studied by measuring the elastic parameter of membranes--the Young modulus of elasticity in the direction perpendicular to the membrane plane (E perpendicular)--using an electroconstriction method. The effect of insulin (in a concentration of 10(-10) mol.l-1) on BLM-PM resulted in a much greater (about threefold) decrease of E perpendicular) compared to that on BLM. The high content of cholesterol had a strong inhibitory effect on this interaction. The effect of the insulin antagonist glucagon on BLM-PM resulted in opposite changes of the relative value of E perpendicular compared to the effect of insulin. While the effect of insulin resulted in a relative decrease of E perpendicular by about 60%, glucagon in the same concentration induced a relative increase of E perpendicular by about 8%.


Subject(s)
Cell Membrane/physiology , Membranes, Artificial , Receptor, Insulin/metabolism , Receptors, Gastrointestinal Hormone/metabolism , Animals , Cell Membrane/metabolism , Elasticity , Glucagon/metabolism , Lipid Bilayers , Rats , Receptors, Glucagon
9.
Biochemistry ; 29(25): 5961-7, 1990 Jun 26.
Article in English | MEDLINE | ID: mdl-2116902

ABSTRACT

Treatment of microsomes at pressures as high as 2.25 kbar led to an apparent irreversible activation of UDP-glucuronylsyltransferase when pressure was released. The response of the enzyme to pressure, as reflected by activity measured after release of pressure, appeared to be discontinuous in that no activation was seen for any preparation at pressures less than 1.2 kbar. In addition, activation was temperature dependent. Maximum activation at 2.25 kbar occurred at about 12 degrees C; the extent of activation in 10 min was less for either higher or lower temperatures. Activation was also time dependent. Maximum activation at 2.25 kbar and 9 degrees C required 90 min of pressure treatment. Activation appeared to occur more slowly at lower pressure. Pressure-induced activation was associated with a loss of sensitivity of the enzyme to allosteric activation by UDP-N-Ac-Glc and a conversion of the kinetic pattern from non-Michaelis-Menten to Michaelis-Menten. Pressure did not activate enzyme that had previously been activated maximally by adding detergent to microsomes. Pressure also did not activate pure UDP-glucuronosyltransferase reconstituted into unilamellar vesicles of dioleoylphosphatidylcholine. Pressure treatment did not release UDP-glucuronosyltransferase from microsomes into water. Pressure had a continuous effect on the polarization and excimer/monomer formation of fluorescent probes incorporated into microsomes, and the properties returned essentially to their values at 1 atm when pressure was released. Measurements of activity at 2.2 kbar showed that pressure-induced activation of UDP-glucuronosyltransferase in microsomes occurred via two intermediates that were inactive and that the activated state of the enzyme was generated during/after release of pressure.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Glucuronosyltransferase/metabolism , Microsomes, Liver/enzymology , Animals , Chemical Phenomena , Chemistry, Physical , Enzyme Activation , Lipids , Pressure , Rats , Temperature , Viscosity
10.
Czech Med ; 12(2): 101-16, 1989.
Article in English | MEDLINE | ID: mdl-2504559

ABSTRACT

The bilayer lipid membrane (BLM) was used to reconstitute the hormonal reception system for insulin. The effect of insulin on both unmodified BLM and BLM modified by rat liver plasma membrane fragments was studied by measuring the viscoelastic parameters of membranes--the modulus of elasticity in the direction perpendicular to the membrane plane, E perpendicular to, and the coefficient of dynamic viscosity, eta. The effect of insulin (in concentration of 10(-10) mol la1) on modified membranes resulted in a much greater (about 30-40%) decrease of E perpendicular compared to that on unmodified BLM. Analysis of the developed model of the membrane showed that in the vicinity of insulin--in the case of the unmodified BLM--and in the vicinity of hormone receptors--in the case of modified BLM--there appeared extensive regions of a changed membrane structure which could cause cooperative changes in the studied viscoelastic parameters of BLM. These changes were considerably influenced by the initial value of the BLM modulus of elasticity, fragment concentration and by the content of membrane cholesterol, which has a strong inhibitory effect. The effect of the insulin antagonist glucagon on modified BLM resulted in opposite changes of the relative value of E perpendicular compared to the effect of insulin.


Subject(s)
Insulin/metabolism , Lipid Bilayers , Membranes, Artificial , Receptor, Insulin/metabolism , Animals , Cell Membrane/metabolism , Elasticity , Glucagon/pharmacology , In Vitro Techniques , Insulin/pharmacology , Liver/metabolism , Liver/ultrastructure , Membrane Potentials , Rats
12.
Gen Physiol Biophys ; 7(2): 191-203, 1988 Apr.
Article in English | MEDLINE | ID: mdl-3292348

ABSTRACT

Insulin interaction with BLM with incorporated fragments of rat liver plasma membranes, containing hormone receptors, was studied by determining Young modulus of elasticity of bilayer lipid membranes in direction perpendicular to the surface, E. The presence of membrane proteins in a concentration of 60 micrograms.ml-1 induced a significant decrease in parameter E (to approx. 50%) as compared with values obtained in non-modified membranes during insulin action (concentration interval 10(-11)-10(-9) mol.l-1). The extent of the effect was dependent on the initial phase state of the membrane, on cholesterol content in BLM as well as on membrane proteins concentration in lipid bilayer.


Subject(s)
Insulin/pharmacology , Lipid Bilayers/metabolism , Animals , Cell Membrane/metabolism , Elasticity , Insulin/pharmacokinetics , Liver/metabolism , Rats , Solvents , Viscosity
13.
Gen Physiol Biophys ; 6(2): 173-83, 1987 Apr.
Article in English | MEDLINE | ID: mdl-3308630

ABSTRACT

Changes in the Young elasticity modulus in perpendicular direction to the membrane surface E perpendicular, in the coefficient of dynamic viscosity eta, in the electric capacitance C, in the surface charge U1, in the conductivity g and in the coefficient of non-linearity beta of current-voltage characteristic caused by insulin were studied in bilayer lipid membranes (BLM) prepared from a mixture of egg lecithin and cholesterol (4:1, w/w) in n-heptane. Even relatively small concentrations of insulin in electrolyte (ci approximately 4.8 x 10(-11) mol/l) caused a diminution in parameters E perpendicular and eta. Negative surface charge emerged on the membrane due to the insulin absorption, and U1 gradually increased depending on the concentration of the hormone in the electrolyte. Addition of insulin was also followed by an increase in membrane conductivity and affected the value of the coefficient of non-linearity beta of current-voltage characteristic. The effect of insulin on the BLM structure was discussed on the basis of the results obtained.


Subject(s)
Insulin/pharmacology , Lipid Bilayers , Elasticity , Electric Conductivity , Membrane Potentials , Surface Properties , Viscosity
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