Evaluation of cell death and proliferation in psoriatic epidermis.

BACKGROUND: Previous studies of psoriatic epidermis using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) method, a type of apoptotic detection method, showed that TUNEL-positive keratinocytes were abundantly distributed in all layers of the psoriatic epidermis, although psoriasis is a hyperproliferative disorder. OBJECTIVE: We sought to clarify the nature of cell kinetics in a psoriatic epidermis on the basis of differences in the reactivities in TUNEL and formamide-induced DNA denaturation assay combined with the detection of denatured DNA with a monoclonal antibody (MAb) against single-stranded DNA (formamide-MAb assay) between the normal and psoriatic epidermides. METHODS: The kinetics of keratinocytes was evaluated by the immunohistochemistry of Ki-67 for proliferation activity and by TUNEL, TUNEL combined with transmission electron microscopy (TUNEL/TEM), and formamide-MAb assay for apoptosis. RESULTS: The number of Ki-67-positive cells in the psoriatic epidermis was significantly higher than that in the normal epidermis. In the normal epidermis, both TUNEL and formamide-MAb assay showed a similar distribution pattern, that is, both TUNEL and formamide-MAb assay-positive keratinocytes were present only in the upper granular layer. In the psoriatic epidermis, most keratinocytes were negative for the formamide-MAb assay, while TUNEL-positive cells were abundantly distributed in all layers of the psoriatic epidermis. TUNEL/TEM method clearly demonstrated that many immunogold particles that stain the sites of 3'-OH DNA ends were evenly distributed on the euchromatin in psoriatic keratinocyte nuclei, in contrast to their presence on the peripheral condensed chromatin in normal keratinocyte nuclei. CONCLUSION: The increased TUNEL reactivity in psoriatic lesions is due to the increase in the number of DNA nicks resulting from active DNA replication but not due to DNA double-strand breaks produced during the apoptotic process, and the formamide-MAb assay is a reliable method for the detection of apoptosis, particularly in the epidermis.

The activation of caspase-3 and DNA fragmentation in B cells phagocytosed by macrophages.

Apoptotic signaling of mammalian cells involves two pathways: the death receptor and mitochondrial pathways. In this in vivo study, we investigated apoptotic signaling of B cells in mouse germinal centers (GCs) of gut-associated lymphoid tissues (GALTs) using transmission electron microscopy (TEM), terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL), immunofluorescence of members of caspase family and cFLIP(L), and caspase activity assay. It was very difficult to ultrastructurally differentiate B cells undergoing apoptosis from B cells differentiating into memory cells or plasma cells among B cells constituting GCs. Isolated B cells in GCs showed no active form of caspase-3 or TUNEL immunoreactivity, but expressed cFLIP(L). Contrary to isolated B cells, apoptotic B cells phagocytosed by macrophages exhibited immunoreactivity of the active form of caspase-3 and TUNEL, but lacked the cFLIP(L) expression. The caspase activity assay in GALTs clearly showed intense activity of caspase-3, caspase-9, and caspace-8 that was high in order. Therefore, the death receptor pathway accompanying the increased activity of caspase-3 and caspase-8 may be blocked by the expression of cFLIP(L) in B cells of GALTs. Moreover, both the activation of caspase-3 and DNA fragmentation first occur only when B cells are phagocytosed by macrophages.