Subject(s)
Camurati-Engelmann Syndrome/complications , Papilledema/complications , Retinal Diseases/etiology , Retinal Ganglion Cells/pathology , Basement Membrane/pathology , Camurati-Engelmann Syndrome/diagnosis , Female , Fluorescein Angiography , Fourier Analysis , Humans , Intracranial Pressure , Magnetic Resonance Imaging , Nerve Fibers/pathology , Papilledema/diagnosis , Retinal Diseases/diagnosis , Tomography, Optical Coherence , Tomography, X-Ray Computed , Visual Acuity/physiology , Young AdultABSTRACT
PURPOSE: Retinoids are associated with cell proliferation and differentiation. The levels of retinoids (vitamin A and its derivatives) in human amniotic membrane (AM) were measured to investigate whether endogenous retinoids in AM might contribute to the process of wound healing of the keratoconjunctival epithelium in AM transplantation. SUBJECTS AND METHODS: Retinoids were extracted from AM obtained from three patients following cesarean section. Retinoids including retinol (ROL), retinylaldehyde (RAL), retinoic acid(RA), and retinyl palmitate(RP) were analyzed using a reverse-phase high performance liquid chromatograph equipped with a multi-wavelength detector or fluorescence detector. RESULTS: ROL was identified and detected in AM at a concentration of 9.42 2.75(mean +/- standard deviation) ng/g wet tissue. No other retinoids, were detected. CONCLUSION: Among the retinoids tested, only retinol was detected at low levels in human AM tissues. Based on these results, endogenous retinoids in AM probably have little effect on wound healing of the keratoconjunctival epithelium in AM transplantation.
