1.
Toxicon
; 27(7): 747-55, 1989.
Article
in English
| MEDLINE
| ID: mdl-2781574
ABSTRACT
A metalloprotease from Bothrops jararaca venom (J protease) was purified by DEAE-Sephacel, CM-cellulose, Sephacryl S-200 and Sephadex G-75 chromatograph. The proteolytic activity was inactivated by EDTA, o-phenanthroline and DTNB. Phosphoramidon and cysteine protease inhibitors (leupeptin, E64 and its derivatives) were inactive on this enzyme. J protease was activated by calcium and the metal content analysis showed the presence of one mole each of tightly bond zinc and calcium per mole of this J protease. The amino acid composition, N-terminal amino acid sequence (29 residues) and the cleavage sites on the oxidized insulin B chain and angiotensin I were determined.
Subject(s)
Crotalid Venoms/analysis , Metalloendopeptidases/isolation & purification , Zinc/metabolism , Amino Acid Sequence , Animals , Chromatography, DEAE-Cellulose , Chromatography, Gel , Chromatography, Ion Exchange , Enzyme Activation , Metalloendopeptidases/analysis , Metalloendopeptidases/antagonists & inhibitors , Metals/analysis , Molecular Sequence Data
2.
Toxicon
; 2(7): p.747-55, 1989.
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: but-ib8865