ABSTRACT
Under conditions of COVID-19 pandemic, considerable amounts of SARS-CoV-2 contained in household, municipal, and medical wastewaters inevitably reach natural water bodies. Possible preservation of virus infectivity in liquid environment is of a paramount epidemiological importance. Experiments demonstrated that SARS-CoV-2 is resistant to multiple freezing/thawing cycles and retains its infectivity in tap and river water for up to 2 days at 20°C and 7 days at 4°C. In natural milk, its viability is preserved in a refrigerator for 6 days. The exposure of aquarium fish to the virus-containing water fails to cause any infection.
Subject(s)
COVID-19 , Animals , Humans , Pandemics , SARS-CoV-2 , Wastewater , WaterABSTRACT
Different methods for producing bulk quantities of concentrated and purified SARS-CoV-2 for the use as antigens and for the research into COVID-19 biology were tested.
Subject(s)
COVID-19 Testing/methods , COVID-19/diagnosis , SARS-CoV-2/immunology , Viral Load/immunology , Virion/immunology , Animals , Antibodies, Neutralizing/isolation & purification , Antibodies, Neutralizing/metabolism , Antibodies, Viral/isolation & purification , Antibodies, Viral/metabolism , COVID-19/virology , Cell Line , Chlorocebus aethiops , Convalescence , Epithelial Cells/virology , Humans , Immune Sera/chemistry , SARS-CoV-2/genetics , SARS-CoV-2/growth & development , Swine , Vero Cells , Viral Load/genetics , Viral Proteins/immunology , Viral Proteins/isolation & purification , Viral Proteins/metabolism , Virion/genetics , Virion/growth & development , Virus ReplicationABSTRACT
The preparation and study of the biological properties of the pVAKS-GPVM DNA immunogen containing a gene encoding Marburgvirus glycoprotein are described. The specificity of blood serum antibodies of guinea pigs immunized with DNA immunogen was analyzed by ELISA. Inactivated viral preparation, recombinant glycoprotein (GP) obtained in the prokaryotic system and virus-like particles based on the recombinant vesicular stomatitis virus exhibiting Marburgvirus GP were used as the antigens. The neutralizing activity of antibodies of immunized animals was tested in vitro using a pseudovirus system. It was demonstrated that the developed immunogen administered to guinea pigs induced the production of specific antibodies that neutralize virus-like particles and Marburgvirus in cultured Vero cells.
