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1.
FEBS Lett ; 590(6): 808-18, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26921582

ABSTRACT

Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) plays pathogenic roles in EBV-related diseases. Thus, host cells employ several mechanisms to regulate LMP1 functions, and we previously reported possible regulation by signal transducing adaptor protein-2 as well as BS69. Here, we found that caspase-3 mainly degraded LMP1 proteins in HeLa cells, leading to decreased NF-κB and STAT3 activation. Caspase-3 cleaved the consensus DNTD sequences in the CTAR3 region of LMP1. Of importance, LMP1 expression strongly enhanced caspase-3 activity. Taken together, the reduction of LMP1 protein levels by caspases is likely to be a newly identified host defense against EBV infection.


Subject(s)
Caspase 3/metabolism , Viral Matrix Proteins/metabolism , Caspase 3/genetics , Caspase Inhibitors/pharmacology , Enzyme Activation , Gene Expression , Genes, Viral , HeLa Cells , Herpesvirus 4, Human/genetics , Herpesvirus 4, Human/pathogenicity , Herpesvirus 4, Human/physiology , Host-Pathogen Interactions , Humans , NF-kappa B/metabolism , Oligopeptides/pharmacology , Protein Structure, Tertiary , Proteolysis , RNA, Small Interfering/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , STAT3 Transcription Factor/metabolism , Viral Matrix Proteins/chemistry , Viral Matrix Proteins/genetics
2.
J Virol ; 87(18): 10334-47, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23864627

ABSTRACT

Epstein-Barr virus (EBV), a human gammaherpesvirus, establishes a lifelong latent infection in B lymphocytes and epithelial cells following primary infection. Several lines of evidence suggest that exosomes derived from EBV-infected cells are internalized and transfer viral factors, including EBV-encoded latent membrane protein and microRNAs, to the recipient cells. However, the detailed mechanism by which exosomes are internalized and their physiological impact on the recipient cells are still poorly understood. In this study, we visualized the internalization of fluorescently labeled exosomes derived from EBV-uninfected and EBV-infected B cells of type I and type III latency into EBV-negative epithelial cells. In this way, we demonstrated that exosomes derived from all three cell types were internalized into the target cells in a similar fashion. Internalization of exosomes was significantly suppressed by treatment with an inhibitor of dynamin and also by the knockdown of caveolin-1. Labeled exosomes were colocalized with caveolae and subsequently trafficked through endocytic pathways. Moreover, we observed that exosomes derived from type III latency cells upregulated proliferation and expression of intercellular adhesion molecule 1 (ICAM-1) in the recipient cells more significantly than did those derived from EBV-negative and type I latency cells. We also identified the EBV latent membrane protein 1 (LMP1) gene as responsible for induction of ICAM-1 expression. Taken together, our data indicate that exosomes released from EBV-infected B cells are internalized via caveola-dependent endocytosis, which, in turn, contributes to phenotypic changes in the recipient cells through transferring one or more viral factors.


Subject(s)
B-Lymphocytes/virology , Caveolae/metabolism , Endocytosis , Epithelial Cells/virology , Exosomes/metabolism , Herpesvirus 4, Human/growth & development , B-Lymphocytes/metabolism , Cell Line , Epithelial Cells/metabolism , Humans
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